A survey of apparent blood volumes and sample geometries among filter paper bloodspot samples submitted for lead screening

Peck, Heather R.; Timko, Denise M.; Landmark, James D.; Stickle, Douglas F.

doi:10.1016/j.cca.2008.10.020

Cited by 26 publications

(13 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Education on blood spotting is critical to obtain DBS samples of adequate quality. Peck et al () determined the apparent blood volume of each DBS in 422 DBS from 138 patients. Calculations relied on a linear regression between the spotted volume of blood of standard samples and corresponding DBS areas (see also Section II.C.4).…”

Section: Blood Collectionmentioning

confidence: 99%

The use of mass spectrometry to analyze dried blood spots

Wagner

Tonoli

Varesio

et al. 2014

Mass Spectrometry Reviews

221

228

View full text Add to dashboard Cite

Dried blood spots (DBS) typically consist in the deposition of small volumes of capillary blood onto dedicated paper cards. Comparatively to whole blood or plasma samples, their benefits rely in the fact that sample collection is easier and that logistic aspects related to sample storage and shipment can be relatively limited, respectively, without the need of a refrigerator or dry ice. Originally, this approach has been developed in the sixties to support the analysis of phenylalanine for the detection of phenylketonuria in newborns using bacterial inhibition test. In the nineties tandem mass spectrometry was established as the detection technique for phenylalanine and tyrosine. DBS became rapidly recognized for their clinical value: they were widely implemented in pediatric settings with mass spectrometric detection, and were closely associated to the debut of newborn screening (NBS) programs, as a part of public health policies. Since then, sample collection on paper cards has been explored with various analytical techniques in other areas more or less successfully regarding large‐scale applications. Moreover, in the last 5 years a regain of interest for DBS was observed and originated from the bioanalytical community to support drug development (e.g., PK studies) or therapeutic drug monitoring mainly. Those recent applications were essentially driven by improved sensitivity of triple quadrupole mass spectrometers. This review presents an overall view of all instrumental and methodological developments for DBS analysis with mass spectrometric detection, with and without separation techniques. A general introduction to DBS will describe their advantages and historical aspects of their emergence. A second section will focus on blood collection, with a strong emphasis on specific parameters that can impact quantitative analysis, including chromatographic effects, hematocrit effects, blood effects, and analyte stability. A third part of the review is dedicated to sample preparation and will consider off‐line and on‐line extractions; in particular, instrumental designs that have been developed so far for DBS extraction will be detailed. Flow injection analysis and applications will be discussed in section IV. The application of surface analysis mass spectrometry (DESI, paper spray, DART, APTDCI, MALDI, LDTD‐APCI, and ICP) to DBS is described in section V, while applications based on separation techniques (e.g., liquid or gas chromatography) are presented in section VI. To conclude this review, the current status of DBS analysis is summarized, and future perspectives are provided. © 2014 Wiley Periodicals, Inc. Mass Spec Rev 35:361–438, 2016.

show abstract

Section: Blood Collectionmentioning

confidence: 99%

The use of mass spectrometry to analyze dried blood spots

Wagner

Tonoli

Varesio

et al. 2014

Mass Spectrometry Reviews

221

228

View full text Add to dashboard Cite

show abstract

“…A caveat to interpretation of our findings is that our experiments were performed on laboratory-produced specimens, using bolus-addition of a fixed volume of blood. In clinical practice, bloodspots may exhibit considerable variation in apparent volume, and may be formed by multiple drop additions [13]. Such variations might affect the relationship between α(R) and α(P).…”

Section: Discussionmentioning

confidence: 99%

Estimation of hematocrit in filter paper dried bloodspots by potassium measurement: advantage of use of perimeter ring samples over circular center sub-punch samples

Rufail

McCloskey

Stickle

2016

Clinical Chemistry and Laboratory Medicine (CCLM)

Self Cite

View full text Add to dashboard Cite

show abstract

“…After protein precipitation using ZnSO 4 (including addition of an isotope-labeled internal standard), vortexing and centrifugation, the supernatants were injected into a 2D HPLC system and onto an online extraction column. Online column extraction using high flows of 5 ml/min on conventional pre-column cartridges using a simple 6-port switching valve for the analysis of tacrolimus have been described before 47 . The mobile phase was chosen so that tacrolimus and its internal standard concentrated in the front of the extraction column and did not migrate over the column during the cleanup step.…”

Section: Discussionmentioning

confidence: 99%

Quantification of the Immunosuppressant Tacrolimus on Dried Blood Spots Using LC-MS/MS

Shokati

Bodenberger

Gadpaille

et al. 2015

JoVE

View full text Add to dashboard Cite

The calcineurin inhibitor tacrolimus is the cornerstone of most immunosuppressive treatment protocols after solid organ transplantation in the United States. Tacrolimus is a narrow therapeutic index drug and as such requires therapeutic drug monitoring and dose adjustment based on its whole blood trough concentrations. To facilitate home therapeutic drug and adherence monitoring, the collection of dried blood spots is an attractive concept. After a finger stick, the patient collects a blood drop on filter paper at home. After the blood is dried, it is mailed to the analytical laboratory where tacrolimus is quantified using high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) in combination with a simple manual protein precipitation step and online column extraction.For tacrolimus analysis, a 6-mm disc is punched from the saturated center of the blood spot. The blood spot is homogenized using a bullet blender and then proteins are precipitated with methanol/0.2 M ZnSO 4 containing the internal standard D 2, 13 C-tacrolimus. After vortexing and centrifugation, 100 µl of supernatant is injected into an online extraction column and washed with 5 ml/min of 0.1 formic acid/acetonitrile (7:3, v:v) for 1 min. Hereafter, the switching valve is activated and the analytes are back-flushed onto the analytical column (and separated using a 0.1% formic acid/acetonitrile gradient). Tacrolimus is quantified in the positive multi reaction mode (MRM) using a tandem mass spectrometer.The assay is linear from 1 to 50 ng/ml. Inter-assay variability (3.6%-6.1%) and accuracy (91.7%-101.6%) as assessed over 20 days meet acceptance criteria. Average extraction recovery is 95.5%. There are no relevant carry-over, matrix interferences and matrix effects. Tacrolimus is stable in dried blood spots at RT and at +4 °C for 1 week. Extracted samples in the autosampler are stable at +4 °C for at least 72 hr. Video LinkThe video component of this article can be found at

show abstract

A survey of apparent blood volumes and sample geometries among filter paper bloodspot samples submitted for lead screening

Cited by 26 publications

References 11 publications

The use of mass spectrometry to analyze dried blood spots

The use of mass spectrometry to analyze dried blood spots

Estimation of hematocrit in filter paper dried bloodspots by potassium measurement: advantage of use of perimeter ring samples over circular center sub-punch samples

Quantification of the Immunosuppressant Tacrolimus on Dried Blood Spots Using LC-MS/MS

Contact Info

Product

Resources

About