A systematic assessment of goblet cell sampling of the bulbar conjunctiva by impression cytology

Doughty, Michael J.

doi:10.1016/j.exer.2015.04.007

Cited by 16 publications

(14 citation statements)

References 36 publications

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“…Goblet cells were quantified per 100 epithelial cells, as goblet cell density may vary with the overall epithelial cell density. Based on reported data in the human medical literature, we considered > 5% of goblet cells a normal frequency and we arbitrarily set the other percentages as a slight and a moderate decrease of globlet cells (2–5% and < 2%, respectively) . Brush smear slides were analyzed at 600× magnification and for the criteria described above.…”

Section: Methodsmentioning

confidence: 99%

Cytology of the healthy canine and feline ocular surface: comparison between cytobrush and impression technique

Perazzi

Bonsembiante

Gelain

et al. 2017

Veterinary Clinical Pathol

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Section: Methodsmentioning

confidence: 99%

Cytology of the healthy canine and feline ocular surface: comparison between cytobrush and impression technique

Perazzi

Bonsembiante

Gelain

et al. 2017

Veterinary Clinical Pathol

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“…Future studies should compare our results with those from cats with various ocular surface diseases and should consider techniques such as conjunctival impression cytology that provide a less traumatic manner of assessing GCD. [24][25][26]…”

Section: (A) (B) (C) (E) (D)mentioning

confidence: 99%

Goblet cell density and distribution in cats with clinically and histologically normal conjunctiva

Sebbag

Reilly

Eid

et al. 2016

Veterinary Ophthalmology

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Objective To evaluate goblet cell density (GCD) and distribution in cats without clinical evidence of ocular surface disease and without histologic evidence of conjunctival disease. Animals studied Fourteen domestic-shorthair cats euthanized for reasons unrelated to this study. Procedures Before euthanasia, cats were verified using slit-lamp biomicroscopy and fluorescein staining to be free of eyelid or ocular surface abnormalities. Immediately after euthanasia, bilateral conjunctival specimens including third eyelid (TEL) were collected, routinely processed, stained with Periodic acid-Schiff and hematoxylin and eosin. Thirteen conjunctival regions were identified. For each region, GCD was expressed as percentage of goblet cells/200 basal epithelial cells. Results Mean GCD ranged widely by region: anterior face of the TEL = 48.8%, fornicial regions = 47.0%, palpebral regions = 38.5%, bulbar regions = 19.6%, and posterior face of the TEL = 12.6%. The anterior surface of the TEL had significantly higher GCD than did the bulbar and the palpebral regions but not the fornicial regions. Bulbar conjunctiva had significantly lower GCD than did all other conjunctival regions except the posterior surface of the TEL. No significant difference was noted between GCD of male versus female cats, dorsal versus ventral regions or lateral versus medial regions. Conclusions Although conjunctival GCD ranged widely by region, the anterior surface of the TEL appears to be an excellent location for assessing conjunctival goblet cells in cats since this area has high GCD and is more readily accessible than are the palpebral, fornicial, or bulbar conjunctiva.

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“…ICS collects the most superficial layers of the ocular surface due to the adhering property of the sampling device [4]. ICS is now widely used in human ophthalmology to examine the healthy ocular surface [5] and to assess conjunctival and corneal alterations in contact lens wearers [6][7][8] Examination of eyes with ocular surface neoplasia [9,10] and keratoconjunctivitis sicca [11,12] are other indications for ICS. A major advantage of ICS is the arrangement of harvested cells in their natural layout; thus enabling clinical pathologists to examine an exact copy of the area of interest.…”

Section: Introductionmentioning

confidence: 99%

Impression cytology as diagnostic tool in horses with and without ocular surface disease

Braus

Lehenauer

Tichy

et al. 2016

Equine Veterinary Journal

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Summary Background Cytology plays a major role in the diagnosis of ocular surface diseases. Objective To compare 2 cytological sampling methods for obtaining corneal and conjunctival cell samples regarding irritation for the patient, invasiveness, sample quality and diagnostic equivalence. Study design Observational prospective study. Methods In 5 healthy horses, conjunctival and corneal samples were taken bilaterally by impression cytology sampling (ICS) and cytobrush sampling (CBS). Irritation and invasiveness were assessed with an eye irritation and an epithelial damaging score system, respectively. Sample quality was evaluated via morphometric analysis and graded by a board certified clinical pathologist. For the assessment of diagnostic equivalence, 15 eyes of 14 client owned horses with ocular surface anomalies were sampled by ICS and CBS. The methods were compared regarding the types of inflammatory cells and/or infectious agents detected and if the correct diagnosis could be achieved. Histopathology served as gold standard when available. Results ICS was significantly less invasive and less irritating for the horses. Both methods retrieved cells of overall high quality; the cell quantity was significantly higher in IC samples. ICS preserved the natural cellular layout. There was a fair, but no statistically significant agreement between the diagnostic outcomes between sampling methods although CBS resulted in a slightly greater variability of inflammatory cell types compared to ICS. Main limitations None. Conclusions Because of its low irritability and invasiveness ICS can be recommended for cell sampling in fragile corneas and for experimental studies. ICS is especially useful in cases where preservation of cellular layout is advantageous. CBS samples are easier to obtain because of the small equine palpebral fissure. Additionally, the identification of inflammatory cells within conjunctival cell samples is easier. Overall, CBS is still an appropriate method in clinical settings.

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A systematic assessment of goblet cell sampling of the bulbar conjunctiva by impression cytology

Cited by 16 publications

References 36 publications

Cytology of the healthy canine and feline ocular surface: comparison between cytobrush and impression technique

Cytology of the healthy canine and feline ocular surface: comparison between cytobrush and impression technique

Goblet cell density and distribution in cats with clinically and histologically normal conjunctiva

Impression cytology as diagnostic tool in horses with and without ocular surface disease

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