A survey of viral diseases of livestock characterized by skin lesions in Kanam Local Government Area of Plateau State, Nigeria

Gambo, Panzam; Maguda, A.S.; Adole, J.A.; Dyek, D.Y.; Ifende, V.I.; Bot, Christopher; Adedeji, Adeyinka Jeremy

doi:10.4314/nvj.v39i3.8

Cited by 6 publications

(10 citation statements)

References 16 publications

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“…Interestingly, farmers that participated in the study claimed LSD is as important as FMD probably due to the impact of the disease on their herds. In previous studies in Nigeria, majority of cattle herders/pastoralists were familiar with LSD and the common name of the disease amongst transhumance herders is 'Bollo' which shows the disease may be endemic, but neglected (Gambo et al, 2018). Over all morbidity of 16% and mortality rate of 2% was obtained in this study which is higher than the report of Adedeji et al (2018) with morbidity rate of 6.25%, but mortality rate of 2.5%.…”

Section: Discussioncontrasting

confidence: 51%

“…Lumpy skin disease is endemic but neglected disease of cattle in Nigeria. This study was designed in response to repeated outbreaks of (Gambo et al, 2018;Ifende et al, 2019). The study identifies the epidemiological features and economic impact of LSD on subsistence smallholder cattle farmers in the study area.…”

Section: Discussionmentioning

confidence: 99%

“…Briefly, the reaction mixture consisted of 14.0 µl of H2O, 2.5 µl 10× taq buffer with (NH4)SO4, 3.0µl MgCl2, 0.5µl dNTPs, 1.0µl of each primer, 0.5µl Taq DNA polymerase and 2.5µl DNA template. The PCR conditions were as follows: 94℃ for 2min initial denaturation, followed by 35 cycles of 94℃ for 40s, 55℃ for 30s, 72℃ for 30s and final elongation at 72℃ for 5min (Adedeji et al, 2018). The electrophoresis of PCR products was done on 1.5% agarose gel stained with ethidium bromide at 100V for 30min and a 100bp ladder (New England Biolabs) was used as DNA marker.…”

Section: Laboratory Analysismentioning

confidence: 99%

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Epidemiological features of lumpy skin disease outbreaks amongst herds of cattle in Bokkos, north-central Nigeria

Atai¹,

Olaolu²,

Adole³

et al. 2021

Sokoto J. Vet. Sc.

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Lumpy Skin Disease (LSD) is a severe viral transboundary disease of mostly cattle caused by LSD Virus (LSDV). This epidemiological survey of LSD amongst herds of cattle in Bokkos Local Government Area (LGA) of North Central Nigeria was carried out in 2019 as a response to farmers’ reports of repeated outbreaks of LSD in their herds of cattle. A focused group discussion with cattle farmers purposefully selected was used for the disease investigation and data collection. Twelve skin scab samples were collected from suspected cases within the study area. The viral attachment protein gene of the LSDV was amplified using polymerase chain reaction (PCR). Analysis of the focus group discussion revealed that all farmers interviewed practiced extensive farm management system and claimed that their animals shared same communal water points and grazing area. Furthermore, 47% (7/15) of the farmers have experienced LSD twice in their herds, while 27% (4/15) have had the outbreak thrice on their farms. The morbidity rates of LSD were 3% – 49% and mortality rates were 1% – 6%. Sixty percent of farmers claimed that incidence of LSD is related to season of the year. All farmers sell off their sick animals in the livestock market and confirmed LSD affects market price of their animals. PCR results revealed that in 91.6% (11/12) samples analysed, LSDV was detected. This study confirms LSD outbreaks based on PCR result and clinical signs and symptoms in Butura, Daffo and Kunduk of Bokkos LGA, North Central Nigeria.

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Section: Discussioncontrasting

confidence: 51%

Section: Discussionmentioning

confidence: 99%

Section: Laboratory Analysismentioning

confidence: 99%

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Epidemiological features of lumpy skin disease outbreaks amongst herds of cattle in Bokkos, north-central Nigeria

Atai¹,

Olaolu²,

Adole³

et al. 2021

Sokoto J. Vet. Sc.

Self Cite

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“…Skin lesions can be clinically misdiagnosed as other cutaneous diseases. Economically, poxvirus diseases cause losses due to damage to the skin, decreased milk and meat production and trade restrictions in addition to morbidity and mortality [ 3 , 4 ].…”

Section: Introductionmentioning

confidence: 99%

First molecular characterization of poxviruses in cattle, sheep, and goats in Botswana

Modise

Settypalli

Kgotlele

et al. 2021

Virol J

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Background Poxviruses within the Capripoxvirus, Orthopoxvirus, and Parapoxvirus genera can infect livestock, with the two former having zoonotic importance. In addition, they induce similar clinical symptoms in common host species, creating a challenge for diagnosis. Although endemic in the country, poxvirus infections of small ruminants and cattle have received little attention in Botswana, with no prior use of molecular tools to diagnose and characterize the pathogens. Methods A high-resolution melting (HRM) assay was used to detect and differentiate poxviruses in skin biopsy and skin scab samples from four cattle, one sheep, and one goat. Molecular characterization of capripoxviruses and parapoxviruses was undertaken by sequence analysis of RPO30 and GPCR genes. Results The HRM assay revealed lumpy skin disease virus (LSDV) in three cattle samples, pseudocowpox virus (PCPV) in one cattle sample, and orf virus (ORFV) in one goat and one sheep sample. The phylogenetic analyses, based on the RPO30 and GPCR multiple sequence alignments showed that the LSDV sequences of Botswana were similar to common LSDV field isolates encountered in Africa, Asia, and Europe. The Botswana PCPV presented unique features and clustered between camel and cattle PCPV isolates. The Botswana ORFV sequence isolated from goat differed from the ORFV sequence isolated from sheep. Conclusions This study is the first report on the genetic characterization of poxvirus diseases circulating in cattle, goats, and sheep in Botswana. It shows the importance of molecular methods to differentially diagnose poxvirus diseases of ruminants.

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“…Lesions on the skin of the affected animals can be clinically confused with other cutaneous diseases. Economically, pox virus diseases cause losses due to damage to the skin, decreased milk and meat production and trade restriction in addition to morbidity and mortality (3,4).…”

Section: Introductionmentioning

confidence: 99%

First Molecular Characterization of Poxviruses in Cattle, Sheep, and Goats in Botswana

Modise

Settypalli²,

Kgotlele

et al. 2021

Preprint

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BackgroundPoxviruses within the Capripoxvirus, Orthopoxvirus, and Parapoxvirus genera can infect livestock, with the two former presenting also zoonotic importance. In addition, they induce similar clinical symptoms in common host species, creating a challenge for diagnosis. Although endemic in the country, poxvirus infections of small ruminants and cattle have received little attention in Botswana, and there was no prior attempt of using molecular tools to diagnose the diseases and characterize the pathogens.MethodsA high-resolution melting (HRM) assay was used to detect and differentiate poxviruses in samples from four cattle (from Mahalapye, Kasane, and Molepolole), one sheep (from Jwaneng), and one goat (from Kasane). Molecular characterization of capripoxviruses and parapoxviruses was undertaken by sequence analysis of RPO30 and GPCR genes.ResultsThe HRM assay revealed lumpy skin disease virus (LSDV) in three cattle samples, pseudocowpox virus (PCPV) in one cattle sample, and orf virus (ORFV) in one goat and one sheep sample. The phylogenetic analyses, based on the RPO30 and GPCR, and the inspection of the multiple sequence alignments showed that the LSDV sequences of Botswana were more like those of common LSDV field isolates encountered in Africa, Asia, and Europe. The Botswana PCPV presented unique features and clustered between camel isolates and cattle isolates of the PCPV group. The Botswana ORFV from goat differed from the ORFV collected in sheep.ConclusionsThis study is the first report on the genetic characterization of pox virus diseases circulating in cattle, goats, and sheep in Botswana. It shows the importance of molecular methods to differentially diagnose pox virus diseases of ruminants.

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A survey of viral diseases of livestock characterized by skin lesions in Kanam Local Government Area of Plateau State, Nigeria

Cited by 6 publications

References 16 publications

Epidemiological features of lumpy skin disease outbreaks amongst herds of cattle in Bokkos, north-central Nigeria

Epidemiological features of lumpy skin disease outbreaks amongst herds of cattle in Bokkos, north-central Nigeria

First molecular characterization of poxviruses in cattle, sheep, and goats in Botswana

First Molecular Characterization of Poxviruses in Cattle, Sheep, and Goats in Botswana

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