A survival Kit for pancreatic beta cells: stem cell factor and c-Kit receptor tyrosine kinase

Feng, Zhichao; Riopel, Matthew; Popell, Alex; Wang, Rennian

doi:10.1007/s00125-015-3504-0

Cited by 25 publications

(25 citation statements)

References 103 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The c-Kit receptor is the product of the c-kit proto-oncogene and belongs to the receptor tyrosine kinase (RTK) superfamily, with the members of this family being the cardinal regulators of cellular fate in the mammalian body (17). As an important member of the type III RTK family, it has a highly specific and restricted expression pattern, with prominent c-Kit levels expressed on the surface of hematopoietic cells, mast cells (MCs) and interstitial cells of Cajal (ICC) (18,19).…”

Section: Biological Functions Of the Scf/c-kit Systemmentioning

confidence: 99%

Role of stem cell growth factor/c-Kit in the pathogenesis of irritable bowel syndrome

Chai

Huang

Tang

et al. 2017

Experimental and Therapeutic Medicine

View full text Add to dashboard Cite

Irritable bowel syndrome (IBS) is a functional bowel disease with a complicated etiopathogenesis, often characterized by gastrointestinal motility disorder and high visceral sensitivity. IBS is a comprehensive multi-systemic disorder, with the interaction of multiple factors, such as mental stress, intestinal function and flora, heredity, resulting in the disease. The existence of a common mechanism underlying the aforementioned factors is currently unknown. The lack of therapies that comprehensively address the disease symptoms, including abdominal pain and diarrhea, is a limitation of current IBS management. The current review has explored the role of the SCF/c-Kit receptor/ligand system in IBS. The SCF/c-Kit system constitutes a classical ligand/receptor tyrosine kinase signaling system that mediates inflammation and smooth muscle contraction. Additionally, it provides trophic support to neural crest-derived cell types, including the enteric nervous system and mast cells. The regulation of SCF/c-Kit on the interstitial cells of Cajal (ICC) suggest that it may play a key role in the aberrant intestinal dynamics and high visceral sensitivity observed in IBS. The role of the SCF/c-Kit system in intestinal motility, inflammation and nerve growth has been reported. From the available biomedical evidence on the pathogenesis of IBS, it has been concluded that the SCF-c-Kit system is a potential therapeutic target for rational drug design in the treatment of IBS.

show abstract

Section: Biological Functions Of the Scf/c-kit Systemmentioning

confidence: 99%

Role of stem cell growth factor/c-Kit in the pathogenesis of irritable bowel syndrome

Chai

Huang

Tang

et al. 2017

Experimental and Therapeutic Medicine

View full text Add to dashboard Cite

show abstract

“…The c-Kit receptor is the product of c-Kit proto-oncogene, belonging to the receptor tyrosine kinase (RTK) superfamily, which is the main regulator of mammalian cell fate (24). c-Kit is an important member of the type III RTK family, is highly specific, and has a restricted expression pattern, expressed in hematopoietic cells, mast cells, and on the surface of the ICC (25,26).…”

Section: Discussionmentioning

confidence: 99%

Phenotypic changes of interstitial cells of Cajal after intestinal obstruction in rat model

Zou

Zhou

et al. 2019

Braz J Med Biol Res

View full text Add to dashboard Cite

The objective was to study the effect of mechanical intestinal obstruction in rats on the phenotype of interstitial cells of Cajal (ICC). Healthy Wistar rats were randomly divided into sham-operation group (C), one day obstruction group (M1), two days obstruction group (M2), and three days obstruction group (M3), with 10 rats in each group. The expression of SCF mRNA and c-Kit protein in intestinal tissue was investigated by RT-PCR and immunohistochemistry. Compared with the sham-operation group, the relative expression of SCF mRNA and the expression of c-Kit protein in intestinal tissue were significantly decreased in both obstruction groups. Levels decreased gradually with the prolongation of obstruction time, and significantly decreased on the 3rd day after obstruction (P<0.05). Immunohistochemical staining of the small intestine showed that the number of ICC in the sham-operation group was the highest, and they were gradually decreased with the extension of obstruction time in the M1 to M3 groups. There was a significant difference between groups (P<0.05). Intestinal obstruction caused a decrease in the concentrations of SCF mRNA and c-Kit protein in ICC. With the prolongation of intestinal obstruction, the number of ICCs gradually decreased.

show abstract

“…SCF and its receptor c-kit play an essential role in hemopoiesis and maintaining hematopoietic stem cell survival, mast cell stimulation, mediating the function of the pancreas, and melanogenesis [ 23 ]. A mutation in SCF could cause anemia, lack of mast cells, immaturity and loss of functions of pancreatic endocrine cells, and disappearance of pigmentation, as well as the absence of melanocytes [ 24 , 25 ]. c-Kit is a receptor tyrosine kinase expressed on melanocytes.…”

Section: Introductionmentioning

confidence: 99%

Silencing Stem Cell Factor Gene in Fibroblasts to Regulate Paracrine Factor Productions and Enhance c-Kit Expression in Melanocytes on Melanogenesis

Liu

Chang

et al. 2018

IJMS

View full text Add to dashboard Cite

Melanogenesis is a complex physiological mechanism involving various paracrine factors. Skin cells such as keratinocytes, fibroblasts, and melanocytes communicate with one another through secreted regulators, thereby regulating the melanocytes’ bio-functions. The stem cell factor (SCF) is a paracrine factor produced by fibroblasts, and its receptor, c-kit, is expressed on melanocytes. Binding of SCF to c-kit activates autophosphorylation and tyrosine kinase to switch on its signal transmission. SCF inhibition does not suppress fibroblast proliferation in MTT assay, and SCF silencing induced mRNA expressions of paracrine factor genes, HGF, NRG-1, and CRH in qPCR results. Following UVB stimulation, gene expressions of HGF, NRG, and CRH were higher than homeostasis; in particular, HGF exhibited the highest correlation with SCF variations. We detected fibroblasts regulated SCF in an autocrine-dependent manner, and the conditioned medium obtained from fibroblast culture was applied to treat melanocytes. Melanogenesis-related genes, tyrosinase and pmel17, were upregulated under conditioned mediums with SCF silencing and exposed to UVB treatments. Melanin quantities in the melanocytes had clearly increased in the pigment content assay. In conclusion, SCF silencing causes variations in both fibroblast paracrine factors and melanocyte melanogenesis, and the differences in gene expressions were observed following UVB exposure.

show abstract

A survival Kit for pancreatic beta cells: stem cell factor and c-Kit receptor tyrosine kinase

Cited by 25 publications

References 103 publications

Role of stem cell growth factor/c-Kit in the pathogenesis of irritable bowel syndrome

Role of stem cell growth factor/c-Kit in the pathogenesis of irritable bowel syndrome

Phenotypic changes of interstitial cells of Cajal after intestinal obstruction in rat model

Silencing Stem Cell Factor Gene in Fibroblasts to Regulate Paracrine Factor Productions and Enhance c-Kit Expression in Melanocytes on Melanogenesis

Contact Info

Product

Resources

About