A Synthetic Pathway for the Production of Benzylsuccinate in <em>Escherichia coli</em>
Johanna Mock,
Karola Schühle,
Uwe Linne
et al.
Abstract:Benzylsuccinate is generated in anaerobic toluene degradation by radical addition of toluene to fumarate and further degraded to benzoyl-CoA by a beta-oxidation pathway. Using metabolic modules for benzoate transport and activation to benzoyl-CoA and the enzymes of benzylsuccinate beta-oxidation, we established an artificial pathway for benzylsuccinate production in Escherichia coli, which is based of its degradation pathway running in reverse. Benzoate is supplied to the medium, but needs to be converted to b… Show more
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