A Surface‐Modified Dendrimer Set for Potential Application as Drug Delivery Vehicles: Synthesis, In Vitro Toxicity, and Intracellular Localization

Fuchs, Sabine; Kapp, Timo; Otto, Henning; Schöneberg, Torsten; Franke, P.; Gust, Ronald; Schlüter, A. Dieter

doi:10.1002/chem.200305386

Cited by 106 publications

(106 citation statements)

References 98 publications

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“…20 Figure 1 also shows that the viability of MCF-7/ADR cells is higher than that of MCF-7 cells at the high concentration of PAMAM-NH 2 (200-1,000 μg/mL). Figure 2 shows that the uptake rate of PAMAM-NH 2 in MCF-7/ADR cells was lower than that in MCF-7 cells.…”

mentioning

confidence: 87%

The cellular uptake mechanism, intracellular transportation, and exocytosis of polyamidoamine dendrimers in multidrug-resistant breast cancer cells

Zhang¹,

Liú²,

Zhang³

et al. 2016

IJN

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Polyamidoamine dendrimers, which can deliver drugs and genetic materials to resistant cells, are attracting increased research attention, but their transportation behavior in resistant cells remains unclear. In this paper, we performed a systematic analysis of the cellular uptake, intracellular transportation, and efflux of PAMAM-NH 2 dendrimers in multidrug-resistant breast cancer cells (MCF-7/ADR cells) using sensitive breast cancer cells (MCF-7 cells) as the control. We found that the uptake rate of PAMAM-NH 2 was much lower and exocytosis of PAMAM-NH 2 was much greater in MCF-7/ADR cells than in MCF-7 cells due to the elimination of PAMAM-NH 2 from P-glycoprotein and the multidrug resistance-associated protein in MCF-7/ADR cells. Macropinocytosis played a more important role in its uptake in MCF-7/ADR cells than in MCF-7 cells. PAMAM-NH 2 aggregated and became more degraded in the lysosomal vesicles of the MCF-7/ADR cells than in those of the MCF-7 cells. The endoplasmic reticulum and Golgi complex were found to participate in the exocytosis rather than endocytosis process of PAMAM-NH 2 in both types of cells. Our findings clearly showed the intracellular transportation process of PAMAM-NH 2 in MCF-7/ADR cells and provided a guide of using PAMAM-NH 2 as a drug and gene vector in resistant cells.

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confidence: 87%

The cellular uptake mechanism, intracellular transportation, and exocytosis of polyamidoamine dendrimers in multidrug-resistant breast cancer cells

Zhang¹,

Liú²,

Zhang³

et al. 2016

IJN

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“…68 The cytotoxicity of MCF-7 breast cancer cells was examined in vitro using low-generation (G0, G1, and G2) PAMAM-like polymers. 69 However, dendrimers-drug conjugate has an antineoplastic agent and is covalently attached to the peripheral groups of the dendrimers, and has distinct advantages over drugencapsulated systems. For local delivery in breast cancer, doxorubicin-G4-PAMAM complexes were encapsulated into the liposomes.…”

Section: Dendrimersmentioning

confidence: 99%

Drug delivery approaches for breast cancer

Singh

Lillard

et al. 2017

IJN

174

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Breast cancer is one of the most common cancers affecting women worldwide. The controlled release of drugs to the precise site of the disease using a nanocarrier vehicle increases the therapeutic efficiency of the drugs. Nanotechnology-based approaches used to endorse clinical improvement from a disease also help to understand the interaction of malignant cells with their microenvironment. Receptor-based targeting is another approach for drug delivery which is undergoing clinical trials. Nanoparticles (NPs) delivery has been proven to promise high loading capacity, less toxicity, and stability of the drugs or biomolecules compared to traditional chemotherapeutic drugs. The goal of this review is to present the current problems of breast cancer therapy and discuss the NP-based targeting to overcome the hurdles of conventional drug therapy approach.

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“…Secondly, the tert-butyl ester terminal obtained opens the possibility to hydrolyze them and obtain carboxylic acids as noncytotoxic peripheral groups. 63 Once dendrons 161 and 162 were synthesized, the focal point -OH was transformed into the iodide (compounds 164 and 165) with I2 in the presence of imidazole and…”

Section: Page 233mentioning

confidence: 99%