A synopsis of <i>Ipomoea (Convolvulaceae)</i> in the Americas

Austin, Daniel F.; Huamán, Z.

doi:10.2307/1222581

Cited by 229 publications

(191 citation statements)

References 24 publications

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“…The fl oral structure of the genus Ipomoea is almost the same, a gamopetalous fl ower, resembling that of the morning glory. This genus Ipomoea is classifi ed into several sections based on reproductive and morphological traits (Austin and Huáman 1996 ). For instance, the section Pharbitis includes both the self-incompatible Ipomoea serifera and the self-compatible species I. purpurea ; both horticultural varieties are known as morning glory.…”

Section: Origin Domestication and Compatibility Of Ipomoea Plantsmentioning

confidence: 99%

Self-Incompatibility System of Ipomoea trifida, a Wild-Type Sweet Potato

Tsuchiya

2014

Sexual Reproduction in Animals and Plants

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Diploid Ipomoea trifi da (Convolvulaceae) is a close relative of the cultivated hexaploid Ipomoea batatas , the cultivated sweet potato. These plants have sporophytic self-incompatibility that is regulated by a single multiallelic locus, designated as the S -locus. Genetic analyses of I. trifi da plants collected from Central America identifi ed about 50 different S -haplotypes with a linear dominance hierarchy having some codominance relationships. A linkage map of DNA markers around the S -locus indicated that the S -locus is delimited to 0.23 cM. Within the S -locus genomic region, a hypervariable genomic region of 35-95 kbp was identifi ed, and we designated this region SDR ( S -locus-specifi c divergent region). Of the several genes located within the SDR, one anther-specifi c gene, AB2 , and three stigma-specifi c genes, SE1 , SE2 , and SEA , are candidate S -genes that may encode male and female S -determinants of self-incompatibility.Keywords Ipomoea trifi da • Self-incompatibility • Sweet potato IntroductionSelf-incompatibility (SI) is a genetic mechanism to prevent self-fertilization (and thus encourage outcrossing) in angiosperms. In self-incompatible plants, when a pollen grain is recognized as the same type as self, some stage of pollen germination, pollen tube elongation, ovule fertilization, or embryo development is halted, and no seeds are produced. SI is classifi ed into several groups: homomorphic SI, heteromorphic SI, cryptic SI (CSI), and late-acting SI. Heteromorphic SI is classifi ed into two groups: distyly is determined by a single locus, which has two alleles, and tristyly is determined by two loci, each with two alleles. Heteromorphic SI is sporophytic, in that both alleles in the male plant determine the SI response in the pollen. CSI exists in a limited number of taxa (for example, there is evidence for CSI in Silene vulgaris in the Caryophyllaceae; Glaettli 2004 ). In this mechanism, the simultaneous presence of cross-and self-pollen on the same stigma results in higher seed set from cross-pollen (Bateman 1956 ). Late-acting SI is also termed ovarian SI. In this mechanism, self-pollen germinates and reaches the ovules, but no fruit is set (Seavey and Bawa 1986 ;Sage et al. 1994 ).Homomorphic SI is classifi ed into sporophytic SI (SSI) and gametophytic SI (GSI) . The SSI system is found in species of several plant families, such as the Brassicaceae, Asteraceae, Malvaceae, Betulaceae, Sterculiaceae, Polemoniaceae, and Convolvulaceae (de Nettancourt 2001 ; Allen and Hiscock 2008 ). In the plants in these families, SI is genetically regulated by a single multi-allelic locus, the S -locus . Within the S -locus, a pair of genes (named S -genes ), one encoding the male-determinant molecules and the other the female-determinant molecules, is localized. These genes are essential for the SI reaction, because these gene products contribute to self/non-self recognition. The sets of S -genes are tightly linked at the S -locus, and the S -locus (also called S -haplotype : Nasrallah and Na...

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Section: Origin Domestication and Compatibility Of Ipomoea Plantsmentioning

confidence: 99%

Self-Incompatibility System of Ipomoea trifida, a Wild-Type Sweet Potato

Tsuchiya

2014

Sexual Reproduction in Animals and Plants

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“…Quamoclit section Mina) occurs in Paraguay, Bolivia and from north to central Argentina; in contrast, I. purpurea (L.) Roth (Ipomoea subg. Ipomoea or Quamoclit, section Pharbitis) is a geographically widespread species, originally from the Americas but currently with a cosmopolitan distribution (Austin and Huaman, 1996). The actual geographic distribution of I. purpurea includes the distribution of I. rubriflora.…”

Section: Study Species and Sitesmentioning

confidence: 99%

Plant recruitment and range size: Transition probabilities from ovule to adult in two Ipomoea

2013

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This article appeared in a journal published by Elsevier. The attached copy is furnished to the author for internal non-commercial research and education use, including for instruction at the authors institution and sharing with colleagues.Other uses, including reproduction and distribution, or selling or licensing copies, or posting to personal, institutional or third party websites are prohibited. b s t r a c tIn plants, narrow geographic distributions are generally associated with low colonization and persistence abilities, therefore narrowly distributed plants are expected to have lower plant recruitment success than widespread species. Determining the association between recruitment success and range size requires the comparison of the success in multiple life-history stages among narrowly distributed and widespread congeners sharing the same habitat, an integrated approach rarely considered. We compared transition probabilities from ovule to reproductive adult between the narrowly distributed annual vine Ipomoea rubriflora O'Donnell (Convolvulaceae) and the widespread Ipomoea purpurea (L.) Roth at sites in Chaco woodland where they co-occur. I. rubriflora had marginally lower ovule fertilization success, a lower probability of seed maturation and lower seedling establishment than I. purpurea. The lowest transition probability for both species was seedling emergence. Seedling establishment in I. rubriflora was similar to seedling emergence. Plant recruitment success in I. rubriflora was an order of magnitude lower than that of I. purpurea. Indeed, I. rubriflora had lower total seed production per plant and smaller seed mass. Our results suggest that understanding processes determining regeneration (i.e., seedling emergence and establishment) may be of high importance for understanding narrow distributions in annual plants. The results also suggest that the narrowly distributed species is not only at a "numerical disadvantage" for colonizing new sitesdi.e., lower total seed production per plantdbut it also has a lower per-seed probability of establishing and thus to persist. However, as lower establishment success in this species seemed to be associated with the lower ability to survive adverse conditions of seedlings from smallerseeded species, a narrower regeneration niche might also explain its narrow distribution.

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“…Electrophoresis was conducted in a 1X TBE buffer [100 mL 10X TBE (0.89M Tris base, 0.89M Boric acid, 20 mM EDTA pH 8.0) and 900 mL distilled water] at 60 V for 30 min and then at 120 V for 1.5 h. The gel was then stained in silver nitrate (Bassam et al, 1991), using 125 mL fixation buffer (10% absolute ethanol, 5% absolute acetic acid, 895 mL distilled water) and 0.2% silver nitrate for 5 min. The gel then was washed twice with 150 mL distilled water, and stained with 125 mL revelation buffer (30% sodium hydroxide in 1000 mL distilled water), adding 0.4% phormaldeid.…”

Section: Dna Quantificationmentioning

confidence: 99%

CTAB methods for DNA extraction of sweetpotato for microsatellite analysis

Borges

Rosa

Recchia

et al. 2009

Sci. agric. (Piracicaba, Braz.)

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Microsatellite markers have proved to be useful in genetic diversity assessments of sweetpotato (Ipomoea batatas) but practical DNA extraction methods to ensure good quality and quantity DNA for these studies are yet to be established. This study compares the efficiency of three modified methodologies for DNA extraction of six sweetpotato landraces using the CTAB extraction buffer in regard to quantity and purity of DNA quantification and microsatellite band patterns. All methodologies yielded satisfactory results, but the method based in leaf tissue macerated in liquid nitrogen was deemed more adequate because of its simplicity and lower cost. However, the method based in dry leaf tissue was considered more advantageous, first because elicits practicability in the plant acquisition and drying process, especially when the collection is performed in situ, and also because its simplicity makes possible the cold storage of the dry, ground samples for future DNA extractions. Key words: Ipomoea batatas, SSR, DNA isolation, landraces, protocol MÉTODOS CTAB DE EXTRAÇÃO DE DNA PARA A ANÁLISE DE MICROSSATÉLITES EM BATATA-DOCERESUMO: Os marcadores microssatélites são úteis para a análise da diversidade genética de variedades tradicionais de batata-doce (Ipomoea batatas). Para estes estudos, métodos práticos de extração de DNA precisam ser estabelecidos para assegurar uma boa qualidade e quantidade de DNA extraído. Assim, foi comparada a eficiência de três metodologias para extração de DNA usando o tampão de extração CTAB, todas com modificações. Para verificar a quantidade e pureza na quantificação de DNA, bem como o padrão de bandas de microssatélites para as três metodologias utilizaram-se seis etnovariedades de batata-doce. Os testes mostraram que as três metodologias apresentaram resultados satisfatórios. Uma das metodologias baseada em tecido foliar macerado em nitrogênio líquido mostrouse a mais adequada devido à simplicidade e menor custo. Entretanto, o método baseado em tecido foliar seco foi o mais vantajoso devido à praticidade na aquisição da planta e no processo de secagem, principalmente quando a coleção encontra-se em condições in situ, e pela possibilidade do armazenamento refrigerado das amostras secas e maceradas para futuras extrações de DNA.

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A synopsis of Ipomoea (Convolvulaceae) in the Americas

Cited by 229 publications

References 24 publications

Self-Incompatibility System of Ipomoea trifida, a Wild-Type Sweet Potato

Self-Incompatibility System of Ipomoea trifida, a Wild-Type Sweet Potato

Plant recruitment and range size: Transition probabilities from ovule to adult in two Ipomoea

CTAB methods for DNA extraction of sweetpotato for microsatellite analysis

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