“…The three bacterial strains were grown in a constant temperature shaker (orbital shaking) at 170 rpm for 20-48 h at 37 °C. [23][24][25][26][27][28][29][30][31][32][33][34] The bacterial solution cultured to an absorbance of 0.8-1 A (600 nm) was diluted 1000 times with sterile dilution water. When the absorbance was between 0.8 and 1.0 (600 nm), the bacteria were generally in the middle of the exponential growth phase, during which bacterial activity was high and the accuracy of bacterial testing could be ensured.…”
This research investigated the potential use of 5-chloro-2-methyl-4-isothiazolin-3-one (CMIT) as a biocide in aircraft fuel systems, which is rarely studied due to the unique properties of such systems.
“…The three bacterial strains were grown in a constant temperature shaker (orbital shaking) at 170 rpm for 20-48 h at 37 °C. [23][24][25][26][27][28][29][30][31][32][33][34] The bacterial solution cultured to an absorbance of 0.8-1 A (600 nm) was diluted 1000 times with sterile dilution water. When the absorbance was between 0.8 and 1.0 (600 nm), the bacteria were generally in the middle of the exponential growth phase, during which bacterial activity was high and the accuracy of bacterial testing could be ensured.…”
This research investigated the potential use of 5-chloro-2-methyl-4-isothiazolin-3-one (CMIT) as a biocide in aircraft fuel systems, which is rarely studied due to the unique properties of such systems.
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