A synthetic construct for genetic engineering of the emerging pathogenic yeast Candida auris

Transcriptome Assembly and Profiling of Candida auris Reveals Novel Insights into Biofilm-Mediated Resistance

“…Given that we can now genetically manipulate this pathogenic yeast ( 55 , 56 ), future work analyzing the functional roles and processes of specific genes and proteins will further enhance our understanding of biofilm-associated pathogenicity and resistance. Unraveling the key factors that regulate the transcriptional network that exists for C. auris , similar to those studies in C. albicans and Candida parapsilosis ( 26 , 57 ), may provide translational insights into novel avenues for therapeutic targets for biofilm-associated infections.…”

Tuberculose Pulmonar: Perfil Epidemiológico Do Sertão Pernambucano, Brasil

“…Avanços no sequenciamento genômico foram relatados nos estudos avaliados (DEFOSSE et al, 2017;SHARMA et al, 2015;SHARMA et al, 2016;MOHD et al, 2018). Um estudo realizado com cepas de quatro hospitais indianos, a partir do seu sequenciamento genômico, levantou a evidência que pudesse haver transmissão clonal (SHARMA et al, 2016), enquanto uma investigação filogenética aferiu que a origem de cepas isoladas, no denominado maior surto de infecções por C. auris, no Reino Unido tinha origem asiática (RHODES et al, 2018).…”

What do we know about the biology of the emerging fungal pathogen of humans Candida auris?

“…C. auris can be transformed by electroporation or by making it chemically competent for the uptake of genome alteration cassettes (Bravo Ruiz et al, 2020;Defosse et al, 2018;Grahl et al, 2017;Mayr et al, 2020). Currently, there are three dominant drug resistance cassettes available to introduce and select for the desired constructs, these cassettes confer resistance to the antibiotics nourseothricin, hygromycin B, and mycophenolic acid (Defosse et al, 2018).…”