A survey of jaagsiekte sheep retrovirus (JSRV) infection in sheep in the three northeastern provinces of China

Shi, Wen; Jia, Shijie; Guan, Xiaohong; Yao, Xin; Pan, Ronghui; Huang, Xinning; Ma, Yingying; Wei, Jing; Xu, Yigang

doi:10.1007/s00705-020-04919-6

Cited by 6 publications

(5 citation statements)

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“…Ovine pulmonary adenocarcinoma (OPA), also known as pulmonary adenomatosis and jaagsiekte, is a contagious neoplasia derived from type II pneumocytes and club cells, that occurs in sheep and, exceptionally, in goats and mouflons. The disease is associated with high economic losses not only due to the related mortality but also due to the lack of a vaccine or methods of early detection of the disease [1,2].…”

Section: Introductionmentioning

confidence: 99%

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The Diagnostic Challenges of Ovine Pulmonary Adenocarcinoma

et al. 2021

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Ovine pulmonary adenocarcinoma (OPA), also known as sheep pulmonary adenomatosis and jaagsiekte, is a contagious pulmonary tumor of sheep, characterized by neoplastic proliferation of type II pneumocyte and club cells. OPA is induced by the oncogenic activity of the envelope glycoprotein (Env) of exogenous jaagsiekte sheep retrovirus (JSRV). This disease is associated with significant economic losses in numerous sheep raising countries. The onset of suggestive clinical signs is often late, making difficult the early diagnosis of the disease and timely implementation of control measures on the affected farms. Further, the lack of diagnostic tests that can be performed routinely by veterinary clinicians to accurately assess infected animals (e.g., serological or others) means that the true prevalence at flock level is not known. Imaging diagnostic methods (e.g., ultrasound, X-ray and computed tomography) can be used to support the clinical diagnosis, even in pre-clinical stages in affected flocks. The diagnosis must be confirmed by PCR of nasal excretions or immunohistochemistry and PCR of tumor lesions. No vaccine for OPA has yet been developed. Thus, in this work, we review the main methods of diagnosis of OPA in order to support the clinician in the identification of the disease, avoid underdiagnosis and allow the implementation of suitable measures to prevent and control its spread.

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Section: Introductionmentioning

confidence: 99%

“…The disease is described around the world in many sheep farming areas, including countries on the European and Asian, African and American continents [1,[5][6][7][8]. In Portugal, several sporadic cases have been reported, although the prevalence is not known [9].…”

Section: Introductionmentioning

confidence: 99%

The Diagnostic Challenges of Ovine Pulmonary Adenocarcinoma

et al. 2021

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“…The JSRV genome is uniquely characterized by its native envelope structural protein. The active oncogene consists of the cytoplasmic domain of the transmembrane glycoprotein and as well as, other cytoplasmic domains found in the surface glycoprotein [32]. Even though other regions of the envelope protein have also been implicated in transformation, the cytoplasmic tail of the envelope transmembrane protein is a crucial feature [11,14,33,34].…”

Section: Discussionmentioning

confidence: 99%

A novel strategy for developing vaccine candidate against Jaagsiekte sheep retrovirus from the envelope and gag proteins: an in-silico approach

2022

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Background Sheep pulmonary adenocarcinoma (OPA) is a contagious lung cancer of sheep caused by the Jaagsiekte retrovirus (JSRV). OPA typically has a serious economic impact worldwide. A vaccine has yet to be developed, even though the disease has been globally spread, along with its complications. This study aimed to construct an effective multi-epitopes vaccine against JSRV eliciting B and T lymphocytes using immunoinformatics tools. Results The designed vaccine was composed of 499 amino acids. Before the vaccine was computationally validated, all critical parameters were taken into consideration; including antigenicity, allergenicity, toxicity, and stability. The physiochemical properties of the vaccine displayed an isoelectric point of 9.88. According to the Instability Index (II), the vaccine was stable at 28.28. The vaccine scored 56.51 on the aliphatic index and -0.731 on the GRAVY, indicating that the vaccine was hydrophilic. The RaptorX server was used to predict the vaccine's tertiary structure, the GalaxyWEB server refined the structure, and the Ramachandran plot and the ProSA-web server validated the vaccine's tertiary structure. Protein-sol and the SOLPro servers showed the solubility of the vaccine. Moreover, the high mobile regions in the vaccine’s structure were reduced and the vaccine’s stability was improved by disulfide engineering. Also, the vaccine construct was docked with an ovine MHC-1 allele and showed efficient binding energy. Immune simulation remarkably showed high levels of immunoglobulins, T lymphocytes, and INF-γ secretions. The molecular dynamic simulation provided the stability of the constructed vaccine. Finally, the vaccine was back-transcribed into a DNA sequence and cloned into a pET-30a ( +) vector to affirm the potency of translation and microbial expression. Conclusion A novel multi-epitopes vaccine construct against JSRV, was formed from B and T lymphocytes epitopes, and was produced with potential protection. This study might help in controlling and eradicating OPA.

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“…Exogenous (exJSRV) and endogenous (enJSRV) retroviruses can be distinguished from one another by the presence of the ScaI restriction site in the Gag gene and the "LHMKYXXM" motif in the envelope protein. However, the ScaI restriction site is found in oncogenic JSRVs but absent in endogenous retroviruses in sheep, and the "LHMKYXXM" pattern is found in all exJSRVs but not in enJSRVs (Shi et al 2021). The whole genome sequencing analysis of JSRV from India revealed a close relationship with China and USA isolates (Mishra et al 2018).…”

Section: B Cmentioning

confidence: 98%

Ovine pulmonary adenocarcinoma (OPA) in sheep: an update on epidemiology, pathogenesis and diagnosis

B N,

K N

et al. 2023

J Exp Bio & Ag Sci

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Ovine pulmonary adenocarcinoma (OPA) is a spontaneous lung tumor in sheep caused by Jaagsiekte sheep retrovirus (JSRV) belonging to the Retroviridae. The primary aim of this review work is to give brief insights into the epidemiological aspects of OPA based on a meta-analysis of available research work. This review article also discussed pathogenesis, diagnostic tests and control strategies available for OPA in Sheep. This will help in developing future strategies for disease-free status in India. This disease is endemic in Europe, Africa, Asia, and American continents, causing significant economic losses due to chronic respiratory illness and persistent infections in flocks. The virus is unique among retroviruses with selective affinity to lungs and is the only virus known to cause spontaneous lung tumors in sheep. The incubation time ranges for sheep with naturally occurring OPA ranged from one to four years. There are two pathological forms of the disease: classical and atypical. At an early stage, OPA is difficult to detect in sheep due to a lack of preclinical diagnostic methods, as JSRV is poorly immunogenic and doesn't induce an immune response. PCR, histopathology, and immunohistochemistry are recommended methods for OIE diagnosis. To become a JSRV-free country, mandatory surveillance, detection, and removal of positive animals are required, as OPA is difficult to control due to a lack of vaccines and preclinical diagnostic tests. Due to its similar histological and molecular pathogenesis to that of human lung cancer, OPA is considered an ideal large animal model of human lung adenocarcinoma.

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A survey of jaagsiekte sheep retrovirus (JSRV) infection in sheep in the three northeastern provinces of China

Cited by 6 publications

References 35 publications

The Diagnostic Challenges of Ovine Pulmonary Adenocarcinoma

The Diagnostic Challenges of Ovine Pulmonary Adenocarcinoma

A novel strategy for developing vaccine candidate against Jaagsiekte sheep retrovirus from the envelope and gag proteins: an in-silico approach

Ovine pulmonary adenocarcinoma (OPA) in sheep: an update on epidemiology, pathogenesis and diagnosis

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