A Systematic Proteome Study of Seed Storage Proteins from Two Soybean Genotypes

Abstract

Soybean seed is a good source of plant protein in human consumables such as baby formula and protein concentrate. The seeds contain an abundance of storage proteins, namely β-conglycin and glycinin that account for ~ 70-80% of the total seed protein content. Proteome profiling has been proved to be an efficient way that can help us to investigate the seed storage proteins. In the present study, the seeds were removed from the pods and the cotylendonary tissues were separated from the testa for proteome analysis in order to investigate the seed storage proteins. A systematic proteome profiling was conducted through one-dimensional gel electrophoresis followed by MALDI-TOF-TOF mass spectrometry in the seeds (cotyledonary tissue) of soybean genotypes. Two dimensional gels stained with CBB, a total of 10 proteins were identified and analyzed using MASCOT search engine according to the similarity of sequences with previously characterized proteins along with the UniProt database. A total of ten proteins such as glycinin Gy4 precursor, glycinin G3 precursor, glycinin G1 precursor, glycinin chain A2B1a precursor, glycinin chain A2B1a precursor were identified in our investigation. However, the glycinin subunit may be considered to play important roles in soybean breeding and biochemical characterization. In addition, the improved technique will be useful to dissect the genetic control of glycinin expression in soybean.

Soybean genetic resources contributing to sustainable protein production

“…Glycinin subunits are believed to have an important role in soybean breeding and the improvement of the biochemical properties of soybean proteins. Cho et al (2014) identified 10 glycinins from the cotyledon tissue of the soybean seed coat, which provides a theoretical basis for clarifying the genetic regulation of glycinin expression in seeds. Min et al (2015) found that the urea cycle might be involved in the accumulation of glycinin and β-conglycinin subunits (SSPs), thereby increasing the protein content of soybean seeds.…”

Unfolding the Dynamics of Mineral Nutrients and Major Storage Protein Fractions during Soybean Seed Development

“…Albumin and globulin proteins account for ca. 70% of the soybean seed’s proteome. , Although similar biosynthesis trends have been previously observed, , most of these studies have focused on assessing the seed’s total protein content or the storage proteins on mature seeds as a function of different agricultural traits. − , Therefore, a precise profile of storage proteins biosynthesis throughout the seed development was poorly explored. The results presented herein confirm that the storage protein accumulation escalates during the cell elongation phases, i.e., in the aftermath of the cease in cell division and differentiation taking place up to the R 5.5 developmental stages (Figure ).…”

“…3,4 Notably, ca. 70% of the soybean seeds' proteome regards storage proteins, 5,6 which serves as an N source during seed germination and early-seedling development and are stowed in the protein bodies, a specialized group of vesicles located at seed cotyledon's cells. 7,8 These proteins are mainly constituted of water-soluble albumins and salt-soluble globulin fractions.…”

“…20%, weight basis). , Notably, ca. 70% of the soybean seeds’ proteome regards storage proteins, , which serves as an N source during seed germination and early-seedling development and are stowed in the protein bodies, a specialized group of vesicles located at seed cotyledon’s cells. , …”

Analysis of Protein Function and Comparison of Protein Expression of Different Environment in Soybean using Proteomics Techniques

“…함량이 2차 관계에서 차이를 보이며, 배수를 위한 고랑의 공간에 의해서도 콩의 생육과 생산량에 차이가 있다 (Cho et al, 2006;Rahman et al, 2011). 논에서 재배된 콩의 경우, 밭에서 재배된 콩과 비교하여 백립중과 콩 종실의 크기가 유의하게 증가하였다는 보고도 있다 (Kim et al, 2004 (Cho et al, 2014 (Woo et al, 2003 (Taira et al, 1977;Kim et al, 2004;Cho et al, 2006;Rahman et al, 2011…”