A system for tumor heterogeneity evaluation and diagnosis based on tumor markers measured routinely in the laboratory

Liu, Hui; Rixv, Liu; Zhou, Xifa

doi:10.1016/j.clinbiochem.2015.07.027

Cited by 12 publications

(10 citation statements)

References 37 publications

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“…Moreover, the abundant capillaries were mainly located in the periphery of the tumors. At present, there is no specific tumor marker for gastric cancer; however, CA199, CK-7, and CDX-2 are commonly used as markers of gastrointestinal tumors [21][22][23]. Thus, we chose these three markers for the labeling and identification of human-derived gastric cancer cells.…”

Section: Discussionmentioning

confidence: 99%

Establishment of a Human Gastric Cancer Xenograft Model in Immunocompetent Mice Using the Microcarrier-6

Wang

Yang

et al. 2020

BioMed Research International

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Gastric cancer is among the most common malignant tumors of the digestive tract. Establishing a robust and reliable animal model is the foundation for studying the pathogenesis of cancer. The present study established a mouse model of gastric carcinoma by inoculating immunocompetent mice with MKN45 cells using microcarrier. Sixty male C57BL/6 mice were randomly divided into three groups: a 2D group, an empty carrier group, and a 3D group, according to the coculture system of MKN45 and the microcarrier. The mouse models were established by hypodermic injection. Time to develop tumor, rate of tumor formation, and pathological features were observed in each group. In the 3D group, the tumorigenesis time was short, while the rate of tumor formation was high (75%). There was no detectable tumor formation in either the 2D or the empty carrier group. Both H&E and immunohistochemical staining of the tumor xenograft showed characteristic evidence of human gastric neoplasms. The present study successfully established a human gastric carcinoma model in immunocompetent mice, which provides a novel and valuable animal model for the cancer research and development of anticancer drugs.

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Section: Discussionmentioning

confidence: 99%

Establishment of a Human Gastric Cancer Xenograft Model in Immunocompetent Mice Using the Microcarrier-6

Wang

Yang

et al. 2020

BioMed Research International

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“…The receiver operating characteristic (ROC) analysis is the common method used to evaluate the effectiveness of diagnosis made using a biomarker [1,2,12]. In present study, ROC analysis was evaluated based on CIB whether the ROC analysis was still available or not.…”

Section: Evaluation Of Roc Analysismentioning

confidence: 99%

“…One of the main purposes of seeking biomarkers is diagnosis of a disease. However, most studies to identify biomarkers use case-control studies rather than cohort studies [1][2][3]. In case-control studies, the potential relationship between a biomarker and the disease is examined by comparing frequencies of this biomarker in the diseased and non-diseased subjects.…”

Section: Introductionmentioning

confidence: 99%

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An Comprehensive Index (CIB) with Combination of Consistency in both Case Control and Cohort Study to Determine the Efficacy of a Biomarker

Liu¹

2020

Preprint

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Background: This study aimed to describe biomarkers using the comprehensive index of biomarker (CIB) based on consistency rate in both case control (Youden index, Yen) and cohort study (Crc) to determine the efficacy of a biomarker. Methods: The CIB is the geometric mean of Yen and Crc. The simulated data were generated to observe CIB features of sensitivity, specificity, and receiver operating characteristic (ROC) analysis for biomarkersResults: CIB was found to be related to the CRC values ROC analysis. The higher Spe could indicate better diagnostic power and the higher Sen could indicate better joint action for biomarkers with the same Yen. Although Yen is the common index used to evaluate the effectiveness of a biomarker, unfortunately, the Yen value was significantly larger than CIB value under the moderate Spe, showing overestimation.Conclusion: The CIB with combination of consistency in both case control and cohort study could be more reasonable. The CIB could provide a better understanding of the power of a biomarker and would be better at evaluating biomarkers from new systems or concepts.

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“…Quantitative variations in a particular event are normally described in terms of changes in ratio and absolute values, although these factors are not comparable. For example, resolving power of biomarker is normally described in terms of changes in ratio (odds ratio) [1][2][3] and absolute values (Youden index) [4][5][6]. When the cardinal number (value in control group) is relatively small, the increase in ratio may be very high although the absolute increase may be not high.…”

Section: Introductionmentioning

confidence: 99%

An Angle Compared Index with Hybrid of Changes in the Ratio and Amplitude for Quantitative Evaluation of Disease Risk, Biological Function, and Biomarker Efficacy

Jin

Liu

2019

BioMed Research International

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Objective. The purpose of this study was to describe variations of different cardinal frequency by using angle compared index (ACI). Methods. The basic principle of the analysis model is to comprehensively consider changes in both the ratio and absolute value as follows: ACI = arctan Pd⁎(1-Pc) / [Pc⁎(1-Pd)] + arctan (Pd - Pc) - 45, where Pd represent percentage of disease occurrence in disease group and Pc represent that in control group. The range of ACI was (0~90). Thus, ACIs from different cardinal frequency are comparable. Results. When biomarkers with similar ratio value, absolute value, or ACI but different positive frequencies were combined, although three indexes (ratio value, absolute value, or ACI) increased after two single biomarkers were combined, only ACI increased with similar amplitudes after two single biomarkers with the same ACI at different positive frequencies were combined. Conclusion. The ACI provides a better understanding power of biomarker and may be a relatively good index for evaluating the complex events represented by different cardinal frequency from new systems.

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A system for tumor heterogeneity evaluation and diagnosis based on tumor markers measured routinely in the laboratory

Cited by 12 publications

References 37 publications

Establishment of a Human Gastric Cancer Xenograft Model in Immunocompetent Mice Using the Microcarrier-6

Establishment of a Human Gastric Cancer Xenograft Model in Immunocompetent Mice Using the Microcarrier-6

An Comprehensive Index (CIB) with Combination of Consistency in both Case Control and Cohort Study to Determine the Efficacy of a Biomarker

An Angle Compared Index with Hybrid of Changes in the Ratio and Amplitude for Quantitative Evaluation of Disease Risk, Biological Function, and Biomarker Efficacy

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