A systematic analysis of the skeletal muscle miRNA transcriptome of chicken varieties with divergent skeletal muscle growth identifies novel miRNAs and differentially expressed miRNAs

Li, Tingting; Wu, Rimao; Zhang, Yong; Zhu, Dahai

doi:10.1186/1471-2164-12-186

Cited by 87 publications

(85 citation statements)

References 89 publications

(120 reference statements)

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“…Studies indicate that miR-130a is associated with lung cancer [50], gastric cancer [51,52], ovarian cancer [53][54][55][56][57], cervical cancer [58,59], and prostate cancer [60]. Recently, deep sequencing technology was used to identify differentially expressed host miRNAs in chickens [28,34,35,61,62]. Burnside et al reported that ggamiR-130a expression was lower in MDV-infected samples [28], a finding that is consistent with results from a previous study by our team [34].…”

Section: Discussionsupporting

confidence: 90%

Chicken gga-miR-130a targets HOXA3 and MDFIC and inhibits Marek’s disease lymphoma cell proliferation and migration

Han

Lian

et al. 2016

Mol Biol Rep

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Marek's disease (MD) is an infectious disease of chickens caused by MD virus (MDV), which is a herpesvirus that initiates tumor formation. Studies have indicated that microRNAs (miRNAs) are linked with the development of cancers or tumors. Previously, gga-miR-130a was discovered downregulated in MDV-infected tissues. Here, we aimed to explore the further function of gga-miR-130a in MD. The expression of gga-miR-130a in MDV-infected and uninfected spleens was detected by quantitative real-time PCR (qRT-PCR). Subsequently, proliferation and migration assays of MDV-transformed lymphoid cells (MSB1) were carried out by transfecting gga-miR-130a. The target genes of gga-miR-130a were predicted using TargetScan and miRDB and clustered through Gene Ontology analysis. The target genes were validated by western blot, qRT-PCR, and a dual luciferase reporter assay. Our results show that the expression of gga-miR-130a was reduced in MDV-infected spleens. Gga-miR-130a showed an inhibitory effect on MSB1 cell proliferation and migration. Two target genes, homeobox A3 (HOXA3) and MyoD family inhibitor domain containing (MDFIC), were predicted and clustered to cell proliferation. Results indicate that gga-miR-130a regulates HOXA3 and MDFIC at the protein level but not at the mRNA level. Moreover, the gga-miR-130a binding sites of two target genes have been confirmed. We conclude that gga-miR-130a can arrest MSB1 cell proliferation and migration, and target HOXA3 and MDFIC, which are both involved in the regulation of cell proliferation. Collectively, gga-miR-130a plays a critical role in the tumorigenesis associated with chicken MD.

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Section: Discussionsupporting

confidence: 90%

Chicken gga-miR-130a targets HOXA3 and MDFIC and inhibits Marek’s disease lymphoma cell proliferation and migration

Han

Lian

et al. 2016

Mol Biol Rep

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“…3). Our data show similar results as reported previously: the miRNA expression patterns in broilers and layers were investigated in skeletal muscles, and let-7c, miR-15b, miR-15c, and miR-2188 were differentially expressed [15]. In addition, miR-133a, miR-181a, and miR-206 were also muscle specific in mouse, pig, beef cattle, and other species [11,24].…”

Section: Discussionsupporting

confidence: 78%

“…According to the homology of the species, we boldly speculate that these miRNAs are also involved in the skeletal muscle differentiation in broilers. Recently, numerous miRNAs have been discovered in various chicken organs and tissues, such as embryo, somite, pre-adipocyte, and skeletal muscle [12,13,14,15], but the miRNA expression profiles of the broiler skeletal muscle awaits further study.…”

Section: Introductionmentioning

confidence: 99%

Solexa Sequencing of MicroRNAs on Chromium Metabolism in Broiler Chicks

Pan

Dai

et al. 2013

Lifestyle Genomics

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Aim: The aim of this study was to determine the effect of chromium picolinate (CrPic) on the differential expression of the known microRNAs (miRNAs) in broiler skeletal muscle. Methods and Results: A total of 288 1-day-old male Arbor Acres broilers were randomly assigned to one of four dietary treatments supplemented with 0, 0.4, 2.0, or 10.0 mg·kg^-1 CrPic, respectively. Dietary CrPic supplementation at 10.0 mg·kg^-1 increased the average daily feed intake in broilers (p < 0.05). On day 42, the serum total protein level was highest in animals treated with 2.0 mg·kg^-1 (p < 0.05) and 10.0 mg·kg^-1 CrPic (p < 0.05). Dietary supplementation with 10.0 mg·kg^-1 CrPic decreased the levels of serum glucose (p < 0.05) on day 42 and of serum triglyceride (p < 0.05) on days 21 and 42. To further identify miRNAs from broiler skeletal muscles, we sequenced two small RNA libraries using the Solexa sequencing approach, and 57 miRNAs were found to be significantly differentially expressed (p < 0.05). Among them, 6 upregulated and 2 downregulated miRNAs were validated by real-time qPCR (p < 0.05). Conclusions: The results of the present study provide a valuable clue regarding the role of miRNA target genes in the mechanism of the dietary CrPic effect on protein synthesis in skeletal muscles of broilers.

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“…Using the same cloning strategy, Wang et al identified 47 miRNAs from chicken adipose tissue and skeletal muscle (Wang et al, 2012). Using deep sequencing, 123 miRNAs were discovered from embryonic chick livers at embryonic days 15 (E15) and E20 , 121 known chicken miRNAs, 449 new chicken miRNAs and 39 mirtrons were identified from E5, E7 and E9 chicken embryos (Glazov et al, 2008), and 63 and 33 novel chicken miRNAs were identified from chicken preadipocytes and skeletal muscle, respectively (Li et al, 2011c;Yao et al, 2011). Microarrays were used to profile the expression of chicken miRNAs at different stages of gonadal sex differentiation.…”

Section: Mirnas In Chickensmentioning

confidence: 99%

“…Li and colleagues further identified the activin receptor 2B (ACVR2B) gene as a miR-1 target in chicken muscle (Li et al, 2011c).…”

Section: Mirnas In Chickensmentioning

confidence: 99%

MicroRNAs in farm animals

Wang

Jiang

2013

Animal

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MicroRNAs (miRNAs) are a class of ,22 nucleotide-long small noncoding RNAs that target mRNAs for translational repression or degradation. miRNAs target mRNAs by base-pairing with the 3 0 -untranslated regions (3 0 -UTRs) of mRNAs. miRNAs are present in various species, from animals to plants. In this review, we summarize the identification, expression, and function of miRNAs in four important farm animal species: cattle, chicken, pig and sheep. In each of these species, hundreds of miRNAs have been identified through homology search, small RNA cloning and next generation sequencing. Real-time RT-PCR and microarray experiments reveal that many miRNAs are expressed in a tissue-specific or spatiotemporal-specific manner in farm animals. Limited functional studies suggest that miRNAs have important roles in muscle development and hypertrophy, adipose tissue growth, oocyte maturation and early embryonic development in farm animals. Increasing evidence suggests that single-nucleotide polymorphisms in miRNA target sites or miRNA gene promoters may contribute to variation in production or health traits in farm animals.

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A systematic analysis of the skeletal muscle miRNA transcriptome of chicken varieties with divergent skeletal muscle growth identifies novel miRNAs and differentially expressed miRNAs

Cited by 87 publications

References 89 publications

Chicken gga-miR-130a targets HOXA3 and MDFIC and inhibits Marek’s disease lymphoma cell proliferation and migration

Chicken gga-miR-130a targets HOXA3 and MDFIC and inhibits Marek’s disease lymphoma cell proliferation and migration

Solexa Sequencing of MicroRNAs on Chromium Metabolism in Broiler Chicks

MicroRNAs in farm animals

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