A synthetic peptide containing loop 4 of nerve growth factor for targeted gene delivery

Zeng, Jieming; Too, Heng‐Phon; Ma, Yuexia; Luo, Elizabeth S. E.; Wang, Shu

doi:10.1002/jgm.610

Cited by 24 publications

(10 citation statements)

References 56 publications

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“…Several strategies have been employed to overcome this challenge in the non‐viral gene delivery field, one of which is to utilize materials that specifically recognize neurons. Neuron‐specific toxin fragments, neurotransmitters and nerve growth factors have been employed as mediators of neuron recognition 13, 14, 16, 31. In this study, two materials, Tet1‐PEI and NT‐PEI, are evaluated for neuron‐specific gene delivery.…”

Section: Discussionmentioning

confidence: 99%

Neuron‐specific delivery of nucleic acids mediated by Tet1‐modified poly(ethylenimine)

Park

Lašienė

Chou

et al. 2007

The Journal of Gene Medicine

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Background-The development of minimally invasive, non-viral gene delivery vehicles for the central nervous system (CNS) is an important technology goal in the advancement of molecular therapies for neurological diseases. One approach is to deliver materials peripherally that are recognized and retrogradely transported by motor neurons toward the CNS. Tet1 is a peptide identified by Boulis and coworkers to possess the binding characteristics of tetanus toxin, which interacts specifically with motor neurons and undergoes fast, retrograde delivery to cell soma. In this work, Tet1-poly(ethylenimine) (Tet1-PEI) was synthesized and evaluated as a neurontargeted delivery vehicle.

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Section: Discussionmentioning

confidence: 99%

Neuron‐specific delivery of nucleic acids mediated by Tet1‐modified poly(ethylenimine)

Park

Lašienė

Chou

et al. 2007

The Journal of Gene Medicine

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“…However, gene delivery to the CNS using nonviral vectors has not been successful due to the low transfection efficiencies achieved in non-dividing cells and low cellular uptake rates in terminally-differentiated neurons [88-90]. One approach to increase efficacy is to conjugate targeting ligands to polymeric vectors to enhance uptake by neuronal subtypes [7,91,92]. We previously demonstrated that conjugation of Tet1, a 12-mer peptide identified by in vitro phage display to bind the Gtb1 ganglioside [93], to polyethylenimine (PEI) enhanced gene delivery to neural progenitor cells (NPCs) [94].…”

Section: Design Of Peptide-based Polymersmentioning

confidence: 99%

Engineering biodegradable and multifunctional peptide-based polymers for gene delivery

2013

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The complex nature of in vivo gene transfer establishes the need for multifunctional delivery vectors capable of meeting these challenges. An additional consideration for clinical translation of synthetic delivery formulations is reproducibility and scale-up of materials. In this review, we summarize our work over the last five years in developing a modular approach for synthesizing peptide-based polymers. In these materials, bioactive peptides that address various barriers to gene delivery are copolymerized with a hydrophilic backbone of N-(2-hydroxypropyl)methacrylamide (HPMA) using reversible-addition fragmentation chain-transfer (RAFT) polymerization. We demonstrate that this synthetic approach results in well-defined, narrowly-disperse polymers with controllable composition and molecular weight. To date, we have investigated the effectiveness of various bioactive peptides for DNA condensation, endosomal escape, cell targeting, and degradability on gene transfer, as well as the impact of multivalency and polymer architecture on peptide bioactivity.

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“…Many peptides have been described in the literature as receptor-specific ligands so any of them can be added to the multifunctional proteins in order to confer them cell specificity. [47][48][49][50][51][52][53][54][55][56][57][58][59][60][61][62] The most natural specific ligands that can also be used for cell targeting are monoclonal antibodies. 32,[63][64][65] In addition, if no specific peptides are available for an intended target, new specific binding peptides can be found by using phage display 66 or combinatorial chemistry.…”

Section: Cell Binding and Internalizationmentioning

confidence: 99%

Engineered Biological Entities for Drug Delivery and Gene Therapy

Domingo-Espín

Unzueta

Saccardo

et al. 2011

Progress in Molecular Biology and Translational Science

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The development of genetic engineering techniques has speeded up the growth of the biotechnological industry, resulting in a significant increase in the number of recombinant protein products on the market. The deep knowledge of protein function, structure, biological interactions, and the possibility to design new polypeptides with desired biological activities have been the main factors involved in the increase of intensive research and preclinical and clinical approaches. Consequently, new biological entities with added value for innovative medicines such as increased stability, improved targeting, and reduced toxicity, among others have been obtained. Proteins are complex nanoparticles with sizes ranging from a few nanometers to a few hundred nanometers when complex supramolecular interactions occur, as for example, in viral capsids. However, even though protein production is a delicate process that imposes the use of sophisticated analytical methods and negative secondary effects have been detected in some cases as immune and inflammatory reactions, the great potential of biodegradable and tunable protein nanoparticles indicates that protein-based biotechnological products are expected to increase in the years to come.

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A synthetic peptide containing loop 4 of nerve growth factor for targeted gene delivery

Abstract: The chimeric peptide reported will be useful in gene delivery to and gene therapy of the nervous system and other tissues/organs with cells expressing TrkA.

Cited by 24 publications

References 56 publications

Neuron‐specific delivery of nucleic acids mediated by Tet1‐modified poly(ethylenimine)

Neuron‐specific delivery of nucleic acids mediated by Tet1‐modified poly(ethylenimine)

Engineering biodegradable and multifunctional peptide-based polymers for gene delivery

Engineered Biological Entities for Drug Delivery and Gene Therapy

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