In this paper, we discuss a relationship between the chirality of knots and higher-dimensional twisted Alexander polynomials associated with holonomy representations of hyperbolic 3-cone-manifolds. In particular, we provide a new necessary condition for a knot, that appears in a hyperbolic 3-cone-manifold of finite volume as a singular set, to be amphicheiral. Moreover, we can detect the chirality of hyperbolic twist knots, according to our criterion, using low-dimensional irreducible representations.