A synthetic metabolic network for physicochemical homeostasis

Pols, Tjeerd; Sikkema, Hendrik R; Gaastra, Bauke F; Frallicciardi, Jacopo; Śmigiel, Wojciech M.; Singh, Shubham; Poolman, Bert

doi:10.1038/s41467-019-12287-2

Cited by 58 publications

(113 citation statements)

References 53 publications

(68 reference statements)

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“…Second, such a production would correspond to the emancipation from the current "windup toy" approaches based on endowing SCs, at time zero, with all required chemical energy to run, and the consequent ceasing when such supply runs out. Moreover, when the two cuncurrent processes of ATP production and usage are coupled, the system nicely constitutes a realization of out-of-equilibrium homeostasis (Pols et al, 2019).…”

Section: Membrane Proteins As Key Elements For Vectorial Chemistry In Single Compartment-and Nested Multicompartment-synthetic Cellsmentioning

confidence: 99%

The Rise of the Nested Multicompartment Model in Synthetic Cell Research

Altamura

Albanese

Mavelli

et al. 2021

Front. Mol. Biosci.

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The attractiveness of the "bottom-up" approach as a viable route for constructing cell-like systems (Luisi, 2002;Stano, 2019) is clearly evident by the ever increasing number of international projects and initiatives dedicated to this fascinating research (Supplementary Text S1). Such cell-like systems, simply called "synthetic cells" (SCs), "artificial cells" or "protocells" (although with slighly different nuances of meaning) are compartment-based systems (often, but not only, liposomes), capable of mimicking some aspects of cell behavior in a range of manners, and can be variously conceived in terms of materials, designs, and scopes. Even if current SCs are not alive, there is a recognized optimism among practicioners about the contribution of this research to basic and applied science, and there is the bet it will become one of the most important biotechnologies in the near future,-not resembling anything existing before-for example in nanomedicine (

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Section: Membrane Proteins As Key Elements For Vectorial Chemistry In Single Compartment-and Nested Multicompartment-synthetic Cellsmentioning

confidence: 99%

The Rise of the Nested Multicompartment Model in Synthetic Cell Research

Altamura

Albanese

Mavelli

et al. 2021

Front. Mol. Biosci.

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“…El laboratorio de Bert Poolman ha demostrado que el uso de proteínas de membrana insertadas en la capa doble de fosfolípidos, como el antiportador arginina/ornitina, que facilita la entrada de arginina (sustrato) en la célula y la salida de la ornitina (producto) en el exterior, garantiza la producción de ATP y mantiene lejos del equilibrio, garantizando así la energía para sostener la vida (Pols et al, 2019). En la célula, de hecho, la conversión de la arginina en ornitina, amoníaco y dióxido de carbono produce ATP en tres pasos enzimáticos.…”

Section: Los Avances Hasta La Fechaunclassified

Ciencia ficción de hoy, ciencia de mañana: construir una vida

Presutti

2021

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Cuando la ciencia ficción, el arte de la imaginación, se encuentra con la ciencia, se pueden crear cosas que antes parecían inimaginables. Es lo que sucede combinando fantasía y técnica, que hacen realidad proyectos imaginados y a veces sólo narrados en novelas, series de televisión o películas. Uno de los grandes sueños de los biólogos, de hecho, ya aparecido en una serie de televisión de ciencia ficción, es poder construir una vida, para poder entender no sólo cómo funciona sino también para tener posibles intuiciones sobre la vida en otros planetas. Este es el caso de un proyecto ambicioso en el que, utilizando los componentes moleculares de la biología, sería posible construir una célula sintética. En este artículo, partiendo del concepto de ciencia ficción y llegando a la conciencia de la sinergia que existe entre ciencia ficción y ciencia, se describirán algunos de los avances realizados hasta la fecha en la construcción de una vida celular, un tema que no sólo forma parte de la literatura científica ficción, sino que gracias a los avances científicos se está convirtiendo cada vez màs en realidad.

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“…Both functional transient vesicles, [11] and thermodynamically stable vesicles exhibiting chemically fuelled functions have been explored [12] . Alternatively, vesicles involving intricate reaction networks [13] that can be compartmentalized enable functionality such as movement [14] …”

Section: Figurementioning

confidence: 99%

Coupled Metabolic Cycles Allow Out‐of‐Equilibrium Autopoietic Vesicle Replication

et al. 2020

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We report chemically fuelled out-of-equilibrium selfreplicating vesicles based on surfactant formation. We studied the vesicles autocatalytic formation using UPLC to determine monomer concentration and interferometric scattering microscopy at the nanoparticle level. Unlike related reports of chemically fuelled self-replicating micelles, our vesicular system was too stable to surfactant degradation to be maintained out of equilibrium. The introduction of a catalyst, which introduces a second catalytic cycle into the metabolic network, was used to close the first cycle. This shows how coupled catalytic cycles can create a metabolic network that allows the creation and perseverance of fuel-driven, out-of-equilibrium self-replicating vesicles. One of the great challenges in chemistry is to mimic the complex living structures found in nature by designing artificial out-of-equilibrium systems. [1] These structures involve functional, dynamic states which require a continuous supply of energy to be sustained. [2] This energy can be supplied as a chemical fuel, or another energy source such as light. [3] Systems that use an energy supply to maintain behaviour that would otherwise not persist, are known as dissipative systems. [4] Upon depletion or removal of the energy source, the rates of destruction and formation become unbalanced and the system moves to thermodynamic equilibrium. By maintaining complex systems in an out-ofequilibrium state different behaviour can be observed compared to systems in equilibrium. This includes novel selfassembly processes, [5] material properties [6] and functionality. [7] Out-of-equilibrium systems are frequently encountered in phase-separated systems, where functionalities such as product selection [8] or self-regulation [9] can be observed. Especially interesting is the creation of synthetic supramolecular vesicles, since these resemble cellular structures found in nature. [10] Both functional transient vesicles, [11] and thermo-[*] Dr. A

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A synthetic metabolic network for physicochemical homeostasis

Cited by 58 publications

References 53 publications

The Rise of the Nested Multicompartment Model in Synthetic Cell Research

The Rise of the Nested Multicompartment Model in Synthetic Cell Research

Ciencia ficción de hoy, ciencia de mañana: construir una vida

Coupled Metabolic Cycles Allow Out‐of‐Equilibrium Autopoietic Vesicle Replication

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