A Survey of Maltese Grapevine Germplasm Using SSR Markers

Giannetto, Sabrina; Caruana, Randall; Notte, Pierfederico La; Costacurta, Angelo; Crespan, Manna

doi:10.5344/ajev.2010.61.3.419

Cited by 6 publications

(2 citation statements)

References 13 publications

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“…In Greece, traditionally ampelographic descriptors, which are based on the comparison of their morphology were used [ 4 , 5 ] until the discovery of DNA-based markers such as Random Amplified Length Polymorphism (RAPD [ 6 ]), Inter Simple Sequence Repeats (ISSR, [ 7 ]) and Simple Sequence Repeats (SSR, [ 8 , 9 , 10 ]). SSR markers have become the preferred markers for the standardization and analysis of genetic variation with regard to grapevine genetic resources, as numerous studies prove [ 2 , 3 , 9 , 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 ]. Studies use SSR in order to distinguish grape cultivars, for example, in Cyprus molecular genotyping analysis using 11 SSR markers allowed the accurate identification and discrimination of a set of autochthonous cultivars, clarifying their relationship with Greek, Bulgarian, and western European Vitis genetic material [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

“…Studies use SSR in order to distinguish grape cultivars, for example, in Cyprus molecular genotyping analysis using 11 SSR markers allowed the accurate identification and discrimination of a set of autochthonous cultivars, clarifying their relationship with Greek, Bulgarian, and western European Vitis genetic material [ 13 ]. Similarly, DNA typing at 13 SSR loci identified 28 different genotypes comprising mainly indigenous germplasm cultivated in the archipelago of Malta [ 14 ] contributing to the accurate identification of unknown or neglected grapevine germplasm in the region. Genotyping with 23 SSR markers assessed the genetic diversity of Moroccan grape accessions as compared to the Maghreb and European gene pools [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

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Analysis of Wine-Producing Vitis vinifera L. Biotypes, Autochthonous to Crete (Greece), Employing Ampelographic and Microsatellite Markers

Avramidou

Masaoutis

Pitsoli

et al. 2023

Life

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Vitis vinifera ssp. vinifera (domesticated grapevine) includes thousands of cultivars, which are classified according to their main uses, as wines, fresh fruits or dried raisins and sultanas since ancient times. Evidence showed that Crete grapevine cultivars and winemaking date back to 2300 BC. In this study, fifty-one genotypes belonging to seven different traditional Vitis vinifera cultivars, presumed autochthonous to the island of Crete, were selected for their wine-producing potential and classified by 51 ampelographic descriptors. In addition, five genotypes belonging to two non-autochthonous cultivars were included as out-group controls. Subsequently, in order to characterize genetic diversity, establish genetic relationships within and between cultivars and solve accession-labeling problems, genotypes were fingerprinted employing Simple Sequence Repeat (SSR or microsatellite) markers. Four of the autochthonous cultivars namely ‘Vidiano’, ‘Vilana’, ‘Plyto’, and ‘Moschato Spinas’ are used in the local economy for blanc (white) wine production while the rest, namely ‘Kotsifali’, ‘Liatiko’ and ‘Mantilari’ for Noir (red) wines. The two cultivars employed as out-group were ‘Moschato Samou’ and ‘Moschato Alexandrias’: both white wine producers. Ampelography-based clustering grouped the majority of genotypes along cultivar-specific clusters. All three Moschato cultivars formed a distinct clade pointing to the non-autochthonous origin of ‘Moschato Spinas’. A total of one hundred and thirteen (113) SSR alleles were amplified from thirteen (13) SSR loci, with an average number of alleles per locus equal to 10.23 revealing ample genetic polymorphism. The cumulative probability of identity was also quite high (3.389 × 10−16). The overall observed heterozygosity was 0.837 while for twenty-nine of the examined genotypes, at least one private SSR allele was detected. The majority of genotypes were grouped in cultivar-specific clusters. The results of this paper pave the way for the certification and registration of clones of some of the most important wine-producing cultivars in Crete.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Analysis of Wine-Producing Vitis vinifera L. Biotypes, Autochthonous to Crete (Greece), Employing Ampelographic and Microsatellite Markers

Avramidou

Masaoutis

Pitsoli

et al. 2023

Life

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Constructing a genetic linkage map of Vitis aestivalis-derived “Norton” and its use in comparing Norton and Cynthiana

et al. 2017

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Zibibbo Nero Characterization, a Red-Wine Grape Revertant of Muscat of Alexandria

et al. 2014

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Muscat of Alexandria is known in Italy as Zibibbo. Zibibbo nero, red-wine grapes, is a sport mutation of Zibibbo variety. A biochemical and molecular characterization of berry colour (VvMybA1 and VvMybA2 genes, Vitis vinifera MYeloBlastosis) and aroma Muscat (VvDXS gene, 1-deoxy-D-xylulose 5-phosphate synthase) traits in both Zibibbo cultivars was performed, as well as ampelographic and genetic identification analyses. Molecular investigations were performed also for two putative Zibibbo parents (Moscato Bianco and Triboto), in order to prove the white-to-red shift of the red-skinned mutant. Ampelographic and genetic analysis demonstrated the high similarity between Zibibbo and Zibibbo nero, as well as a comparable aroma profile, characterized mainly by high content of linalool, geranic acid and geraniol (about 70 %). The Zibibbo nero anthocyanin profile was characterized by a high proportion in cyanidin-3-O-glucoside (about 69.23 %). The molecular characterization of VvMybA1 and VvMybA2 locus detected non-functional alleles for white-skinned samples, while also the functional alleles were observed for red-skinned samples. About the VvDXS locus, the aromatic varieties showed the typical pattern of Muscat variety, while Triboto (Zibibbo parent) showed the non-Muscat-like flavour pattern. The colour locus structure of Zibibbo and its putative parents suggested that Zibibbo nero is a berry colour revertant of Zibibbo.

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A Survey of Maltese Grapevine Germplasm Using SSR Markers

Cited by 6 publications

References 13 publications

Analysis of Wine-Producing Vitis vinifera L. Biotypes, Autochthonous to Crete (Greece), Employing Ampelographic and Microsatellite Markers

Analysis of Wine-Producing Vitis vinifera L. Biotypes, Autochthonous to Crete (Greece), Employing Ampelographic and Microsatellite Markers

Constructing a genetic linkage map of Vitis aestivalis-derived “Norton” and its use in comparing Norton and Cynthiana

Zibibbo Nero Characterization, a Red-Wine Grape Revertant of Muscat of Alexandria

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