A synergistic combination of Phyllanthus emblica and Alpinia galanga against H 2 O 2 -induced oxidative stress and lipid peroxidation in human ECV304 cells

Chansriniyom, Chaisak; Bunwatcharaphansakun, Phichaporn; Eaknai, Waleewan; Nalinratana, Nonthaneth; Ratanawong, Arkornnut; Khongkow, Mattaka; Luechapudiporn, Rataya

doi:10.1016/j.jff.2018.01.016

Cited by 16 publications

(9 citation statements)

References 47 publications

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“…It was found that the potent and active ingredient is quercetin, which at 10 μg/mL concentration reduced the H 2 O 2 -mediated lipid peroxidation. When combined with ellagic acid and hydroxycinnamic derivatives, quercetin may aid antioxidant-mediated cytoprotection [ 47 ].…”

Section: Biological Activitiesmentioning

confidence: 99%

Chemical Components and Biological Activities of the Genus Phyllanthus: A Review of the Recent Literature

Nisar

Ahmed

et al. 2018

Molecules

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Medicinal plants have served humans since prehistoric times to treat various ailments. Both developed and underdeveloped countries rely on traditional systems of medication using natural sources from plants. Phyllanthus is one of the largest genus in the family Phyllanthaceae, comprising over 700 well known species cosmopolitan in distribution mainly in the tropics and subtropics. Phyllanthus species are being in constant used in traditional medications to cure an array of human diseases (constipation, inhalation related, arthritis, loss of appetite, injuries, conjunctivitis, diarrhoea, running nose, common cold, malaria, blennorrhagia, colic, diabetes mellitus, dysentery, indigestion, fever, gout, gonorrheal diseases of males and females, skin itching, jaundice, hepatic disorders, leucorrhea, vaginitis, menstrual irregularities, obesity, stomach pains, and tumors), confectionaries, food industry, and in some pesticides. Phyllanthus species are rich in diversity of phytochemicals e.g., tannins, terpenes, alkaloids, glycosidic compounds, saponins, and flavones etc. More in depth studies are a direly needed to identify more compounds with specific cellular functions to treat various ailments.

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Section: Biological Activitiesmentioning

confidence: 99%

Chemical Components and Biological Activities of the Genus Phyllanthus: A Review of the Recent Literature

Nisar

Ahmed

et al. 2018

Molecules

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“…Briefly, the cells were treated with ethyl acetate extracts for 24 h and then incubated with 10 µM DCFH-DA in a serum-free medium for 30 mins and washed with phosphate buffer solution (PBS) before 100 µM H 2 O 2 induction for 30 mins. After removal the medium, 100 µl PBS was added and the fluorescence intensities were measured at an excitation and emission wavelengths of 485 and 530 nm, respectively [46].…”

Section: Antioxidant Activitiesmentioning

confidence: 99%

“…The 1,1-diphenyl 2-picrylhydrazyl (DPPH) radical scavenging activity of ethyl acetate extract was determined according to Chansriniyom et al . [46]. Nitric oxide generated from sodium nitroprusside interacts with oxygen to produce nitrite ions with a purple chromophore through Griess reaction [47].…”

Section: Antioxidant Activitiesmentioning

confidence: 99%

Streptomyces barringtoniae sp. nov., isolated from rhizosphere of plant with antioxidative potential

Wai

Luechapudiporn

Tedsree

et al. 2022

International Journal of Systematic and Evolutionary Microbiology

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A novel actinomycete strain, JA03T, belonging to the genus Streptomyces , was isolated from the rhizosphere of Barringtonia racemosa (L.) Spreng. It was characterized taxonomically using a polyphasic approach. It grew at 25–37 °C, at pH 5–10 and with 6 % (w/v) NaCl. It contained ll-diaminopimelic acid in the cell-wall peptidoglycan. Ribose and glucose were detected in its whole-cell hydrolysate. The predominant cellular fatty acids were iso-C16 : 0, anteiso-C15 : 0, C16 : 0, iso-C14 : 0 and iso-C15 : 0. Detected polar lipids were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannosides, unidentified phospholipids and unidentified amino lipids. Based on the results of 16S rRNA gene sequence analyses, strain JA03T showed highest similarity to Streptomyces filipinensis NBRC 12860T (98.76 %), Streptomyces fodineus TW1S1T (98.69 %) and Streptomyces shenzhennensis 172115T (98.68 %). Strain JA03T has a genome size of 9 092 851 bp with DNA G+C content of 71.28 mol%. The average nucleotide identity (ANI)-blast and ANI-MUMmer values of strain JA03T and related type strains were 79.6–89.2 and 86.7–92.5 %, respectively, and the digital DNA–DNA hybridization values were 27.3–46.4 %. Ethyl acetate extract of JA03T exhibited total phenolic content (33.4±0.6 µg mg−1 gallic acid equivalent), ferric reducing power value (70.8±1.8 µg mg−1 ascorbic acid equivalent) and 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity (IC50=67.0±21.1 µg ml−1). Intracellular reactive oxygen species and NO production in RAW264.7 macrophage cells induced by H2O2 and lipopolysaccharide were inhibited with IC50 of 67.40 and 16.95 µg ml−1, respectively. Based on the taxonomic results, it has been concluded that strain JA03T represents a novel species of the genus Streptomyces for which the name Streptomyces barringtoniae sp. nov., is proposed. The type strain is JA03T (=LMG 32415T=TISTR 2999T).

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“…In addition, it is traditionally applied for treating dyspepsia and abdominal colic pain, motion sickness, and gastralgia [ 15 , 16 ]. Up to now, there are numerous articles about pharmacological activities of A. galanga , for instance antiallergic [ 17 ], anticancer [ 18 , 19 , 20 ], anti-inflammatory [ 21 ], antimicrobial [ 21 , 22 , 23 , 24 ], antioxidant [ 24 , 25 ], anti-amnesiac [ 26 ], antiasthma [ 27 ], and also melanogenesis inhibitor [ 28 ]. However, there is no report about its positive activity as an antiparasitic agent [ 29 ].…”

Section: Introductionmentioning

confidence: 99%

Six New Phenylpropanoid Derivatives from Chemically Converted Extract of Alpinia galanga (L.) and Their Antiparasitic Activities

et al. 2021

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Chemical conversion of the extract of natural resources is a very attractive way to expand the chemical space to discover bioactive compounds. In order to search for new medicines to treat parasitic diseases that cause high morbidity and mortality in affected countries in the world, the ethyl acetate extract from the rhizome of Alpinia galanga (L.) has been chemically converted by epoxidation using dioxirane generated in situ. The biological activity of chemically converted extract (CCE) of A. galanga (L.) significantly increased the activity against Leishmania major up to 82.6 ± 6.2 % at 25 μg/mL (whereas 2.7 ± 0.8% for the original extract). By bioassay-guided fractionation, new phenylpropanoids (1–6) and four known compounds, hydroquinone (7), 4-hydroxy(4-hydroxyphenyl)methoxy)benzaldehyde (8), isocoumarin cis 4-hydroxymelein (9), and (2S,3S,6R,7R,9S,10S)-humulene triepoxide (10) were isolated from CCE. The structures of isolated compounds were determined by spectroscopic analyses of 1D and 2D NMR, IR, and MS spectra. The most active compound was hydroquinone (7) with IC50 = 0.37 ± 1.37 μg/mL as a substantial active principle of CCE. In addition, the new phenylpropanoid 2 (IC50 = 27.8 ± 0.34 μg/mL) also showed significant activity against L. major compared to the positive control miltefosine (IC50 = 7.47 ± 0.3 μg/mL). The activities of the isolated compounds were also evaluated against Plasmodium falciparum, Trypanosoma brucei gambisense and Trypanosoma brucei rhodeisense. Interestingly, compound 2 was selectively active against trypanosomes with potent activity. To the best of our knowledge, this is the first report on the bioactive “unnatural” natural products from the crude extract of A. galanga (L.) by chemical conversion and on its activities against causal pathogens of leishmaniasis, trypanosomiasis, and malaria.

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A synergistic combination of Phyllanthus emblica and Alpinia galanga against H 2 O 2 -induced oxidative stress and lipid peroxidation in human ECV304 cells

Cited by 16 publications

References 47 publications

Chemical Components and Biological Activities of the Genus Phyllanthus: A Review of the Recent Literature

Chemical Components and Biological Activities of the Genus Phyllanthus: A Review of the Recent Literature

Streptomyces barringtoniae sp. nov., isolated from rhizosphere of plant with antioxidative potential

Six New Phenylpropanoid Derivatives from Chemically Converted Extract of Alpinia galanga (L.) and Their Antiparasitic Activities

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