2018
DOI: 10.1007/978-1-4939-8814-3_7
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A Systematic Analysis Workflow for High-Density Customized Protein Microarrays in Biomarker Screening

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Cited by 4 publications
(6 citation statements)
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“…In this sense, comparing with mass spectroscopy used for the most abundant APRs, our novel method provided the possibility of exploring all the proteins in one single assay with low amount of sample and in a relative short time, testing more than 1000 analytes in just one array [22]. According to our previous experience in novel microarrays, we also have established a complete pipeline for development and analysis from the platform design to the final results useful for clinical outcome [23,30,31].…”
Section: Discussionmentioning
confidence: 99%
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“…In this sense, comparing with mass spectroscopy used for the most abundant APRs, our novel method provided the possibility of exploring all the proteins in one single assay with low amount of sample and in a relative short time, testing more than 1000 analytes in just one array [22]. According to our previous experience in novel microarrays, we also have established a complete pipeline for development and analysis from the platform design to the final results useful for clinical outcome [23,30,31].…”
Section: Discussionmentioning
confidence: 99%
“…The fluorescence signal retrieved from images processed in the previous section was corrected by subtracting background signal and then transformed into Z score as previously reported [30,31]. Overall raw fluorescence and log 2 (Z score) density distribution were compared to validate signal correction at each microarray employed.…”
Section: Aprs and Aab Microarray Data Pre-processing And Quality Controlmentioning
confidence: 99%
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“…The array images were analyzed by GenePix ® Pro 4.0 software ( Figure S2B ). In all of the arrays, the fluorescence signals were normalized (after background subtraction), as described by Valiente-Garcia R. et al (2019) [ 39 ] ( Figure S3 ), by a quartile normalization (based on all the arrays having the same homogeneous distribution).…”
Section: Methodsmentioning
confidence: 99%
“…The fluorescence signal retrieved from images processed in the previous section was corrected by subtracting background signal and then transformed into Z score as described in previous reports (26,27). Overall raw fluorescence and log 2 (Z score) density distribution were compared to validate signal correction at each microarray employed.…”
Section: Apps and Aab Microarray Data Pre-processing And Qcmentioning
confidence: 99%