A switch of N-glycosylation of proteome and secretome during differentiation of intestinal epithelial cells

Link-Lenczowski, Paweł; Jastrzębska, Martyna; Chwalenia, Katarzyna; Pierzchalska, Małgorzata; Leja-Szpak, Anna; Bonior, Joanna; Pierzchalski, Piotr; Jaworek, Jolanta

doi:10.1016/j.bbamcr.2019.118555

Cited by 9 publications

(11 citation statements)

References 58 publications

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“…Indeed, the growth phasedependent methylation seen in the archaeon S. islandicus, the growth phase-dependent pupylation seen in the bacterium Mycobacterium smegmatis, and the differentiation stage-dependent glycosylation and distinct outcomes associated with differential protein ubiquitination in Eukarya all hint that this may be the case. [74,101,104,105]…”

Section: Discussionmentioning

confidence: 99%

Modifying Post‐Translational Modifications: A Strategy Used by Archaea for Adapting to Changing Environments?

Eichler

2020

BioEssays

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In concert with the selective pressures affecting protein folding and function in the extreme environments in which they can exist, proteins in Archaea have evolved to present permanent molecular adaptations at the amino acid sequence level. Such adaptations may not, however, suffice when Archaea encounter transient changes in their surroundings. Post‐translational modifications offer a rapid and reversible layer of adaptation for proteins to cope with such situations. Here, it is proposed that Archaea further augment their ability to survive changing growth conditions by modifying the extent, position, and, where relevant, the composition of different post‐translational modifications, as a function of the environment. Support for this hypothesis comes from recent reports describing how patterns of protein glycosylation, methylation, and other post‐translational modifications of archaeal proteins are altered in response to environmental change. Indeed, adjusting post‐translational modifications as a means to cope with environmental variability may also hold true beyond the Archaea.

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Section: Discussionmentioning

confidence: 99%

Modifying Post‐Translational Modifications: A Strategy Used by Archaea for Adapting to Changing Environments?

Eichler

2020

BioEssays

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“…The conditioned media (CM) from the different available CRC cell lines is the most analyzed secretome sample. In addition to being xenograft tumor models for CRC, human colon adenocarcinoma cell lines, such as CaCo-2, LIM1215, HCT-116 and HT-29, are used as in vitro CRC model systems to analyze intracellular and secreted proteins from intestinal epithelial cells [ 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 ].…”

Section: Secretome Samplesmentioning

confidence: 99%

“…CaCo-2 spontaneously differentiates into mature enterocytes, and at confluence, forms a polarized monolayer with the specific functional features of differentiated absorptive epithelial cells [ 42 ]. LIM1215 represents a moderately differentiated CRC cell line but a metastatic one unlike CaCo-2 [ 43 ].…”

Section: Secretome Samplesmentioning

confidence: 99%

“…Recently, the study of glycosecretome has been gaining popularity because of the potential involvement of glycosylation modifications along the secretory pathway in intestinal epithelial cell differentiation. In this context, Link-Lenczowski and colleagues qualitatively and quantitatively analyzed chemical labeled-N-oligosaccharides from undifferentiated and differentiated CaCo-2 cells by using hydrophilic interaction liquid chromatography (HILIC)-high performance liquid chromatography (HPLC) and matrix-assisted laser desorption/ionization-time of flight-MS (MALDI-ToF-MS) approaches, respectively [ 42 ].…”

Section: Secretome Proteomic Approachesmentioning

confidence: 99%

“…In a recent glycosecretome study, Link-Lenczowski and colleagues highlighted the N-glycosylation changes between two differentiation stages of CaCo-2 cell line. In particular, they found an enhanced fucosylation in differentiated cells and suggested that H4N5F1 glycans might be a biomarker of intestinal epithelial cell differentiation [ 42 ].…”

Section: Biomarker Discovery For Crc Early Diagnosis By Secretome mentioning

confidence: 99%

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Secretome Proteomic Approaches for Biomarker Discovery: An Update on Colorectal Cancer

2020

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Searching for new cancer-related biomarkers is a key priority for the early detection of solid tumors, such as colorectal cancer (CRC), in clinically relevant biological fluids. The cell line and/or tumor tissue secretome represents a valuable resource for discovering novel protein markers secreted by cancer cells. The advantage of a secretome analysis is the reduction of the large dynamic range characterizing human plasma/serum, and the simultaneous enrichment of low abundance cancer-secreted proteins, thereby overcoming the technical limitations underlying the direct search in blood samples. In this review, we provided a comprehensive overview of recent studies on the CRC secretome for biomarker discovery, focusing both on methodological and technical aspects of secretome proteomic approaches and on biomarker-independent validation in CRC patient samples (blood and tissues). Secretome proteomics are mainly based on LC-MS/MS analyses for which secretome samples are either in-gel or in-solution trypsin-digested. Adequate numbers of biological and technical replicates are required to ensure high reproducibility and robustness of the secretome studies. Moreover, another major challenge is the accuracy of proteomic quantitative analysis performed by label-free or labeling methods. The analysis of differentially expressed proteins in the CRC secretome by using bioinformatic tools allowed the identification of potential biomarkers for early CRC detection. In this scenario, this review may help to follow-up the recent secretome studies in order to select promising circulating biomarkers to be validated in larger screenings, thereby contributing toward a complete translation in clinical practice.

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Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update for 2019–2020

Harvey

2023

Mass Spectrometry Reviews

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This review is the tenth update of the original article published in 1999 on the application of matrix‐assisted laser desorption/ionization (MALDI) mass spectrometry to the analysis of carbohydrates and glycoconjugates and brings coverage of the literature to the end of 2020. Also included are papers that describe methods appropriate to analysis by MALDI, such as sample preparation techniques, even though the ionization method is not MALDI. The review is basically divided into three sections: (1) general aspects such as theory of the MALDI process, matrices, derivatization, MALDI imaging, fragmentation, quantification and the use of arrays. (2) Applications to various structural types such as oligo‐ and polysaccharides, glycoproteins, glycolipids, glycosides and biopharmaceuticals, and (3) other areas such as medicine, industrial processes and glycan synthesis where MALDI is extensively used. Much of the material relating to applications is presented in tabular form. The reported work shows increasing use of incorporation of new techniques such as ion mobility and the enormous impact that MALDI imaging is having. MALDI, although invented nearly 40 years ago is still an ideal technique for carbohydrate analysis and advancements in the technique and range of applications show little sign of diminishing.

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A switch of N-glycosylation of proteome and secretome during differentiation of intestinal epithelial cells

Cited by 9 publications

References 58 publications

Modifying Post‐Translational Modifications: A Strategy Used by Archaea for Adapting to Changing Environments?

Modifying Post‐Translational Modifications: A Strategy Used by Archaea for Adapting to Changing Environments?

Secretome Proteomic Approaches for Biomarker Discovery: An Update on Colorectal Cancer

Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update for 2019–2020

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