A Survival Model of <i>In Vivo</i> Partial Liver Lobe Decellularization Towards <i>In Vivo</i> Liver Engineering

Wang, An; Kuriata, Olha; Xu, Fengming; Nietzsche, Sándor; Gremse, Felix; Dirsch, Olaf; Settmacher, Utz; Dahmen, Uta

doi:10.1089/ten.tec.2019.0194

Cited by 9 publications

(7 citation statements)

References 55 publications

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“… 110 More recently, Wang et al 111 improved the surgical technique and investigated three different protocols to perform in vivo liver lobe perfusion and decellularization. 112 To promote a successful in vivo partial liver lobe decellularization, the left lateral lobe was perfused with 1% SDS and 1% Triton X-100 for 1 h or only with 1% SDS or 1% Triton X-100 for 2 h, respectively. According to the authors, in vivo decellularization with 1% SDS only for 2 h was able to promote efficient cell remotion while the main ECM proteins were preserved.…”

Section: In Vivo Liver Engineeringmentioning

confidence: 99%

“…Furthermore, they established a 7 days survival model to study in vivo liver bioengineering. 112 A summary of the studies exploring in vivo bioengineering strategies is described in Table 3 and the surgical approach is depicted in Figure 5 . This technique can be successfully translational to clinical in the future to treat liver diseases such as tumor areas in the liver without performing resection steps.…”

Section: In Vivo Liver Engineeringmentioning

confidence: 99%

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Liver scaffolds obtained by decellularization: A transplant perspective in liver bioengineering

2022

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Liver transplantation is the only definitive treatment for many diseases that affect this organ, however, its quantity and viability are reduced. The study of liver scaffolds based on an extracellular matrix is a tissue bioengineering strategy with great application in regenerative medicine. Collectively, recent studies suggest that liver scaffold transplantation may assist in reestablishing hepatic function in preclinical diseased animals, which represents a great potential for application as a treatment for patients with liver disease in the future. This review focuses on useful strategies to promote liver scaffold transplantation and the main open questions about this context. We outline the current knowledge about ex vivo bioengineered liver transplantation, including the surgical techniques, recipient survival time, scaffold preparation before transplantation, and liver disease models. We also highlight the current limitations and future directions regarding in vivo bioengineering techniques.

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Section: In Vivo Liver Engineeringmentioning

confidence: 99%

Section: In Vivo Liver Engineeringmentioning

confidence: 99%

Liver scaffolds obtained by decellularization: A transplant perspective in liver bioengineering

2022

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“… 124 – 126 In addition to recellularization, other concerns such as the potential risk of xenogeneic/allogeneic livers, long-term stability of liver grafts in vivo, and bioengineering of a physiologically relevant sized liver construct should also be addressed before clinical application of the decellularized liver scaffold-based approach. 7 , 33 , 34 Wang et al 153 successfully achieved in vivo decellularization of partial liver lobes in rats with a good survival rate through the perfusion of 1% SDS solution. Although recellularization and anti-coagulation processes still require further optimization, the study represents a promising strategy for liver engineering and regeneration in vivo.…”

Section: Discussionmentioning

confidence: 99%

Stem cell therapy and tissue engineering strategies using cell aggregates and decellularized scaffolds for the rescue of liver failure

et al. 2021

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Liver failure is a lethal condition with hepatocellular dysfunction, and liver transplantation is presently the only effective treatment. However, due to the limited availability of donors and the potential immune rejection, novel therapeutic strategies are actively sought to restore the normal hepatic architectures and functions, especially for livers with inherited metabolic dysfunctions or chronic diseases. Although the conventional cell therapy has shown promising results, the direct infusion of hepatocytes is hampered by limited hepatocyte sources, poor cell viability, and engraftment. Hence, this review mainly highlights the role of stem cells and progenitors as the alternative cell source and summarizes the potential approaches based on tissue engineering to improve the delivery efficiency of cells. Particularly, the underlying mechanisms for cell therapy using stem cells and progenitors are discussed in two main aspects: paracrine effect and cell differentiation. Moreover, tissue-engineering approaches using cell aggregates and decellularized liver scaffolds for bioengineering of functional hepatic constructs are discussed and compared in terms of the potential to replicate liver physiological structures. In the end, a potentially effective strategy combining the premium advantages of stem cell aggregates and decellularized liver scaffolds is proposed as the future direction of liver tissue engineering and regeneration.

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“…Other considerations are important to maintain graft patency. For example, collagenous fibers from the vascular basement membrane that are exposed to blood flow activate the clotting cascade during extracorporeal blood perfusion or after in vivo transplantation ( Kipshidze et al, 2004 ; Caralt et al, 2014 ; Wang et al, 2020 ). The surface charge of graft materials is a pivotal reason why grafts are prone to thrombosis.…”

Section: Passivation Methods and Anticoagulants For Revascularized Bi...mentioning

confidence: 99%

Re-Endothelialization of Decellularized Liver Scaffolds: A Step for Bioengineered Liver Transplantation

Tharwat

Larson

et al. 2022

Front. Bioeng. Biotechnol.

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Bioengineered livers (BELs) are an attractive therapeutic alternative to address the donor organ shortage for liver transplantation. The goal of BELs technology aims at replacement or regeneration of the native human liver. A variety of approaches have been proposed for tissue engineering of transplantable livers; the current review will highlight the decellularization-recellularization approach to BELs. For example, vascular patency and appropriate cell distribution and expansion are critical components in the production of successful BELs. Proper solutions to these components of BELs have challenged its development. Several strategies, such as heparin immobilization, heparin-gelatin, REDV peptide, and anti-CD31 aptamer have been developed to extend the vascular patency of revascularized bioengineered livers (rBELs). Other novel methods have been developed to enhance cell seeding of parenchymal cells and to increase graft functionality during both bench and in vivo perfusion. These enhanced methods have been associated with up to 15 days of survival in large animal (porcine) models of heterotopic transplantation but have not yet permitted extended survival after implantation of BELs in the orthotopic position. This review will highlight both the remaining challenges and the potential for clinical application of functional bioengineered grafts.

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A Survival Model of In Vivo Partial Liver Lobe Decellularization Towards In Vivo Liver Engineering

Cited by 9 publications

References 55 publications

Liver scaffolds obtained by decellularization: A transplant perspective in liver bioengineering

Liver scaffolds obtained by decellularization: A transplant perspective in liver bioengineering

Stem cell therapy and tissue engineering strategies using cell aggregates and decellularized scaffolds for the rescue of liver failure

Re-Endothelialization of Decellularized Liver Scaffolds: A Step for Bioengineered Liver Transplantation

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