A synbiotic combination of Bifidobacterium longum Bif10 and Bifidobacterium breve Bif11, isomaltooligosaccharides and finger millet arabinoxylan prevents dextran sodium sulphate induced ulcerative colitis in mice

Sharma, Shikha; Bhatia, Ruchika; Devi, Kamalini; Rawat, Anita; Singh, Sukhpal; Bhadada, Sanjay Kumar; Bishnoi, Mahendra; Sharma, Shyam Sunder; Kondepudi, Kanthi Kiran

doi:10.1016/j.ijbiomac.2023.123326

Cited by 22 publications

(8 citation statements)

References 35 publications

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“…To explore whether oral administration of B. acidifaciens can alleviate colitis, acute colitis in mice was induced by 2.5% DSS and then treated with oral B. acidifaciens . Consistent with previous studies, we confirmed that the therapeutic application of oral B. acidifaciens can alleviate DSS-induced colitis, as shown by the DAI score, colon length and histological damage ( Hu et al, 2020 ; Dong et al, 2022 ; Sharma et al, 2023 ). Similarly, oral B. acidifaciens also alleviated colonic inflammation, as exemplified by the decline in inflammatory cytokines such as IL-1β, IL-6 and TNF-α in the serum of DSS-treated mice ( Hao et al, 2021 ; Zhao et al, 2021 ; Li X. et al, 2022 ).…”

Section: Discussionsupporting

confidence: 92%

Bacteroides acidifaciens and its derived extracellular vesicles improve DSS-induced colitis

Zheng,

Zhong,

Xie

et al. 2023

Front. Microbiol.

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Introduction“Probiotic therapy” to regulate gut microbiota and intervene in intestinal diseases such as inflammatory bowel disease (IBD) has become a research hotspot. Bacteroides acidifaciens, as a new generation of probiotics, has shown beneficial effects on various diseases.MethodsIn this study, we utilized a mouse colitis model induced by dextran sodium sulfate (DSS) to investigate how B. acidifaciens positively affects IBD. We evaluated the effects ofB. acidifaciens, fecal microbiota transplantation, and bacterial extracellular vesicles (EVs) on DSS-induced colitis in mice. We monitored the phenotype of mouse colitis, detected serum inflammatory factors using ELISA, evaluated intestinal mucosal barrier function using Western blotting and tissue staining, evaluated gut microbiota using 16S rRNA sequencing, and analyzed differences in EVs protein composition derived from B. acidifaciens using proteomics to explore how B. acidifaciens has a positive impact on mouse colitis.ResultsWe confirmed that B. acidifaciens has a protective effect on colitis, including alleviating the colitis phenotype, reducing inflammatory response, and improving intestinal barrier function, accompanied by an increase in the relative abundance of B. acidifaciens and Ruminococcus callidus but a decrease in the relative abundance of B. fragilis. Further fecal bacterial transplantation or fecal filtrate transplantation confirmed the protective effect of eosinophil-regulated gut microbiota and metabolites on DSS-induced colitis. Finally, we validated that EVs derived from B. acidifaciens contain rich functional proteins that can contribute to the relief of colitis.ConclusionTherefore, B. acidifaciens and its derived EVs can alleviate DSS-induced colitis by reducing mucosal damage to colon tissue, reducing inflammatory response, promoting mucosal barrier repair, restoring gut microbiota diversity, and restoring gut microbiota balance in mice. The results of this study provide a theoretical basis for the preclinical application of the new generation of probiotics.

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Section: Discussionsupporting

confidence: 92%

Bacteroides acidifaciens and its derived extracellular vesicles improve DSS-induced colitis

Zheng,

Zhong,

Xie

et al. 2023

Front. Microbiol.

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“…The underlying mechanism may be related to the downregulation of immune-related pathways and enhancement of intestinal barrier protection. The results of this study are similar to those of previous studies on the anti-inflammatory effects of probiotics (Wickramasinghe et al, 2015;Sharma et al, 2023).…”

Section: Discussionsupporting

confidence: 90%

“…However, disadvantages of this treatment include high recurrence rates and low remission rates (Chen et al, 2022). Previous studies showed that probiotic intervention can effectively regulate intestinal inflammation and reduce the damage (Niu et al, 2023;Sharma et al, 2023). Therefore, probiotics have become an alternative for preventing and treating several diseases, such as enteritis.…”

Section: Discussionmentioning

confidence: 99%

Clostridium butyricum isolated from giant panda can attenuate dextran sodium sulfate-induced colitis in mice

Yu,

Xie,

Guo

et al. 2024

Front. Microbiol.

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ObjectiveProbiotics are beneficial to the intestinal barrier, but few studies have investigated probiotics from giant pandas. This study aims to explore the preventive effects of giant panda-derived Clostridium butyricum on dextran sodium sulfate (DSS)-induced colitis in mice.MethodsClostridium butyricum was administered to mice 14 days before administering DSS treatment to induce enteritis.ResultsClostridium butyricum B14 could more effectively prevent colitis in mice than C. butyricum B13. C. butyricum B14 protected the mouse colon by decreasing the histology index and serum interleukin-6 (IL-6) and tumor necrosis factor alpha (TNF-α) levels, which improved intestinal inflammation-related symptoms. In addition, the treatment led to the regulation of the expression of Tifa, Igkv12-89, and Nr1d1, which in turn inhibited immune pathways. The expression of Muc4, Lama3, Cldn4, Cldn3, Ocln, Zo1, Zo2, and Snai is related the intestinal mucosal barrier. 16S sequencing shows that the C. butyricum B14 significantly increased the abundance of certain intestinal probiotics. Overall, C. butyricum B14 exerted a preventive effect on colitis in mice by inhibiting immune responses, enhancing the intestinal barrier and increasing the abundance of probiotic species. Thus, C. butyricum B14 administration helps regulate the balance of the intestinal microecology. It can suppress immune pathways and enhance barrier-protective proteins.

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“…Apart from a single strain, we also discuss the effectiveness of simultaneous combinations of probiotics, including two or more strains. The mixed preparation of B. longum Bif10 and B. breve Bif11 increased anti-inflammatory marker levels and SCFAs, ameliorating symptoms of colitis in mice ( Sharma et al, 2023 ). In addition, a mixture of L. rhamnosus NCIMB 30174, L. plantarum NCIMB 30173, L. acidophilus NCIMB 30175, and Enterococcus faecium NCIMB 30176 decreased intestinal inflammation and reduced fecal calprotectin in adults with UC ( Bjarnason et al, 2019 ).…”

Section: Therapeutic Effects Of Probiotics On Ucmentioning

confidence: 99%

Probiotics for the treatment of ulcerative colitis: a review of experimental research from 2018 to 2022

et al. 2023

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Ulcerative colitis (UC) has become a worldwide public health problem, and the prevalence of the disease among children has been increasing. The pathogenesis of UC has not been elucidated, but dysbiosis of the gut microbiota is considered the main cause of chronic intestinal inflammation. This review focuses on the therapeutic effects of probiotics on UC and the potential mechanisms involved. In animal studies, probiotics have been shown to alleviate symptoms of UC, including weight loss, diarrhea, blood in the stool, and a shortened colon length, while also restoring intestinal microecological homeostasis, improving gut barrier function, modulating the intestinal immune response, and attenuating intestinal inflammation, thereby providing theoretical support for the development of probiotic-based microbial products as an adjunctive therapy for UC. However, the efficacy of probiotics is influenced by factors such as the bacterial strain, dose, and form. Hence, the mechanisms of action need to be investigated further. Relevant clinical trials are currently lacking, so the extension of animal experimental findings to clinical application requires a longer period of consideration for validation.

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A synbiotic combination of Bifidobacterium longum Bif10 and Bifidobacterium breve Bif11, isomaltooligosaccharides and finger millet arabinoxylan prevents dextran sodium sulphate induced ulcerative colitis in mice

Cited by 22 publications

References 35 publications

Bacteroides acidifaciens and its derived extracellular vesicles improve DSS-induced colitis

Bacteroides acidifaciens and its derived extracellular vesicles improve DSS-induced colitis

Clostridium butyricum isolated from giant panda can attenuate dextran sodium sulfate-induced colitis in mice

Probiotics for the treatment of ulcerative colitis: a review of experimental research from 2018 to 2022

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