A synthetic light-inducible photorespiratory bypass enhances photosynthesis to improve rice growth and grain yield

Xu, Huawei; Zhang, Yongfeng; Yang, Xiaoyi; Lv, Shufang; Hou, Dan; Mo, Chimei; Wassie, Misganaw; Yu, Bo; Tao, Hongbin

doi:10.1016/j.xplc.2023.100641

Cited by 4 publications

(7 citation statements)

References 47 publications

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“…Such a task is realized by synthetic promoter sets of exactly adjusted activities [195,[197][198][199][200][201][202][203]. The precise regulation of co-expressed genes in complex metabolic traits by synthetic promoters of different strengths protects against imbalance in metabolite concentration, feedback regulation of induction and energy losses [27,178,194]. However, the repeated use of the same or related DNA native promoter sequences, combined with conventional, multi-round breeding techniques, results in homology-based gene silencing, plasmid instability, complex segregation patterns, and the random integration of multiple transgenes expressed at varied and poorly controlled levels [156,157,165,166].…”

Section: Discussionmentioning

confidence: 99%

“…Cis-active elements are introduced according to their positions, copy number, distance, and orientation to promote optimum spacing among the cis-motifs and corresponding trans-factors, which could mutually interact and build functional dior oligomer complexes [182][183][184][185][186]188]. The prepared synthetic promoters demonstrate different tissue specificities, as well as strengths, and inducibility properties to precisely control the co-expression of numerous genes in complex biosynthesis traits [189][190][191][192][193][194]. In addition to the exchange of domains or cis-elements, novel synthetic promoter variants can also be prepared by the introduction of genetic changes into existing cis-active elements and core promoters [89,214].…”

Section: Discussionmentioning

confidence: 99%

“…The obtained promoters possess different tissue-specific, strength, and inducibility properties, enabling the co-expression of numerous genes at different levels, as is required by the complex biosynthesis traits or regulatory circuits responding to various environmental conditions, i.e., biotic, abiotic, tissue-specific, light stress, or hormonal stimulation [184,185,[189][190][191][192][193][194]. To avoid unwanted recombination or homology-dependent gene silencing, the expression of each gene is regulated by a different promoter [189,195].…”

Section: Objectives and General Methodology Of Synthetic Promoter Cre...mentioning

confidence: 99%

“…CaMV35S and the maize UBIQUITIN promoter (pUbi), were exercised to avoid gene-silencing effects while driving Cucurbita maxima malate synthase (CmMS) and O. sativa ascorbate peroxidase 7 (OsAPX7), respectively. However, the third gene in the GMA bypass, known as O. sativa glycolate oxidase 1 (OsGLO1), should be controlled by a light-inducible Rubisco small subunit promoter to dynamically adapt the gene expression rate to changes in light conditions and significantly improve photosynthetic efficiency [194].…”

Section: Cis-element Juggling and Domain Swappingmentioning

confidence: 99%

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Plant Synthetic Promoters

Szymczyk,

Majewska

2024

Applied Sciences

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This article examines the structure and functions of the plant synthetic promoters frequently used to precisely regulate complex regulatory routes. It details the composition of native promoters and their interacting proteins to provide a better understanding of the tasks associated with synthetic promoter development. The production of synthetic promoters is performed by relatively small libraries produced generally by basic molecular or genetic engineering methods such as cis-element shuffling or domain swapping. The article also describes the preparation of large-scale libraries supported by synthetic DNA fragments, directed evolution, and machine or deep-learning methodologies. The broader application of novel, synthetic promoters reduces the prevalence of homology-based gene silencing or improves the stability of transgenes. A particularly interesting group of synthetic promoters are bidirectional forms, which can enable the expression of up to eight genes by one regulatory element. The introduction and controlled expression of several genes after one transgenic event strongly decreases the frequency of such problems as complex segregation patterns and the random integration of multiple transgenes. These complications are commonly observed during the transgenic crop development enabled by traditional, multistep transformation using genetic constructs containing a single gene. As previously tested DNA promoter fragments demonstrate low complexity and homology, their abundance can be increased by using orthogonal expression systems composed of synthetic promoters and trans-factors that do not occur in nature or arise from different species. Their structure, functions, and applications are rendered in the article. Among them are presented orthogonal systems based on transcription activator-like effectors (dTALEs), synthetic dTALE activated promoters (STAPs) and dCas9-dependent artificial trans-factors (ATFs). Synthetic plant promoters are valuable tools for providing precise spatiotemporal regulation and introducing logic gates into the complex genetic traits that are important for basic research studies and their application in crop plant development. Precisely regulated metabolic routes are less prone to undesirable feedback regulation and energy waste, thus improving the efficiency of transgenic crops.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Objectives and General Methodology Of Synthetic Promoter Cre...mentioning

confidence: 99%

Section: Cis-element Juggling and Domain Swappingmentioning

confidence: 99%

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Plant Synthetic Promoters

Szymczyk,

Majewska

2024

Applied Sciences

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“…Obtained promoters indicate different tissue-specific, strength, and inducibility properties to enable the co-expression of numerous genes at different levels, as is required by the complex biosynthesis traits or regulatory circuits responding to different environmental conditions, i.e., biotic, abiotic, tissue-specific, light-stress, or hormonal-stimulation conditions [184,185,[189][190][191][192][193][194]. To avoid unwanted recombination or homology-dependent gene silencing, the expression of each gene is regulated by a different promoter [189,195].…”

Section: Objectives and General Methodology Of Synthetic Promoter Cre...mentioning

confidence: 99%

Plant Synthetic Promoters

Szymczyk,

Majewska

2024

Preprint

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The article features the structure and functions of plant synthetic promoters frequently exercised to precisely regulate complex regulatory routes. The composition of plant native promoters together with interacting proteins is presented to provide a better understanding of tasks associated with synthetic promoter development. The production of synthetic promoters is conferred on relatively small libraries produced generally by basic molecular or genetic engineering methods such as cis-element shuffling or domain swapping. Moreover, the preparation of large-scale libraries supported by synthetic DNA fragments, directed evolution, and machine or deep learning methodologies is presented. A particularly interesting group of synthetic promoters are bidirectional forms that enable the putative expression of up to 6–8 genes by one regulatory element. The introduction and controlled expression of several genes after one transgenic event strongly decreases the frequency of such problems as complex segregation patterns and random integration of multiple transgenes. These complications are commonly observed during transgenic crop development through traditional, multistep transformation by genetic constructs containing a single gene. Another path to solving problems associated with the low complexity and homology of already tested DNA fragments is through orthogonal expression systems composed of synthetic promoters and trans-factors that do not occur in nature or arise from different species. Their structure, functions, and applications are rendered in the article.

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