A surfactin cyclopeptide of WH1fungin used as a novel adjuvant for intramuscular and subcutaneous immunization in mice

Gao, Ziyan; Wang, Shengying; Qi, Gaofu; Pan, Hui; Zhang, Li; Zhou, Xiaohui; Liu, Jingjing; Zhao, Xiuyun; Wu, Jie

doi:10.1016/j.peptides.2012.08.021

Cited by 20 publications

(23 citation statements)

References 23 publications

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“…We recently showed that Surfactin (SFN), a natural lipopeptide produced by Bacillus , can be used both as parenteral and non-parenteral adjuvant for various protein or peptide antigens78. SFN is a kind of non-pathogen-associated molecular patterns that helps to elicit a balanced Th1/Th2 response in vivo 7.…”

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confidence: 99%

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Surfactin inducing mitochondria-dependent ROS to activate MAPKs, NF-κB and inflammasomes in macrophages for adjuvant activity

Gan

Gao

Zhao

et al. 2016

Sci Rep

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Surfactin, a natural lipopeptide, can be used both as parenteral and non-parenteral adjuvant for eliciting immune response. However, the mechanisms that confer its adjuvant properties have not been fully explored. By staining with NHS-Rhodamine B labeled surfactin and Mito-Tracker Green, we found surfactin could penetrate into macrophages to bind with mitochondria, following induce ROS that could be inhibited by mitochondria-dependent ROS inhibitor. ROS enhanced p38 MAPK and JNK expression, as well their phorsphorylation, following activated NF-κB nuclear translocation in macrophages that was obviously inhibited by mitochondria-dependent ROS inhibitor. However, inhibition of ROS production only weakened p38 MAPK and JNK expression, but not their phosphorylation in macrophages. As a result, surfaction could activate NF-κB to release TNF-α by the mitochondria-dependent ROS signalling pathway. ROS also induced macrophages apoptosis to release endogenous danger signals, following activated inflammasomes of NLRP1, NLRP3, IPAF and AIM2 in vitro and only NLRP1 in vivo, as well caspase-1 and IL-1 in macrophages, which were significantly inhibited by pre-treatment with ROS inhibitors. Collectively, surfactin as a kind of non-pathogen-associated molecular patterns, modulates host innate immunity by multiple signalling pathways, including induction of mitochondria-dependent ROS, activating MAPKs and NF-κB, and inducing cell apoptosis to realease endogenous danger signals for activation of inflammasomes.

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confidence: 99%

“…SFN is a kind of non-pathogen-associated molecular patterns that helps to elicit a balanced Th1/Th2 response in vivo 7. Notably, the mucosal adjuvant activity of SFN is comparable to cholera toxin, the “gold standard” of mucosal adjuvanticity8.…”

mentioning

confidence: 99%

Surfactin inducing mitochondria-dependent ROS to activate MAPKs, NF-κB and inflammasomes in macrophages for adjuvant activity

Gan

Gao

Zhao

et al. 2016

Sci Rep

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“…WH1fungin can act as an immunoadjuvant inducing Th2-predominant immunity [17,18]. Thus, we deduced that oral WH1fungin could induce a Th2-bias immune response for suppression of autoimmunity.…”

Section: Discussionmentioning

confidence: 93%

“…In vivo assay also showed surfactin can reduce the acute inflammation of septic shock induced by lipopolysaccharide (LPS) in rats [15]. We isolated and characterized a WH1fungin surfactin secreted by B. amyloliquefaciens WH1 with immunoadjuvant activities, which can induce a Th2-predominant immune response in vivo [16][17][18]. Based on anti-inflammation role of surfactin, we detect whether WH1fungin could inhibit chronic T1DM in this study.…”

Section: Introductionmentioning

confidence: 91%

“…The total IgG, IgG1 and IgG2a titers in serum were determined by an end-point ELISA assay [17,18]. Briefly, the mice serum were serially diluted from 1:100 for coating ELISA plates at 100 l per well, blocked with 2% bovine serum albumin (BSA), reacted with horseradish peroxidase (HRP) labeled rabbit anti-mouse IgG, IgG1 or IgG2a antibodies (Invitrogen, USA), developed with 3,3 ,5,5 -tetramethylbenzidine (TMB) substrate, and then the reaction was stopped by addition of 50 l, 2 M H 2 SO 4 for detecting OD 450nm value by a microplate reader (BioTek, USA).…”

Section: Determination Of Blood Glucose Serum Insulin and Antibodiesmentioning

confidence: 99%

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Immunomodulation therapy of diabetes by oral administration of a surfactin lipopeptide in NOD mice

Gao

Zhao

Yang

et al. 2014

Vaccine

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Mast cells partially contribute to mucosal adjuvanticity of surfactin in mice

Yoshino

Takeshita

Kawamura

et al. 2017

Immunity Inflam & Disease

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IntroductionSurfactin (SF) is a cyclic lipopeptide that has potent mucosal adjuvant properties. However, immunological mechanisms of SF adjuvant action have not yet been elucidated. As some cyclic lipopeptides, such as polymyxin, can stimulate histamine release from mast cells, we hypothesized that mast cell activation is critical for SF adjuvanticity.Methods/ResultsWe observed that following intranasal immunization with ovalbumin (OVA) plus SF, the titers of the OVA‐specific antibody (Ab) in the mucosal secretions and plasma of mast cell‐deficient mice were significantly lower than those in congenic normal mice, although OVA‐specific Ab did not entirely disappear from mast cell‐deficient mice. SF induced degranulation of mast cells and release of histamine in vitro. To investigate whether SF stimulated mast cells in vivo, we measured body temperature of mice immunized intranasally with OVA plus SF because histamine level affects body temperature. Following immunizations, body temperature of immunized congenic normal mice transiently decreased, whereas body temperature of mast cell‐deficient mice did not change. Plasma levels of OVA‐specific IgE Ab were not significantly different in mast cell‐deficient and congenic normal mice. These findings suggest that SF directly affected mast cells in an IgE Ab‐independent fashion. Furthermore, we analyzed the effects of SF on MC/9 mast cells cultured in vitro. MC/9 cells stimulated by SF released not only histamine but also leukotriene B4 and prostaglandin D2. Moreover, SF up‐regulated mRNA expression levels of Tnf, Ccr5, and Il4 genes in mast cells. These cytokines may play a facilitating role in OVA‐specific immune responses in mice.ConclusionOverall, our results showed that mast cell activation partially mediated SF adjuvanticity.

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A surfactin cyclopeptide of WH1fungin used as a novel adjuvant for intramuscular and subcutaneous immunization in mice

Cited by 20 publications

References 23 publications

Surfactin inducing mitochondria-dependent ROS to activate MAPKs, NF-κB and inflammasomes in macrophages for adjuvant activity

Surfactin inducing mitochondria-dependent ROS to activate MAPKs, NF-κB and inflammasomes in macrophages for adjuvant activity

Immunomodulation therapy of diabetes by oral administration of a surfactin lipopeptide in NOD mice

Mast cells partially contribute to mucosal adjuvanticity of surfactin in mice

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