A Surgical Device to Study the Efficacy of Bioengineered Skin Substitutes in Mice Wound Healing Models

Jeschke, Marc G.; Sadri, Ali-Reza; Belo, Cassandra; Amini-Nik, Saeid

doi:10.1089/ten.tec.2016.0545

Cited by 18 publications

(17 citation statements)

References 30 publications

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“…The mice were divided into four groups (10 mice in each group) and were used to evaluate the biodegradability and biocompatibility of keratin scaffolds on excisional wound on Days 3, 6, 9, and 12. To establish wound models, two full‐thickness excisional wounds (8 mm in size) were created on the back skin using biopsy punch (Chen, Mirza, Kwon, DiPietro, & Koh, ; Jeschke, Sadri, Belo, & Amini‐Nik, ). Mitomycin C (1 mg/ml) was applied to the wound area for 5–10 min to prevent wound healing.…”

Section: Methodsmentioning

confidence: 99%

Keratin scaffolds with human adipose stem cells: Physical and biological effects toward wound healing

Lin

Chen

Tang

et al. 2019

J Tissue Eng Regen Med

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Keratin, a natural biomaterial derived from wool or human hair, has the intrinsic ability to interact with different types of cells and the potential to serve as a controllable extracellular matrix that can be used a scaffold for tissue engineering. In this study, we demonstrated a simple and fast technique to construct 3D keratin scaffolds for accelerated wound healing using a lyophilization method based on extraction of keratin from human hair. The physical properties of the keratin scaffolds such as water uptake, pore size, and porosity can be adjusted by changing the protein concentrations during the fabrication process. The keratin scaffolds supported human adipose stem cells (hASCs) adhesion, proliferation, and differentiation. In vivo study performed on ICR mice showed that keratin scaffolds with hASCs shortened skin wound healing time, accelerated epithelialization, and promoted wound remodeling. Therefore, keratin scaffolds alone or together with hASCs may serve as therapeutic agents for repairing wounded tissue.

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Section: Methodsmentioning

confidence: 99%

Keratin scaffolds with human adipose stem cells: Physical and biological effects toward wound healing

Lin

Chen

Tang

et al. 2019

J Tissue Eng Regen Med

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“…The two types of domes used in the study were either perforated or non‐perforated as previously shown (Jeschke et al. ). The domes are used to prevent wound healing in order to test the effects of the grafted skin substitutes alone.…”

Section: Methodsmentioning

confidence: 99%

“…The preparation of wound was performed as previously described (Jeschke et al. ). Briefly, the mice were anesthetized with 1–2% isofluorane + oxygen until unconscious, and the eyes were protected using eye gel.…”

Section: Methodsmentioning

confidence: 99%

“…Masson's trichrome staining was performed for histological studies as previously described (Jeschke et al. ). Immunohistochemistry was performed to characterize the cellular composition of the harvested wounds.…”

Section: Methodsmentioning

confidence: 99%

“…We have previously described and validated a surgical device, a dome, that is used to prevent skin contraction while allowing the housing of a synthetic skin graft (Jeschke et al. ). When using skin substitutes on wounds in humans, cells and other factors from the surrounding intact skin and the wound bed infiltrate the skin substitute for wound closure (Poon et al.…”

Section: Introductionmentioning

confidence: 99%

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Examining the contribution of surrounding intact skin during cutaneous healing

2019

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Severe cutaneous wounds expose the body to the external environment, which may lead to impairments in bodily functions and increased risk of infection. There is a need to develop skin substitutes which could effectively promote complete skin regeneration following an injury. Murine models are used to test such skin substitutes, but their healing involves contraction of the dermis not found in human wounds. We have previously described a device called a dome, which comes in two models, that is used to prevent skin contraction in mice. One model provides a physical barrier to minimize contraction, and the other model has additional perforations in the barrier to allow cellular contribution from the surrounding intact skin. Taking advantage of an enhanced version of these two models, we compared granulation tissue formation, the extent of vascularization, and the transition to myofibroblastic phenotype between the models. We enhanced the dome by developing a twist open cap dome and applied the two models of the dome into the excisional wound biopsy in mice. We demonstrate that the dome can be used to prevent skin contraction in mice. The control model prevented skin contraction while barricading the contribution of surrounding intact skin. When not barricaded, the intact skin enhances wound healing by increasing the number of myofibroblasts and neovascularization. Using a novel model of inhibition of skin contraction in rodents, we examined the contribution from the surrounding intact skin to granulation tissue formation, myofibroblastic differentiation, and neovascularization during the course of skin healing in mice.

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Biomaterials for Skin Substitutes

Sheikholeslam

Wright

Jeschke

et al. 2017

Adv Healthcare Materials

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159

126

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Patients with extensive burns rely on the use of tissue engineered skin due to a lack of sufficient donor tissue, but it is a challenge to identify reliable and economical scaffold materials and donor cell sources for the generation of a functional skin substitute. The current review attempts to evaluate the performance of the wide range of biomaterials available for generating skin substitutes, including both natural biopolymers and synthetic polymers, in terms of tissue response and potential for use in the operating room. Natural biopolymers display an improved cell response, while synthetic polymers provide better control over chemical composition and mechanical properties. It is suggested that not one material meets all the requirements for a skin substitute. Rather, a composite scaffold fabricated from both natural and synthetic biomaterials may allow for the generation of skin substitutes that meet all clinical requirements including a tailored wound size and type, the degree of burn, the patient age, and the available preparation technique. This review aims to be a valuable directory for researchers in the field to find the optimal material or combination of materials based on their specific application.

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A Surgical Device to Study the Efficacy of Bioengineered Skin Substitutes in Mice Wound Healing Models

Cited by 18 publications

References 30 publications

Keratin scaffolds with human adipose stem cells: Physical and biological effects toward wound healing

Keratin scaffolds with human adipose stem cells: Physical and biological effects toward wound healing

Examining the contribution of surrounding intact skin during cutaneous healing

Biomaterials for Skin Substitutes

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