A system for large scale production of chrysanthemum using microponics with the supplement of silver nanoparticles under light-emitting diodes

Tùng, Hoàng Thanh; Nam, Nguyễn Bá; Huy, Nguyễn Xuân; Luận, Vũ Quốc; Hiền, Vũ Thị; Phuong, Truong Thi Bich; Le, Dung Tien; Lộc, Nguyễn Hoàng; Nhựt, Dương Tấn

doi:10.1016/j.scienta.2017.12.063

Cited by 30 publications

(21 citation statements)

References 25 publications

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“…Phytostimulatory effects of biosynthesized AgNPs on seedling elongation and their biomass growth have been reported in Oryza sativa [17] and Trigonella foenum-graceum [16]. Treatment of Chrysanthemum morifolium with 7.5 ppm AgNPs applied to tissue cultures significantly improved plant height, length and width of leaves, and plant fresh and dry weight [36]. In Swertia chirata, supplementation of the cultivation media with AgNPs of 20 nm in diameter favorably influenced shoot morphogenesis [7].…”

Section: Discussionmentioning

confidence: 95%

Stimulatory Effect of Silver Nanoparticles on the Growth and Flowering of Potted Oriental Lilies

et al. 2019

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Nanoparticles exhibit unique biological activities and may serve as novel plant growth stimulators. This research consisted of a two-year pot experiment designed to find out if silver nanoparticles (AgNPs) might be used in the cultivation of Oriental lilies. In the first year, we evaluated the effects of various concentrations of AgNPs (0, 25, 50, 100, and 150 ppm) and their application methods (pre-planting bulb soaks, foliar sprays, and substrate drenches) on the growth and flowering of Lilium cv. Mona Lisa. In the second year, we evaluated the effects of soaking the bulbs of cv. Little John in the same concentration of AgNP solution on plant morphological features, leaf content of photosynthetic pigments, basic macronutrients, and complex biomolecules with the use of the Fourier-transform infrared spectroscopy (FTIR). Soaking the bulbs in a nanoparticle solution turned out to be the most effective strategy for growth and flowering promotion. AgNPs stimulated plant growth, as manifested by enhanced accumulation of leaf and bulb biomass and accelerated flowering. Moreover, plants treated with silver nanoparticles showed higher leaf greenness index, formed more flowers, and flowered longer. At 100 ppm AgNPs, the leaves accumulated the highest content of chlorophyll a, chlorophyll b, and carotenoids, and were the richest in potassium, calcium, and sulfur. The FTIR spectra did not show any changes in absorbance intensity and chemical composition in the leaves from AgNP-treated bulbs.

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Section: Discussionmentioning

confidence: 95%

Stimulatory Effect of Silver Nanoparticles on the Growth and Flowering of Potted Oriental Lilies

et al. 2019

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“…Research on resistance to many microorganisms of AgNPs in culture is still very limited, so this is a prerequisite study to add more data on the microorganism resistance of AgNPs. Tung et al (2018) used AgNPs in micoponic culture, the results showed that AgNPs plays a role in increasing plant growth as well as reducing a number of microorganisms (eight bacterial species and three fungal species) in the microponic medium. Disinfection of ex vitro leaves, flower stalks and stems with HgCl2 disinfectant, the recorded results show that the explant induction only reach about 30 -35% (Nhut et al, 2005).…”

Section: Embryogenic Callus Formationmentioning

confidence: 99%

Silver nanoparticles enhanced efficiency of explant surface disinfection and somatic embryogenesis in Begonia tuberous via thin cell layer culture

Tùng¹,

Van²,

Bao³

et al. 2021

Vietnam J. Biotechnol.

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In vitro culture establishment is one of the most important stages in micropropagation. The disinfectant effectiveness depends on the type of surface disinfectant, concentration and the time treatment. In this initial study, silver nanoparticles (AgNPs) were used as a disinfectant for petioles, flower stalks and stems of Begonia tuberous. In addition, thin cell layer culture (TCL) technique has been applied for the purpose of somatic embryogenesis. The results showed that AgNPs were effective in eliminating infectious microorganisms on B. tuberous explants; which were identified included 4 species of fungi (Fusarium sp., Aspergillus aculeatus, Trichoderma sp. and Penicillium sp.) and 1 species of bacteria (Pseudomonas sp.). At concentrations of 200 ppm and 300 ppm, AgNPs were not only effective in disinfection but also increased the induction rate of somatic embryogenesis in flower stalk TCL explants (approximately 40.00%); a similar effect was observed in stem TCL explants at the same concentration. Meanwhile, for petiole TCL explants, the induction rate of somatic embryogenesis was optimal when using AgNPs at a concentration of 100 - 300 ppm to disinfected the explant. In contrast, at high (400 ppm) or low (50 ppm) concentrations of AgNPs did not play a disinfecting role and stimulated somatic embryogenesis. In addition, explants derived from AgNPs sterilization did not show any abnormalities in somatic embryogenesis with shapes such as globular, heart, torpedo, and cotyledon. AgNPs showed double efficacy in sterilization of explants and improved efficiency of somatic embryogenesis from TCL petioles, flower stalks and stems explants; thus increasing the efficiency micropropagation of B. tuberous.

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“…Microponic culture: A circular plastic box (500 mL) with 8.5 cm in height, 12 cm in top diameter and 9 cm in bottom diameter (Dai Dong Tien, Vietnam) was contained 40 mL of ½ MS medium (a half mineral) (Tung et al, 2018).…”

Section: Effect Of Fenps On the Growth Of Chrysanthemum In Culture Symentioning

confidence: 99%

“…So far, studies on microroponic have been limited. Microponic systems have many advantages over traditional breeding systems because of inheriting the advantages of micropropagation and culture systems (Hahn et al, 1996(Hahn et al, , 1998(Hahn et al, , 2000Nhut et al, 2005;Tung et al, 2018). In a microponic system, it only takes 2 weeks for the plant to transfer the plants to the greenhouse.…”

Section: Microponic Culturementioning

confidence: 99%

Iron nanoparticles on growth and acclimatization of Chrysanthemum morifolium Ramat. cv. "Jimba" in different culture systems

Tùng¹,

Phong²,

Nguyen³

et al. 2020

Vietnam J. Biotechnol.

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In plant tissue culture, iron nanoparticles (FeNPs) was one of the first types of nano to be used in plants. Previous reports have identified the effect of FeNPs on many different plant species. In this study, FeNPs was used to replace Fe-EDTA in MS (Murashige, Skoog, 1962) medium to assess their effects on growth, chlorophyll (a, b and a+b) accumulation, antioxidant activity of ascorbate peroxidase (APX) and superoxide dismutase (SOD) enzymes, and acclimatization in greenhouse conditions in different culture systems (in vitro solid, in vitro hydroponic and microponic culture). The obtained results show that FeNPs added to MS medium was higher growth, chlorophyll (a, b and a+b) content, antioxidant activity of SOD and APX enzymes than Fe-EDTA in MS medium as control treatment. The effect of FeNPs are differences between culture systems. In vitro solid and microponic culture systems, the optimal concentration is 75 mM FeNPs and in vitro hydroponic culture system is 100 mM FeNPs. The optimal activity of the antioxidant enzyme SOD (35.04 U.mg−1 prot) obtained in the roots of cultured plants in microponic culture system; meanwhile, the optimal activity of the antioxidant enzyme APX (2.11 μmol.min−1.mg−1 prot) obtained in leaves cultivated in solid culture system. The plantlets derived from MS medium added FeNPs were transfered into greenhouse conditions, the microponic cultivated plants supplemented with FeNPs at a concentration of 100 mM gave the highest survival rate (94.67%). The results of this study showed that FeNPs can replace Fe-EDTA salt in MS medium, and iron deficiency in culture media will reduce chlorophyll content.

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A system for large scale production of chrysanthemum using microponics with the supplement of silver nanoparticles under light-emitting diodes

Cited by 30 publications

References 25 publications

Stimulatory Effect of Silver Nanoparticles on the Growth and Flowering of Potted Oriental Lilies

Stimulatory Effect of Silver Nanoparticles on the Growth and Flowering of Potted Oriental Lilies

Silver nanoparticles enhanced efficiency of explant surface disinfection and somatic embryogenesis in Begonia tuberous via thin cell layer culture

Iron nanoparticles on growth and acclimatization of Chrysanthemum morifolium Ramat. cv. "Jimba" in different culture systems

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