2020
DOI: 10.1038/s41598-020-74201-x
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A systematic identification of anti-inflammatory active components derived from Mu Dan Pi and their applications in inflammatory bowel disease

Abstract: Mu Dan Pi (MDP), also known as Moutan Cortex Radicis, is a traditional Chinese medicine used to treat autoimmune diseases. However, the impact of MDP and its principal active compounds on inflammatory bowel disease (IBD) is uncertain. This study therefore systemically assessed the anti-inflammatory effects of MDP and its known active compounds in IBD. The anti-inflammatory activities of water extract and individual compounds were screened by NF-κB and interferon regulatory factor (IRF) reporter assays in THP-1… Show more

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Cited by 13 publications
(13 citation statements)
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“…Next, we screened MDP fractions using cell-based reporter assays to identify the most effective fractions contributing to the anti-inflammatory effects of MDP. The monocytic cell line THP-1, which stably expresses NF-κB reporter constructs, was used as the reporter cells as previously described ( Chen et al, 2020 ). NF-κB reporter activity, enabling verification of the inflammatory response to the TLR2 agonist, Pam3CSK4, was used to measure inhibitory activity of each MDP fraction ( Figure 2 ).…”
Section: Resultsmentioning
confidence: 99%
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“…Next, we screened MDP fractions using cell-based reporter assays to identify the most effective fractions contributing to the anti-inflammatory effects of MDP. The monocytic cell line THP-1, which stably expresses NF-κB reporter constructs, was used as the reporter cells as previously described ( Chen et al, 2020 ). NF-κB reporter activity, enabling verification of the inflammatory response to the TLR2 agonist, Pam3CSK4, was used to measure inhibitory activity of each MDP fraction ( Figure 2 ).…”
Section: Resultsmentioning
confidence: 99%
“…THP-1 cells and reporter cells were cultured according to a previously described protocol ( Chen et al, 2020 ). Bone marrow-derived macrophages (BMDMs) were isolated from mice and differentiated for 5–6 days, following standard procedures ( Hsu et al, 2015 ).…”
Section: Methodsmentioning
confidence: 99%
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