A systematic approach to the development of fluorescent contrast agents for optical imaging of mouse cancer models

Kovar, Joy L.; Simpson, Melanie A.; Schutz-Geschwender, Amy; Olive, D. Michael

doi:10.1016/j.ab.2007.04.011

Cited by 141 publications

(143 citation statements)

References 59 publications

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“…The beauty of these techniques is that the cellular or molecular level of the target can be visualized and target location or texture can be identified with different dyes (Kovar et al, 2007;Massoud & Gambhir, 2003). Although OITs have limited imaging depth and procedure accessibility, they have become indispensable in many fields of research and clinical applications, including brain tumor research.…”

Section: Diagnostic Techniques and Surgical Management Of Brain Tumormentioning

confidence: 99%

Current Optical Imaging Techniques for Brain Tumor Research: Application of in vivo Laser Scanning Microscopy Imaging with a Cranial Window System

Chong¹,

Ku²,

Choi³

et al. 2011

Diagnostic Techniques and Surgical Management of Brain Tumors

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Section: Diagnostic Techniques and Surgical Management Of Brain Tumormentioning

confidence: 99%

Current Optical Imaging Techniques for Brain Tumor Research: Application of in vivo Laser Scanning Microscopy Imaging with a Cranial Window System

Chong¹,

Ku²,

Choi³

et al. 2011

Diagnostic Techniques and Surgical Management of Brain Tumors

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“…Optical imaging can be divided into bioluminescence and fluorescence. Optical imaging is based on the interaction of photons with tissue components and this interaction is characterized by three main criteria; light absorption, light scattering, and fluorescent emission [22].…”

Section: Optical Imagingmentioning

confidence: 99%

Imaging in animal models

Webb¹,

Yuan²,

Lemoine³

et al. 2016

Integr Cancer Sci Therap

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Advances in imaging techniques have significantly improved the choices available for in vivo imaging of animal research, particularly in the field of oncology. Development of existing technology has led to major advances in the miniaturisation of imaging techniques such as MRI, PET and CT enabling higher resolution imaging of smaller animals. One of the emerging growth areas is optical imaging, such as bioluminescence and fluorescence as a noninvasive technique to image orthotopic tumours models in mice. Recent developments in microscopic imaging techniques allow high resolution imaging of cancer cells in vivo in animal models. Another growth area is the development of multimodal scanners such as CT-PET to overcome individual limitations of a modality and to provide greater detail of tumours. This review discusses the strengths and limitations of established methods as well as more recent developments in preclinical imaging.

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“…NIR fluorescent dyes are not hindered by interfering autofluorescence because the patient's tissue generally exhibits negligible emission in this region. 1,4 Quantum Yield: Another criterion is that the dye should possess a high quantum yield. The fluorescence quantum yield (QY) reflects the efficiency of the fluorescence process.…”

Section: Fluorescent Dye Characteristicsmentioning

confidence: 99%

“…At this moment there are three major types of labels used in optical imaging: fluorescent proteins, bioluminescence, and fluorescent dyes. 1 Whereas bioluminescence and fluorescent proteins need engineering of cell lines or transgenic animals that carry the appropriate gene, fluorescent dyes do not, and may be unspecifically or specifically targeted to the relevant tissue. This property gives fluorescent dyes the potential for more rapid translation to clinical applications than approaches requiring genetic engineering.…”

Section: Introductionmentioning

confidence: 99%

The use of fluorescent dyes and probes in surgical oncology

Velde

Veerman

Subramaniam

et al. 2010

European Journal of Surgical Oncology (EJSO)

131

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Aims and background: Improved visualization of surgical targets inside of the patient helps to improve radical resection of the tumor while sparing healthy surrounding tissue. In order to achieve an image, optical contrast must be generated by properties intrinsic to the tissue, or require the attachment of special visualization labels to the tumor. In this overview the current status of the clinical use of fluorescent dyes and probes are reviewed. Methods:In this review, all experimental and clinical studies concerning fluorescent imaging were included. In addition, in the search for the optimal fluorescent imaging modality, all characteristics of a fluorescent dye were described. Findings and Conclusions:Although the technique of imaging through fluorescence sounds promising and several animal models show efficacy, official approval of these agents for further clinical evaluation, is eagerly awaited.

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A systematic approach to the development of fluorescent contrast agents for optical imaging of mouse cancer models

Cited by 141 publications

References 59 publications

Current Optical Imaging Techniques for Brain Tumor Research: Application of in vivo Laser Scanning Microscopy Imaging with a Cranial Window System

Current Optical Imaging Techniques for Brain Tumor Research: Application of in vivo Laser Scanning Microscopy Imaging with a Cranial Window System

Imaging in animal models

The use of fluorescent dyes and probes in surgical oncology

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