A systematic approach to the accurate quantification of selenium in serum selenoalbumin by HPLC–ICP-MS

Jitaru, Petru; Goenaga‐Infante, Heidi; Vaslin-Reimann, Sophie; Fisicaro, Paola

doi:10.1016/j.aca.2009.10.037

Cited by 73 publications

(41 citation statements)

References 25 publications

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“…34 The concentration in terms of selenium is 12.35 ± 0.92 ng g −1 when it is converted from SeMet to selenium. Other reports on SeAlb in blood serum 20,21,27 vary from 13 ng g −1 to 19 ng g −1 depending on samples and our result lies in the range studied by others.…”

supporting

confidence: 81%

“…Still, it should be pointed out that GPx might contain free SeMet and it should be proved that there is no free SeMet in the serum sample or free SeMet should be removed for the precise measurement of GPx. A previous study 20 revealed that there is not much free SeMet in human blood sample. RP or AE HPLC study for the hydrolysates of each selenoprotein that has been separated by double AF columns, which has not been reported before, could show in-depth information for selenoproteins in human blood serum.…”

mentioning

confidence: 95%

“…Thus, several researches [20][21][22][23] have been conducted to quantify these selenoproteins accurately in recent years.…”

mentioning

confidence: 99%

“…In this study, a combination of two AF columns is employed for the determination of GPx, SelP and SeAlb. Jitaru et al 20,[22][23][24] has been performing extensive researches on Se species in blood serum. They measured SeMet in blood serum with species specific ID to determine SeAlb concentration.…”

mentioning

confidence: 99%

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Quantitative Speciation of Selenium in Human Blood Serum and Urine with AE- RP- and AF-HPLC-ICP/MS

Jeong¹,

Lee²,

Pak

2013

Bulletin of the Korean Chemical Society

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Various separation modes in HPLC, such as anion exchange (AE), reversed-phase (RP), and affinity (AF) chromatography were examined for the separation of selenium species in human blood serum and urine. While RP-and AE-HPLC were mainly used for the separation of small molecular selenium species, double column AF-HPLC achieved the separation of selenoproteins in blood serum efficiently. Further, the effluent of AF-HPLC was enzymatically hydrolyzed and then analyzed with RP HPLC for selenoamino acid study. The versatility of the hybrid technique makes the in-depth study of selenium species possible. For quantification, post column isotope dilution (ID) with 78Se spike was performed. ORC ICP/MS (octapole reaction cell inductively coupled plasma/mass spectrometry) was used with 4 mL min −1 Hydrogen as reaction gas. In urine sample, inorganic selenium and SeCys were identified. In blood serum, selenoproteins GPx, SelP and SeAlb were detected and quantified. The concentration for GPx, SelP and SeAlb was 22.8 ± 3.4 ng g Enzymatic hydrolysis of each selenium proteins revealed that SeCys is the major amino acid for all three proteins and SeMet is contained in SeAlb only.

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supporting

confidence: 81%

mentioning

confidence: 95%

“…Thus, several researches [20][21][22][23] have been conducted to quantify these selenoproteins accurately in recent years.…”

mentioning

confidence: 99%

mentioning

confidence: 99%

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Quantitative Speciation of Selenium in Human Blood Serum and Urine with AE- RP- and AF-HPLC-ICP/MS

Jeong¹,

Lee²,

Pak

2013

Bulletin of the Korean Chemical Society

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“…An Se-containing protein is a protein that has Se incorporated into its peptide sequence as selenomethionine (SeMet), because animals are unable to discriminate SeMet from methionine (Met). 5 The most abundant Se-containing protein in human plasma is albumin. 6 However, some studies have indicated that no, or little, albumin is detected in the blood plasma of experimental animals, compared to human plasma.…”

Section: Introductionmentioning

confidence: 99%

Chromatographic Behavior of Selenoproteins in Rat Serum Detected by Inductively Coupled Plasma Mass Spectrometry

et al. 2013

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Hyphenated Methods for Speciation Analysis

Michalski

Jabłońska‐Czapla

Łyko

et al. 2000

Encyclopedia of Analytical Chemistry

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Speciation analysis, understood as research into various element forms, is gaining importance in environmental protection, biochemistry, geology, medicine, pharmacy, and food quality control. It is popular because what frequently determines the toxicological properties of a compound or element is not its total content. In many cases, it is the presence of its various forms. Studying low analyte concentrations, particularly in complex matrix samples, requires meticulous and sophisticated analytical techniques. The latest trends embrace the so‐called hyphenated methods, which combine different separation and detection methods. The following article describes the role and importance of hyphenated methods in speciation analyses of selected elements.

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A systematic approach to the accurate quantification of selenium in serum selenoalbumin by HPLC–ICP-MS

Cited by 73 publications

References 25 publications

Quantitative Speciation of Selenium in Human Blood Serum and Urine with AE- RP- and AF-HPLC-ICP/MS

Quantitative Speciation of Selenium in Human Blood Serum and Urine with AE- RP- and AF-HPLC-ICP/MS

Chromatographic Behavior of Selenoproteins in Rat Serum Detected by Inductively Coupled Plasma Mass Spectrometry

Hyphenated Methods for Speciation Analysis

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