A Systematic Analysis of Drosophila Gustatory Receptor Gene Expression in Abdominal Neurons which Project to the Central Nervous System

Park, Jeong-Ho; Kwon, Jae Young

doi:10.1007/s10059-011-0128-1

Cited by 53 publications

(46 citation statements)

References 48 publications

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“…Several Gr-GAL4 drivers have been shown to be expressed in similar neurons, although no taste function has been ascribed to them (Park and Kwon, 2011). …”

Section: Resultsmentioning

confidence: 99%

The Drosophila IR20a Clade of Ionotropic Receptors Are Candidate Taste and Pheromone Receptors

Koh

Gorur-Shandilya

et al. 2014

Neuron

276

335

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Insects use taste to evaluate food, hosts, and mates. Drosophila has many “orphan” taste neurons that express no known taste receptors. The Ionotropic Receptor (IR) superfamily is best known for its role in olfaction, but virtually nothing is known about a clade of ~35 members, the IR20a clade. Here, a comprehensive analysis of this clade reveals expression in all taste organs of the fly. Some members are expressed in orphan taste neurons, whereas others are coexpressed with bitter- or sugar-sensing Gustatory receptor (Gr) genes. Analysis of the closely related IR52c and IR52d genes reveals signatures of adaptive evolution, roles in male mating behavior, and sexually dimorphic expression in neurons of the male foreleg, which contacts females during courtship. These neurons are activated by conspecific females and contact a neural circuit for sexual behavior. Together, these results greatly expand the repertoire of candidate taste and pheromone receptors in the fly.

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“…Several Gr-GAL4 drivers have been shown to be expressed in similar neurons, although no taste function has been ascribed to them (Park and Kwon, 2011). …”

Section: Resultsmentioning

confidence: 99%

The Drosophila IR20a Clade of Ionotropic Receptors Are Candidate Taste and Pheromone Receptors

Koh

Gorur-Shandilya

et al. 2014

Neuron

276

335

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“…The UAS-CG9918RNAi line (TRiP #27539, Bloomington Stock Center) has been described 48 . Gr36C -Gal4, Myo1A ts -Gal4, voila -Gal4, and S106 -Gal4 have been described 49–51 (FlyBase). Myo1A -Gal4 is specifically expressed in enterocytes 52 .…”

Section: Methodsmentioning

confidence: 99%

An obligatory role for neurotensin in high-fat-diet-induced obesity

Song

Zaytseva

et al. 2016

Nature

219

237

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Obesity and its associated comorbidities (e.g., diabetes mellitus and hepatic steatosis) contribute to approximately 2.5 million deaths annually1 and are among the most prevalent and challenging conditions confronting the medical profession2,3. Neurotensin (NT), a 13-amino acid peptide predominantly localized in specialized enteroendocrine (EE) cells of the small bowel4 and released by fat ingestion5, facilitates fatty acid (FA) translocation in rat intestine6, and stimulates growth of various cancers7; the effects of NT are mediated through three known NT receptors (NTR1, 2 and 3)8. Increased fasting plasma levels of pro-NT (a stable NT precursor fragment produced in equimolar amounts relative to NT) are associated with increased risk of diabetes, cardiovascular disease and mortality9; however, a role for NT as a causative factor in these diseases is unknown. Here, we show that NT-deficient mice demonstrate significantly reduced intestinal fat absorption and are protected from obesity, hepatic steatosis and insulin resistance associated with high fat consumption. We further demonstrate that NT attenuates the activation of AMP-activated protein kinase (AMPK) and stimulates FA absorption in mice and in cultured intestinal cells, and that this occurs through a mechanism involving NTR1 and NTR3/sortilin. Consistent with the findings in mice, expression of NT in Drosophila midgut EE cells results in increased lipid accumulation in the midgut, fat body, and oenocytes (specialized hepatocyte-like cells) and decreased AMPK activation. Remarkably, in humans, we show that both obese and insulin-resistant subjects have elevated plasma concentrations of pro-NT, and in longitudinal studies among non-obese subjects, high levels of pro-NT denote a doubling of the risk of developing obesity later in life. Our findings directly link NT with increased fat absorption and obesity and suggest that NT may provide a prognostic marker of future obesity and a potential target for prevention and treatment.

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“…For the immunohistochemistry of the brain and ventral nerve cord (VNC), adult brains and VNC were dissected and prepared as described [21,23]. Samples were incubated with primary antibodies or antiserum for 48 h at 4 • C, washed with PBS-T, and incubated with secondary antibodies overnight at 4 • C.…”

Section: Immunostainingmentioning

confidence: 99%

Isoform-specific expression of the neuropeptide orcokinin in Drosophila melanogaster

Choi

Mizoguchi

et al. 2015

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A Systematic Analysis of Drosophila Gustatory Receptor Gene Expression in Abdominal Neurons which Project to the Central Nervous System

Cited by 53 publications

References 48 publications

The Drosophila IR20a Clade of Ionotropic Receptors Are Candidate Taste and Pheromone Receptors

The Drosophila IR20a Clade of Ionotropic Receptors Are Candidate Taste and Pheromone Receptors

An obligatory role for neurotensin in high-fat-diet-induced obesity

Isoform-specific expression of the neuropeptide orcokinin in Drosophila melanogaster

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