A survey of more than 90 commercially available luminometers and imaging devices for low‐light measurements of chemiluminescence and bioluminescence, including instruments for manual, automatic and specialized operation, for HPLC, LC, GLC and microtitre plates. Part 1: Descriptions

Abstract

This survey was compiled in January and February 1992 from information available in public domain literature requested by and supplied to the author by numerous companies in the previous two months. More than 90 luminometers (manual, automatic, microtitre plate, HPLC, LC, GLC, imaging and specials) from more than 60 companies are included. Each company was invited to supply company brochures, technical details, user manual and information about software and any other information concerning their product(s). The response varied from a single information sheet to promotional material and up to full product information and specification with technical details, user manuals and scientific publications. Where an instrument is dedicated to a single task the company may have only provided details relevant to accomplishing that task. Part 2 of this survey will contain photographs of some of the luminometers. It is intended that updates to this review will be published at least annually in this journal and suppliers are invited to provide full technical details of new luminometric equipment to the author.

Plant calcium signaling and monitoring: pros and cons and recent experimental approaches

“…Therefore an extremely sensitive light detector device, such as photomultiplier tubes (Electron Tubes, Ruislip, U.K.) or photon-counting cameras (Scienceware, Pequannock, N.J., U.S.A.; Photek, St. Leonardson-Sea, U.K.; Berthold Detection Systems, Pforzheim, Federal Republic of Germany; Hamamatsu Photonics) (Creton et al 1999;Aikens 1999;Stanley 1992Stanley , 1999, is needed. The spectral properties of the bioluminescent material have to be in accordance with the spectral sensitivity of the detector and photon-counting mode should be used in order to maximize the signalto-noise ratio Wright 1999, Tomkins andLyons 1999).…”

Recombinant aequorin methods for measurement of intracellular calcium in plants

“…The simplest system is that in which the output is directed to a chart recorder, and changes in luminescence are plotted during the experiment. A wide range of models of off-the-shelf chemiluminometers is available [15] or they can be assembled from the component parts, such as those available from Thorn EM!. If choosing a photomultiplier tube, it should be selected for maximum efficiency in the blue light wavelength range, it should have a low dark current (i.e.…”

5-HT2 receptor blocker sarpogrelate prevents downregulation of antiapoptotic protein Bcl-2 and protects the heart against ischemia–reperfusion injury

“…For the analysis of myocardial ATP, a left ventricular specimen from the risk area was isolated, frozen in the liquid nitrogen and processed for the analysis of ATP levels as described earlier (Stanley, 1992). In brief, the frozen samples were crushed and the cells lysed using Tris hydrochloride and EDTA buffer, pH 7.4.…”