A synbiotic marine oligosaccharide microcapsules for enhancing Bifidobacterium longum survivability and regulatory on intestinal probiotics

Abstract

A novel synbiotic multiparticulate microparticle containing alginate oligosaccharide (AOS), chitosan oligosaccharide (COS) and Bifidobacterium longum CICC 6259 was produced in the current study to expand the synbiotic industrial applications. The influences of these treatments on encapsulation yield, size, morphology, protective effect and stability of microcapsules and on mice gut microbiome were studied in vitro and in vivo. In vitro experiments detected no significant difference (P > 0.05) in encapsulation yield with different types of microcapsules. However, the microcapsule diameter of marine oligosaccharide was approximately 60 lm greater, and these microcapsules increased the number of surviving cells by more than 3 log cfu/g after treatment with simulated gastrointestinal juices, compared to basic alginate microcapsules. In vivo, these microcapsules significantly increased the content of Bifidobacterium and Lactobacillus and reduced the content of Enterococcus and Escherichia in mice gut microbiome. Marine oligosaccharide probiotic microcapsules are promising as a novel functional food ingredient.

The modulatory effect of encapsulated bioactives and probiotics on gut microbiota: improving health status through functional food

“…Another example of the modulatory effect of microencapsulated probiotics is the study carried out by Chang et al , 66 where Bifidobacterium longum was co-encapsulated with a marine oligosaccharide and administered to a group of mice after proving in vitro that the probiotic survives in an adequate concentration in gastrointestinal conditions. When administered to mice, these microcapsules significantly increased the content of Bifidobacterium and Lactobacillus and reduced the content of Enterococcus and Escherichia in the gut microbiome of the mice.…”

“…43,45 Also, microparticles encapsulating LGG by freeze drying allowed the probiotic to survive in an adequate concentration to colonize the colon; this was proved by the results obtained for the microbial community structure, which showed that LGG outcompeted other species of lactobacilli. 69 Another example of the modulatory effect of microencapsulated probiotics is the study carried out by Chang et al, 66 where Bifidobacterium longum was co-encapsulated with a marine oligosaccharide and administered to a group of mice after proving in vitro that the probiotic survives in an adequate concentration in gastrointestinal conditions. When administered to mice, these microcapsules significantly increased the content of Bifidobacterium and Lactobacillus and reduced the content of Enterococcus and Escherichia in the gut microbiome of the mice.…”

Biopolymer‐Based Multilayer Microparticles for Probiotic Delivery to Colon

“…Charged interactions ensure a secure and even coating, even when common materials are used such as alginate and chitosan. [66,[85][86][87] An example of this was the emulsification of free cells (B. longum) in an alginate hydrogel that were dip-coated in a chitosan solution. [70] This 36 μm coating slightly decreased the encapsulation yield, but dramatically increased survivability in simulated gastric fluid (SGF) (pH 2.5) and simulated intestinal fluid (SIF) with bile salts (pH 6.8).…”

Review on potential non‐dairy synbiotic beverages: a preliminary approach using legumes

“…However, regulating the processing of synbiotic products from the beginning of production to the end of the shelf life is needed for preserving consumer acceptance. For this purpose, several techniques can be used which primarily include fortifying the product with prebiotics and adding probiotic microcapsules (Dias et al, 2018;Chan et al, 2020).…”