A synchrotron radiation microtomography system for the analysis of trabecular bone samples

Salomé, Murielle; Peyrin, Françoise; Cloetens, Peter; Odet, C.; Laval-Jeantet, A. M.; Baruchel, J.; Spanne, P.

doi:10.1118/1.598736

Cited by 253 publications

(190 citation statements)

References 36 publications

(33 reference statements)

Supporting

Mentioning

190

Contrasting

Order By: Relevance

“…The acquisition setup is based on 3D parallel tomography described in a detailed manner in (Salome et al, 1999). Briefly, the sample is placed on a rotating platform and partially stops the incident monochromatic beam.…”

Section: Image Acquisition: Synchrotron Radiation Computed Microtomogmentioning

confidence: 99%

“…Briefly, the sample is placed on a rotating platform and partially stops the incident monochromatic beam. The number of transmitted photons is measured with a twodimensional (2D) detector, which consists of a Gadox scintillator (an X-ray-to-visible light converter), light optics for magnification of the image, and a FReLoN CCD camera developed at the ESRF (Salome et al, 1999). The camera has 14-bit dynamic range and a 2,048 Â 2,048 pixel chip.…”

Section: Image Acquisition: Synchrotron Radiation Computed Microtomogmentioning

confidence: 99%

See 1 more Smart Citation

Kinetics of in vivo bone deposition by bone marrow stromal cells within a resorbable porous calcium phosphate scaffold: An X‐ray computed microtomography study

Papadimitropoulos

Mastrogiacomo

Peyrin

et al. 2007

Biotech & Bioengineering

View full text Add to dashboard Cite

Resorbable ceramic scaffolds based on Silicon stabilized tricalcium phosphate (Si-TCP) were seeded with bone marrow stromal cells (BMSC) and ectopically implanted for 2, 4, and 6 months in immunodeficient mice. Qualitative and quantitative evaluation of the scaffold material was performed by X-ray synchrotron radiation computed microtomography (microCT) with a spatial resolution lower than 5 mm. Unique to these experiments was that microCT data were first collected on the scaffolds before implantation and then on the same scaffolds after they were seeded with BMSC, implanted in the mice and rescued after different times. Volume fraction, mean thickness and thickness distribution were evaluated for both new bone and scaffold phases as a function of the implantation time. New bone thickness increased from week 8 to week 16. Data for the implanted scaffolds were compared with those derived from the analysis of the same scaffolds prior to implantation and with data derived from 100% hydroxyapatite (HA) scaffold treated and analyzed in the same way. At variance with findings with the 100% HA scaffolds a significant variation in the density of the different Si-TCP scaffold regions in the pre-and post-implantation samples was observed. In particular a post-implantation decrease in the density of the scaffolds, together with major changes in the scaffold phase composition, was noticeable in areas adjacent to newly formed bone. Histology confirmed a better integration between new bone and scaffold in the Si-TCP composites in comparison to 100% HA composites where new bone and scaffold phases remained well distinct.

show abstract

Section: Image Acquisition: Synchrotron Radiation Computed Microtomogmentioning

confidence: 99%

Section: Image Acquisition: Synchrotron Radiation Computed Microtomogmentioning

confidence: 99%

Kinetics of in vivo bone deposition by bone marrow stromal cells within a resorbable porous calcium phosphate scaffold: An X‐ray computed microtomography study

Papadimitropoulos

Mastrogiacomo

Peyrin

et al. 2007

Biotech & Bioengineering

View full text Add to dashboard Cite

show abstract

“…SR ACT provides 3D images at very high spatial resolution with a high signal to noise ratio within a limited acquisition time [38]. Image acquisition was performed using a monochromatic X-ray beam fixed to 25 KeV.…”

Section: Materials and Protocolmentioning

confidence: 99%

“…Even if 3D images may now be provided from different techniques and modalities, it is worthwhile noting that spatial resolution strongly affects the accuracy of architectural parameters [35,36]. 3D images of bone samples with a spatial resolution higher than 10 Am and high signal to noise ratio may easily be achieved using synchrotron radiation (SR) ACT [37,38].…”

Section: Introductionmentioning

confidence: 99%

Relationship between compressive properties of human os calcis cancellous bone and microarchitecture assessed from 2D and 3D synchrotron microtomography

Follet

Bruyère-Garnier

Peyrin

et al. 2005

Bone

View full text Add to dashboard Cite

The aim of this study was to determine the contribution of 2D and 3D microarchitectural characteristics in the assessment of the mechanical strength of os calcis cancellous bone. A sample of cancellous bone was removed in a medio-lateral direction from the posterior body of calcaneus, taken at autopsy in 17 subjects aged 61-91 years. The sample was first used for the assessment of morphological parameters from 2D morphometry and 3D synchrotron microtomography (ACT) (spatial resolution = 10 Am). The 2D morphometry was obtained from three slices extracted from the 3D ACT images. Very good concordance was shown between 3D ACT slices and the corresponding physical histologic slices. In 2D, the standard histomorphometric parameters, fractal dimension, mean intercept length, and connectivity were computed. In 3D, histomorphometric parameters were computed using both the 3D mean intercept length method and model-independent techniques. The 3D fractal dimension and the 3D connectivity, assessed by Euler density, were also evaluated. The cubic samples were subjected to elastic compressive tests in three orthogonal directions (X, Y, Z) close to the main natural trabecular network directions. A test was performed until collapse of trabecular network in the main direction (Z). The mechanical properties were significantly correlated to most morphological parameters resulting from 2D and 3D analysis. In 2D, the correlation between the mechanical strength and bone volume/tissue volume was not significantly improved by adding structural parameters or connectivity parameter (nodes number/tissue volume). In 3D, one architectural parameter (the trabecular thickness, Tb.Th) permitted to improve the estimation of the compressive strength from the bone volume/tissue volume alone. However, this improvement was minor since the correlation with the BV/TV alone was high (r = 0.96). In conclusion, which is in agreement with the statistic's rules, we found, in this study, that the determination of the os calcis bone compressive strength using the 3D bone volume fraction cannot be improved by adding 3D architectural parameters.

show abstract

“…Each image is an axial cross-section composed by 1,024×1,024 voxels, 8 bit grey scale, with 20 × 20 µm 2 in-plane resolution and a slice thickness of 40 µm. Further details about the scanning and reconstruction procedure can be found elsewhere [10,27] .…”

Section: µCt Experimentsmentioning

confidence: 99%

Software tools for the quantitative evaluation of dental treatment effects from µCT scans

Sinibaldi

Conti

Pecci

et al. 2013

JBGC

View full text Add to dashboard Cite

Background: The 3D images of dental specimens were obtained by means of micro-Computed Tomography (µCT) before and after therapeutic intervention. A suite of software tools has been developed to assess the efficacy of dental treatment as revealed by µCT scans. Endodontic root canal and restorative therapy were selected as model procedures to test and optimize the developed tools. Non destructive µCT imaging allows repeated scans of the same tooth and might provide quantitative information about specimen modifications over time. Preliminary literature has suggested that anatomical characterization and detailed evaluation of dental therapies would considerably benefit from the coupling between detailed 3D imaging and custom software. However, the main drawbacks reside in time consuming pipeline for scanner-time of the specimens and absence of efficient software for accurate data analysis and direct comparison of a set of 3D dental structures. Methods:The software we present here implements co-registration procedures together with an algorithm for the 3D quantitative characterization of teeth hollow spaces and interfaces. The software is targeted to the individualized analysis of the single specimen in an effort to help dentists to quantify the outcome of the surgical intervention performed on teeth. The approach aims to automatically highlight the differences between pre-and post-treatment structures for further anatomical and statistical analysis. Of note, the two different treatments analyzed exploit two different perspectives. As a matter of facts, endodontic therapy aims at the modification of the root canal structure (axe, volume and surface), whereas the restorative treatment focus is on the whole tooth structure. Results:The developed co-registration algorithm allows finding correct superposition in both data sets, despite the preand post-structure can be markedly different, especially after the application of dental adhesive composite in the restorative treatment. Tools for characterization of canal volume and surface are robust with respect to the presence of branches and allow the complete characterization of the endodontic treatments. Modification of the tooth structure after restorative treatments is highlighted after the co-registration procedure in automatic way and allows the user to quantify the volume of voids due to the shrinkage of the material at the interface and eventually the presence of internal cracks or other morphological modification of the tooth structure.www.sciedu.ca/jbgc Journal of Biomedical Graphics and Computing, 2013, Vol. 3, No. 4 ISSN 1925-4008 E-ISSN 1925 86 Conclusions: We demonstrate the effectiveness of customization of 3D data analysis tools to obtain specific characterization and quantitative information about different dental therapies.

show abstract

A synchrotron radiation microtomography system for the analysis of trabecular bone samples

Cited by 253 publications

References 36 publications

Kinetics of in vivo bone deposition by bone marrow stromal cells within a resorbable porous calcium phosphate scaffold: An X‐ray computed microtomography study

Kinetics of in vivo bone deposition by bone marrow stromal cells within a resorbable porous calcium phosphate scaffold: An X‐ray computed microtomography study

Relationship between compressive properties of human os calcis cancellous bone and microarchitecture assessed from 2D and 3D synchrotron microtomography

Software tools for the quantitative evaluation of dental treatment effects from µCT scans

Contact Info

Product

Resources

About