Introduction: The progress of research in the field of dental stem cells is at a high pace. Various studies have shown oral tissues to be a good source for adult stem cells and therefore dentists can play a great role in the field of regenerative therapies. For the dentists to explore the different applications of oral stem cells, they should be aware of the basic biology and storing and processing of stem cells. The aim of this study was to assess the knowledge, attitude, and practice regarding the applications of stem cells in dentistry, among the interns and dental teaching faculty members of two dental colleges in Ernakulam, Kerala, India. Materials and methods: A cross-sectional questionnaire-based survey was carried out, consisting of 16 questions, to assess the objectives of the study. The study was conducted among dental house surgeons, postgraduate students, and teaching faculty members of two dental colleges in Ernakulam district, Kerala, India. For each question, the results were expressed as a number and percentage of responses and inferential statistical analysis was done using chi-square test. Results: Almost majority of the dentists (84%) never received any training on handling and processing of oral stem cells, but about 89% were interested in attending training workshops. The majority (77.9%) were willing to practice regenerative dentistry. Conclusion: Dentists are supportive for using stem cell-based regenerative treatments in their practice and are interested in attending more training for the same. Most of the dentists feel that the undergraduate syllabus should include this topic.