A swine model of reproducible timed induction of peripheral arterial shunt failure: Developing warning signs of imminent shunt failure

Stonko, David P.; Patel, Neerav; Edwards, Joseph; Abdou, Hossam; Lang, E; Elansary, Noha; Treffalls, Rebecca N.; White, Joseph; Morrison, Jonathan J.

doi:10.1016/j.jvssci.2022.07.001

Cited by 7 publications

(2 citation statements)

References 31 publications

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“…We have used these techniques in swine weighing up to 90 kg. 9 , 15 , 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 If swine weighing less than 40 kg are used, we recommend imaging their external iliac artery diameter to confirm that it will support instrumentation. 29 , 30 We chose the gender of the animals based on availability and cost, and ultimately, only male animals were included in the study.…”

Section: Methodsmentioning

confidence: 99%

Technical and analytical approach to biventricular pressure-volume loops in swine including a completely endovascular, percutaneous closed-chest large animal model

Stonko,

Rousseau,

Price

et al. 2024

JVS-Vascular Science

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Section: Methodsmentioning

confidence: 99%

Technical and analytical approach to biventricular pressure-volume loops in swine including a completely endovascular, percutaneous closed-chest large animal model

Stonko,

Rousseau,

Price

et al. 2024

JVS-Vascular Science

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“…Many large animal laboratories utilize invasive techniques, such as surgical or endovascular methods, to design, assess, validate, and innovate across the breadth of the biomedical field ( 1 – 5 ). Unfortunately, during these developmental stages and in what are often low-resource animal labs, attrition is not uncommon, with studies often calculating sample size based on an expected 10% attrition rate ( 6 , 7 ).…”

Section: Introductionmentioning

confidence: 99%

Autologous blood resuscitation for large animals in a research setting using the Hemafuse device: Preliminary data of device use for controlled and real-world hemorrhage

Treffalls

Lubas²,

Morrison³

et al. 2022

Front. Vet. Sci.

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IntroductionNew low-cost technologies are needed to salvage lost blood in low-resource settings and large animal laboratories. The Hemafuse device is a simple mechanical device that can recover lost blood during surgery. The aim of this study is to assess the feasibility of this device for resuscitating large animals with controlled and unintended hemorrhage and to provide device considerations for use in this context.MethodsThis study had two experimental components: (1) the Hemafuse device was kept on-shelf and used as needed to assess real-world use for unintended hemorrhage during experiments, and (2) animals underwent a controlled hemorrhage protocol, where four anesthetized swine underwent aortic and external jugular vein catheterization for pressure monitoring. Animals were hemorrhaged into the pelvis, and the Hemafuse device was used to suction the blood through a filter and pushed into a heparinized bag for subsequent retransfusion. Blood samples were collected at baseline, hemorrhage, within the device, and post-retransfusion and laboratory tests were performed.ResultsAnimals that underwent controlled hemorrhage had a baseline mean arterial pressure of 83.6 ± 7.8 mmHg, and central venous pressure of 12.8 ± 1.9 mmHg, with expected changes throughout hemorrhage and resuscitation. Following resuscitation, pH was similar to baseline (7.39 ± 0.05 vs. 7.31 ± 0.03, p = 0.24). Lactate increased throughout the experiment with no significant differences after autotransfusion compared to baseline (2.7 ± 0.7 vs. 4.1 ± 1.4 mmol/L, p = 0.37). There were no significant changes in metabolic physiology. Compared to baseline, the hemoglobin (7.8 ± 2.4 vs. 7.3 ± 1.8 g/dL, p = 0.74), hematocrit (23% ± 6.9 vs. 21.3% ± 5.6, p = 0.71), and activated clotting time (268.5 ± 44.5 vs. 193 ± 24.6 s, p = 0.35) were similar after retransfusion. When used for unintended hemorrhage, the animals were resuscitated using the device with a mean time to retransfusion time of 128.7 ± 13.3 s and 100% survival throughout the experiment.ConclusionThe Hemafuse device is feasible and efficacious for supporting large animal resuscitation. This is preliminary evidence that the device is a low-risk and low-cost off-the-shelf option for resuscitation using autologous blood with no significant effect on physiology post-retransfusion. We recommend that research laboratories consider the Hemafuse device for emergency use, particularly for highly invasive surgical laboratories where banked blood is not readily available.

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Thoracic Endovascular Aortic RepairAcutely Augments Left Ventricular Biomechanics in An Animal Model: A Mechanism for Postoperative Heart Failure and Hypertension

Stonko

Edwards

Abdou

et al. 2023

Annals of Vascular Surgery

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A swine model of reproducible timed induction of peripheral arterial shunt failure: Developing warning signs of imminent shunt failure

Cited by 7 publications

References 31 publications

Technical and analytical approach to biventricular pressure-volume loops in swine including a completely endovascular, percutaneous closed-chest large animal model

Technical and analytical approach to biventricular pressure-volume loops in swine including a completely endovascular, percutaneous closed-chest large animal model

Autologous blood resuscitation for large animals in a research setting using the Hemafuse device: Preliminary data of device use for controlled and real-world hemorrhage

Thoracic Endovascular Aortic RepairAcutely Augments Left Ventricular Biomechanics in An Animal Model: A Mechanism for Postoperative Heart Failure and Hypertension

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