A synthetic enamel for rapid tooth repair

Yamagishi, Kazue; Onuma, Kazuo; Suzuki, Takashi; Okada, Fumio; Tagami, Junji; Otsuki, Masayuki; Senawangse, Pisol

doi:10.1038/433819a

Cited by 227 publications

(194 citation statements)

References 8 publications

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“…25 The reaction solutions were purged with nitrogen gas saturated with water vapor at 37 ± 0.1 °C. The relative supersaturation σ and supersaturation ratio S are given by eq 1: (1) in which υ (=18) is the number of ions in a formula unit of the HAP phase and IAP and K sp are the ionic activity and thermodynamic solubility product (5.52 × 10 −118 mol 18 ) and potassium dihydrogen phosphate (0.020 mol L −1 ) with sodium chloride to maintain the ionic strength I = 0.150 mol L −1 . Amel proteins were added to supersaturated reaction solutions to achieve concentrations ranging from 0.5 to 5.0 μg mL −1 .…”

Section: Hap Crystallization By Constant Compositionmentioning

confidence: 99%

“…10-14 Others have examined applications of external fields 15 and the use of various hydrothermal conditions, including extreme temperature, pressure, and pH. [16][17][18] However, these in vitro studies have not focused on the initial contact between mineral nuclei and organic additives that drive the earliest nucleation events responsible for subsequent directed assembly, yielding the complex morphologies and mineral phases found in enamel.…”

Section: Introductionmentioning

confidence: 99%

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Mimicking the Self-Organized Microstructure of Tooth Enamel

et al. 2008

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Under near-physiological pH, temperature, and ionic strength, amelogenin (Amel) accelerates hydroxyapatite (HAP) nucleation kinetics, decreasing the induction time in a concentrationdependent manner. Hierarchically organized apatite microstructures are achieved by self-assembly involving nucleated nanocrystallites and Amel oligomers and nanospheres at low supersaturations and protein concentrations in a slow and well-controlled constant composition (CC) system. The CC method allows the capture of an intermediate structure, the nanorod, following the formation of the critical nuclei at the earliest nucleation stages of calcium phosphate crystallization. The nanorod building blocks form spontaneously by synergistic interactions between flexible Amel protein assemblies and rigid calcium phosphate nanocrystallites. These intermediate structures further assemble by a self-epitaxial growth mechanism to form the final hierarchically organized microstructures that are compositionally and morphologically similar to natural enamel. This in vitro observation provides direct evidence that Amel promotes apatite crystallization and organization. We interpret our observations to propose that in vivo Amel may maximally exert an influence on the structural control of developing enamel crystals at the earliest nucleation stages.

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Section: Hap Crystallization By Constant Compositionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Mimicking the Self-Organized Microstructure of Tooth Enamel

et al. 2008

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“…Recently Yamagishi et al has reported that it is possible to use the fluoridated-apatite to repair early caries lesions directly. 7 We believe that the shape and chemical composition of well defined synthetic fluorapatite nanorods / nanowires have the potential to be layered over carious lesions.…”

Section: Introductionmentioning

confidence: 99%

Synthesis of Fluorapatite Nanorods and Nanowires by Direct Precipitation from Solution

Chen

Sun

Tang

et al. 2006

Crystal Growth & Design

108

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In natural tooth enamel fluoride is always present in the carbonated hydroxyapatite mineral and plays a key role in the prevention of tooth decay. In this study we aimed at mimicking this natural anticaries ability of the tooth by developing new, effective anticaries materials using fluorapatite nanorods or nanowires. We therefore investigated the conditions necessary to synthesize fluorapatite nanorods of different size, shape and composition for future use either directly or indirectly, that is by incorporation into dental materials, in the treatment and prevention of caries. By controlling the chemical conditions, nanorods of desirable chemical composition and dimension were produced. The mechanism of how these structures were formed is also proposed.

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“…This might occur because HAp initially precipitated at high temperature at the initial pH of 6.0 37) and then the HAp crystal grew epitaxially, or because fibrous OCP nanocrystals were initially formed and then hydrolyzed into HAp crystals 38) during the high temperature treatment at 80°C. The resultant fiber HAp had a lower Ca/P ratio as compared with that of the rodshaped HAp prepared under alkaline conditions, which indicates that calcium ions were exchanged with protons under acidic conditions; hence, HPO 3 2− was observed in the FT-IR spectrum.…”

Section: Discussionmentioning

confidence: 99%

“…Various attempts have been undertaken to harden the enamel surfaces or to remineralize tooth enamel by using fluoride 1) , metastable calcium phosphate (CaP) solution 2) , or acidic phosphate fluoride solution 3) treatments. Recently, the direct application of nanoparticulate hydroxyapatite (HAp) -one of the most widely used types of CaP materials 4)-for repairing (remineralizing or hardening) damaged enamel has attracted attention [5][6][7] .…”

Section: Introductionmentioning

confidence: 99%

Adsorption and desorption behaviors of cetylpyridinium chloride on hydroxyapatite nanoparticles with different morphologies

et al. 2016

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Application of hydroxyapatite (HAp) nanoparticles to repair damaged enamel has attracted recent attention. In this study, HAp nanoparticles with various morphologies (spherical, short-rod, long-rod and fiber morphologies) were synthesized via chemical precipitation methods without the addition of template molecules, and the adsorption/desorption behaviors of a cationic antibacterial agent, cetylpyridinium chloride (CPC), on the HAp nanoparticles were evaluated. The adsorption of CPC on each HAp nanoparticle showed Langmuir-type adsorption, and the short-rod/long-rod HAp nanoparticles showed thermodynamically more stable adsorption of CPC than that with the spherical/fiber HAp nanoparticles. The desorption rate of CPC from the short-rod/long-rod HAp nanoparticles was slower than that of the spherical/fiber HAp nanoparticles. The HAp nanoparticles with different CPC release profiles presented here have potential applications as nanoparticulate enamel repair agents with antibacterial properties.

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A synthetic enamel for rapid tooth repair

Cited by 227 publications

References 8 publications

Mimicking the Self-Organized Microstructure of Tooth Enamel

Mimicking the Self-Organized Microstructure of Tooth Enamel

Synthesis of Fluorapatite Nanorods and Nanowires by Direct Precipitation from Solution

Adsorption and desorption behaviors of cetylpyridinium chloride on hydroxyapatite nanoparticles with different morphologies

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