A synbiotic improves the immunity of white shrimp, Litopenaeus vannamei: Metabolomic analysis reveal compelling evidence

Huynh, Truong‐Giang; Cheng, An; Chi, Chia‐Chun; Chiu, Kuo-Hsun; Liu, Chun-Hung

doi:10.1016/j.fsi.2018.05.031

Cited by 78 publications

(60 citation statements)

References 48 publications

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“…Recent advances in technologies and bioinformatic analyses have allowed the development of a broader understanding of the complex microbial communities associated with various habitats including the aquatic animal gut microbiota (Ghanbari, Kneifel, & Domig, ). As mentioned above, beneficial effects of a SYN on the growth performance (Huynh, Chi, et al, ) and health status (Huynh, Cheng, et al, ) of L. vannamei were found in our previous studies, but determining whether the positive effects involved changes in the shrimp's intestinal microbiota required further study.…”

Section: Introductionmentioning

confidence: 67%

“…Because adding the SYN, Lac. plantarum 7–40 at 10 8 cfu/kg diet and 0.4% GOS, to the diet resulted in the shrimp having better efficiency in improved growth and health conditions (Huynh, Cheng, et al, ; Huynh, Chi, et al, ), a SYN‐containing diet and control diet without SYN were used in this study. The basal diet was formulated to be sufficient to support the optimal growth of L. vannamei (Table ).…”

Section: Methodsmentioning

confidence: 99%

“…As the cost of feed ingredients and serious diseases increased, developing supplements or feed ingredient replacements to reduce feed costs and disease risk has become an international concern. Supplements, such as probiotics (Liu, Chiu, Ho, & Wang, ; Wang, ), immunostimulants (Hossain, Koshio, Manabu, Yokoyama, & Sony, ), or synbiotic (SYN) that can increase the probiotic efficiency are widely used in aquaculture to increase the growth and health status of cultured shrimp and fish (Hoseinifar, Mirvaghefi, Amoozegar, Merrifield, & Ringo, ; Huynh, Cheng, Chi, Chiu, & Liu, ; Huynh, Chi, et al, ; Makivuokko, Forssten, Saarinen, Ouwehand, & Rautonen, ). Among those products, SYNs are considered to be more effective in improving the growth performance and health status of aquatic animals (Huynh, Cheng, et al, ; Huynh, Chi, et al, ; Huynh et al, ).…”

Section: Introductionmentioning

confidence: 99%

“…Li, Tan, and Mai () reported that a SYN comprised of isomaltooligosaccharide (IMO) and Bacillus megeterium significantly increased the immune responses and disease resistance, but no information on growth was revealed for L. vannamei . In our previous studies, a SYN containing Lactobacillus plantarum 7–40 (10 8 colony‐forming units (cfu)/kg diet) and 0.4% GOS was formulated to improve shrimp's growth performance (Huynh, Chi, et al, ), and immune responses and disease resistance of shrimp against Vibrio alginolyticus (Huynh, Cheng, et al, ). In addition, some metabolites, including inosine monophosphate, valine and betaine in the hepatopancreas of shrimp involved in the positive effects on shrimp growth and health status were recorded by a method of 1 H nuclear magnetic resonance‐based metabolomic analyses after shrimp were fed the SYN (Huynh, Cheng, et al, ).…”

Section: Introductionmentioning

confidence: 99%

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Bacterial population in intestines of white shrimp, Litopenaeus vannamei fed a synbiotic containing Lactobacillus plantarum and galactooligosaccharide

Huynh

Chiu

et al. 2019

Aquac Res

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The aims of this study were to unravel the intestinal microbiota of Litopenaeus vannamei after being fed a diet without (control) or with the synbiotic (SYN) for 60 days using next-generation sequencing technology to see if changes in the intestinal microbiota were involved in the improved growth performance and health status of the shrimp. Next-generation sequencing data showed that six phyla, 11 classes, 19 orders, 30 families, 58 genera and 73 species with taxonomic names assigned were detected. The majority of the operational taxonomic units (OTUs) was shared between the SYN and control shrimp and comprised 37 OTUs. However, intestinal biodiversity analyses revealed that SYN-fed shrimp had a higher species richness, evenness and Shannon-Weaver index than did shrimp fed the control diet, but without reaching statistical significance. Interestingly, shrimp fed the SYN diet exhibited improved colonization of Lactobacillus plantarum and reduced prevalences of Vibrio harveyi and Photobacterium damselae in the intestines. These findings indicate that the SYN was able to modulate the intestinal bacterial community of shrimp and could be used to control vibriosis in shrimp. K E Y W O R D Sgalactooligosaccharide, intestinal microbiota, Lactobacillus plantarum, Litopenaeus vannamei, synbiotic

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Section: Introductionmentioning

confidence: 67%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Bacterial population in intestines of white shrimp, Litopenaeus vannamei fed a synbiotic containing Lactobacillus plantarum and galactooligosaccharide

Huynh

Chiu

et al. 2019

Aquac Res

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“…were predominant in shrimp intestines, accounting for 42.6% ~ 70% of the microbiota. Endemic pathogenic bacteria in shrimp intestines can be reduced when shrimp consume a symbiotic‐containing diet (Huynh et al, ), leading to an increase in disease resistance against Vibrio infections (Huynh, Cheng, Chi, Chiu, & Liu, ). In this study, shrimp in the FSBMD and AMPD groups had lower abundances of Vibrio in the intestines compared to that of shrimp in the control.…”

Section: Discussionmentioning

confidence: 99%

Intestinal microbiota of white shrimp, Litopenaeus vannamei, fed diets containing Bacillus subtilis E20‐fermented soybean meal (FSBM) or an antimicrobial peptide derived from B. subtilis E20‐FSBM

Cheng

Yeh

et al. 2019

Aquac Res

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The purpose of the present study is to profile the microfloral structure in the intestines of the white shrimp, Litopenaeus vannamei, after being fed a control diet, in comparison with the diets containing Bacillus subtilis E20‐fermented soybean meal (FSBM) or an antimicrobial peptide (AMP) isolated from B. subtilis E20‐FSBM (diets designated FSBMD and AMPD, respectively) for 60 days. Metagenomic data showed detection of eight phyla, 20 classes, 40 orders, 68 families and 96 genera. Despite no statistically significant difference, an evaluation of microbial diversity recorded higher species richness, Shannon–Weaver diversity index and evenness in the AMPD and FSBMD groups, compared to the control diet group. Venn diagrams showed that 58 of the operational taxonomic units (OTUs) were shared among the control, FSBMD and AMPD groups, but additional beneficial microbes were only found in the FSBMD and AMPD groups. In addition to the augmentation of beneficial bacteria in the FSBMD and AMPD groups, the abundance of potential pathogens, Vibrio and Flavobacterium, was lower in the gut of shrimp fed the FSBMD and AMPD. The results clearly suggest that the FSBMD and AMPD have the capability to change the microfloral structure of shrimp intestines and could be used for disease prevention in shrimp aquaculture.

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The effects of two dietary synbiotics on growth performance, hematological parameters, and nonspecific immune responses in Japanese Eel

Olowe,

Hamidoghli,

Choi

et al. 2024

J Aqua Anim Hlth

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Feed additives have attracted increased attention in aquaculture due to their ability to modulate fish gut microbiota, resulting in improved fish growth and immunity. This study assessed two synbiotics' effects in Japanese eels: Bacillus subtilis with mannooligosaccharide (MOS) and Enterococcus faecium with fructooligosaccharide (FOS). Six diets, including a control (CON), oxytetracycline (OTC), and four synbiotic diets ‐ B.subtilis at 1 × 106 and 107 CFU/g with 5 g/kg MOS (BS6MO and BS7MO) and E. faecium at 1 × 106 and 107 CFU/g with 5 g/kg FOS (EF6FO and EF7FO) ‐ were fed to triplicate groups of 20 fish averaging 6.00 ± 0.07g for eight weeks. Fish fed the BSMOS diets showed significantly higher weight gain (WG), specific growth rate (SGR), and feed efficiency (FE) compared to the CON and OTC diets (P < 0.05), but not significantly different from those fed the EFFOS diets. Weight gain, SGR of fish fed EFFOS were not significantly different from those fed all other diets (P > 0.05). Fish fed the OTC diet showed a higher mean aspartate aminotransferase (AST) level, though the difference was not statistically significant. The myeloperoxidase (MPO) activity of fish fed the BS7MO diet was significantly higher than in all other diets, and the superoxide dismutase (SOD) activity of fish fed the BS7MO diet was also significantly higher than in the EF7FO diet. Overall, the BSMOS synbiotic diets were significantly more effective than the CON diet in enhancing fish survival against Vibrio anguillarum. Our findings suggest that synbiotics can be a preferable alternative to antibiotics in aquaculture.

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A synbiotic improves the immunity of white shrimp, Litopenaeus vannamei: Metabolomic analysis reveal compelling evidence

Cited by 78 publications

References 48 publications

Bacterial population in intestines of white shrimp, Litopenaeus vannamei fed a synbiotic containing Lactobacillus plantarum and galactooligosaccharide

Bacterial population in intestines of white shrimp, Litopenaeus vannamei fed a synbiotic containing Lactobacillus plantarum and galactooligosaccharide

Intestinal microbiota of white shrimp, Litopenaeus vannamei, fed diets containing Bacillus subtilis E20‐fermented soybean meal (FSBM) or an antimicrobial peptide derived from B. subtilis E20‐FSBM

The effects of two dietary synbiotics on growth performance, hematological parameters, and nonspecific immune responses in Japanese Eel

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