A survey of cereals, cereal products, feedstuffs and porcine kidneys for ochratoxin a by radioimmunoassay

Fukal, L.

doi:10.1080/02652039009373889

Cited by 22 publications

(8 citation statements)

References 14 publications

(8 reference statements)

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“…Their conclusion was that OTA is actually ingested and present in serum. Fukal (1990) [67] reported that, based on measurements using the same kit, food and feed may contain OTA, but it was not detected in porcine kidney.…”

Section: Immunoassay Formatsmentioning

confidence: 99%

Immunochemical Methods for Ochratoxin A Detection: A Review

Meulenberg¹

2012

Toxins

111

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The safety of food and feed depends to a great deal on quality control. Numerous compounds and organisms may contaminate food and feed commodities and thus pose a health risk for consumers. The compound of interest in this review is ochratoxin A (OTA), a secondary metabolite of the fungi Aspergillus and Penicillium . Due to its adverse health effects, detection and quantification are of utmost importance. Quality control of food and feed requires extraction and analysis, including TLC, HPLC, MS, and immunochemical methods. Each of these methods has its advantages and disadvantages. However, with regard to costs and rapidity, immunochemical methods have gained much interest in the last decade. In this review an introduction to immunochemistry and assay design will be given to elucidate the principles. Further, the application of the various formats to the detection and quantification of ochratoxin will be described, including the use of commercially available kits.

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Section: Immunoassay Formatsmentioning

confidence: 99%

Immunochemical Methods for Ochratoxin A Detection: A Review

Meulenberg¹

2012

Toxins

111

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“…However, P. verrucosum is The able technical assistance of Ms Anne Hinsby and Ms mostly considered as a fungus associated with spoilage of Azar Amiri-Rad is greatly appreciated. stored grain, with the subsequent production of ochratoxin (Fukal 1990). Penicillium nalgiovense is known as a starter in…”

Section: ------------------------------------------------------------mentioning

confidence: 99%

Ecophysiological characterization of common food-borne fungi in relation to pH and water activity under various atmospheric compositions

Haasum

Nielsen

1998

J Appl Microbiol

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I . H AA S UM AN D P. V. N IE LS E N. 1998. The combined effects of pH, water activity (a w ), oxygen (O 2 ) and carbon dioxide (CO 2 ) levels on growth and sporulation of 10 common food-borne fungi were studied. The use of a multivariate statistical method (PLS) for the analysis of data showed that the fungi could be grouped according to their physiological response to changes in the four tested factors. Carbon dioxide, a w and pH were found to be the most significant factors describing differences and similarities among the fungi. Maximal inhibitory effect of elevated levels of CO 2 (5-25%) and decreased a w (0·99-0·95) varied among the 10 species from 6 to 77% and from 52 to 100%, respectively. Sporulation of the fungi was sensitive to all tested factors. Furthermore, interaction of CO 2 and a w displayed a significant effect on sporulation. It was shown that different fungal species associated with the same ecosystem responded similarly to changes in the tested factors. Thus, fungi which are not phylogenetically related may be physiologically related or show a common strategy of life.

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“…ELISA methods have been applied to analysis of barley and other grains, feed, animal tissues and serum as referenced in reviews by Chu (1992) and Wilson et al (1998). Radioimmunoassay for OTA has been applied to surveys of cereals, cereal products, feedstuffs, and pig serum and tissues (Fukal, 1990(Fukal, , 1991Rousseau et aI., 1986) but does not appear to have been used in recent years. Examples of detection limits that can be obtained with commercially available ELISA kits and ELISAs developed inhouse are shown in Table 2; in general these were lower where cleanup was used.…”

Section: Enzyme-linked Immunosorbent Assay (Elisa)mentioning

confidence: 98%

Methods of Analysis for Ochratoxin A

Scott

2002

Advances in Experimental Medicine and Biology

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The mycotoxin ochratoxin A (OT A) is produced by the fungi Aspergillus alutaceus and Penicillium verrucosum and has carcinogenic, nephrotoxic, teratogenic and immunosuppressive properties. The levels of OT A in foodstuffs are regulated in several countries, so reliable and sensitive methods are necessary for its determination. Procedures for extraction ofOTA from ground foods generally use an organic solvent in the presence of acid or an extraction solvent containing aqueous sodium bicarbonate. Cleanup procedures include partition into aqueous sodium bicarbonate, solid phase extraction (SPE) columns and immunoaffinity chromatography. The latter technique allows detection of sub-ppb levels of OTA in a wide variety of foods and in plasma. The most widely used determinative procedure is reversed phase liquid chromatography (LC) with detection by fluorescence (excitation 330-340 nm, emission 460-470 nm) or, more recently, by tandem mass spectrometry. ELISA methods are also available. Certified reference materials containing OT A have been prepared.

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A survey of cereals, cereal products, feedstuffs and porcine kidneys for ochratoxin a by radioimmunoassay

Cited by 22 publications

References 14 publications

Immunochemical Methods for Ochratoxin A Detection: A Review

Immunochemical Methods for Ochratoxin A Detection: A Review

Ecophysiological characterization of common food-borne fungi in relation to pH and water activity under various atmospheric compositions

Methods of Analysis for Ochratoxin A

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