A Supramolecular Sensing Platform for Phosphate Anions and an Anthrax Biomarker in a Microfluidic Device

Eker, Bilge; Yilmaz, M. Deniz; Schlautmann, Stefan; Gardeniers, Han; Huskens, Jurriaan

doi:10.3390/ijms12117335

Cited by 25 publications

(10 citation statements)

References 70 publications

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“…Overall, compared to reported conventional ELISAs for anthrax spore detection [4,22], PHAD has improved sensitivity, robustness and/or substantially reduced run times. Nucleic acid sequence (DNA or RNA), dipicolinic acid (DPA), and surface resonance (Raman spectroscopy)-based methods of anthrax spore detection all require sample processing procedures (often including sample enrichment) and equipment limiting the portability and practicality in the field [6,23,24,25]. …”

Section: Discussionmentioning

confidence: 99%

Rapid Detection of Bacillus anthracis Spores Using Immunomagnetic Separation and Amperometry

Waller¹,

Hew²,

Holdaway³

et al. 2016

Biosensors

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Portable detection and quantitation methods for Bacillus anthracis (anthrax) spores in pure culture or in environmental samples are lacking. Here, an amperometric immunoassay has been developed utilizing immunomagnetic separation to capture the spores and remove potential interferents from test samples followed by amperometric measurement on a field-portable instrument. Antibody-conjugated magnetic beads and antibody-conjugated glucose oxidase were used in a sandwich format for the capture and detection of target spores. Glucose oxidase activity of spore pellets was measured indirectly via amperometry by applying a bias voltage after incubation with glucose, horseradish peroxidase, and the electron mediator 2,2′-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid). Target capture was mediated by polyclonal antisera, whereas monoclonal antibodies were used for signal generation. This strategy maximized sensitivity (500 target spores, 5000 cfu/mL), while also providing a good specificity for Bacillus anthracis spores. Minimal signal deviation occurs in the presence of environmental interferents including soil and modified pH conditions, demonstrating the strengths of immunomagnetic separation. The simultaneous incubation of capture and detection antibodies and rapid substrate development (5 min) result in short sample-to-signal times (less than an hour). With attributes comparable or exceeding that of ELISA and LFDs, amperometry is a low-cost, low-weight, and practical method for detecting anthrax spores in the field.

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Section: Discussionmentioning

confidence: 99%

Rapid Detection of Bacillus anthracis Spores Using Immunomagnetic Separation and Amperometry

Waller¹,

Hew²,

Holdaway³

et al. 2016

Biosensors

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“…Sensor arrays that mimic the human sensory system (i.e., artificial nose or tongue) have been used to discriminate between multiple analytes. For example, Eker et al, reported a supramolecular luminescent sensor platform with five parallel sensing self-assembled monolayers incorporated in a microfluidic device that can detect multiple analytes (phosphate anions and aromatic carboxylic acids) ( Eker et al, 2011 ). Other examples that involve supramolecular arrays for differentially sensing biomolecular analytes used antibody-free systems for the detection of the histone code ( Minaker et al, 2012 ) and gold nanoclusters for nucleotide sensing ( Rana et al, 2012 ; Pezzato et al, 2013 ), and even mammalian cell types or cancer states ( Bajaj et al, 2010 ).…”

Section: Emerging Trends and Current Challengesmentioning

confidence: 99%

Evolution of Supramolecular Systems Towards Next-Generation Biosensors

2021

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Supramolecular materials, which rely on dynamic non-covalent interactions, present a promising approach to advance the capabilities of currently available biosensors. The weak interactions between supramolecular monomers allow for adaptivity and responsiveness of supramolecular or self-assembling systems to external stimuli. In many cases, these characteristics improve the performance of recognition units, reporters, or signal transducers of biosensors. The facile methods for preparing supramolecular materials also allow for straightforward ways to combine them with other functional materials and create multicomponent sensors. To date, biosensors with supramolecular components are capable of not only detecting target analytes based on known ligand affinity or specific host-guest interactions, but can also be used for more complex structural detection such as chiral sensing. In this Review, we discuss the advancements in the area of biosensors, with a particular highlight on the designs of supramolecular materials employed in analytical applications over the years. We will first describe how different types of supramolecular components are currently used as recognition or reporter units for biosensors. The working mechanisms of detection and signal transduction by supramolecular systems will be presented, as well as the important hierarchical characteristics from the monomers to assemblies that contribute to selectivity and sensitivity. We will then examine how supramolecular materials are currently integrated in different types of biosensing platforms. Emerging trends and perspectives will be outlined, specifically for exploring new design and platforms that may bring supramolecular sensors a step closer towards practical use for multiplexed or differential sensing, higher throughput operations, real-time monitoring, reporting of biological function, as well as for environmental studies.

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“…The system has been incorporated into a microfluidic device. 63 The assembly of lanthanides at interfaces has not been restricted to planar substrates. In an extension of their solution work (discussed above) Gunnlaugsson and coworkers have developed anion-sensing gold nanoparticles (AuNPs) utilising lanthanide luminescence to achieve transduction.…”

Section: Surface Assembled Lanthanide Architecturesmentioning

confidence: 99%

Exploiting lanthanide luminescence in supramolecular assemblies

et al. 2014

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Lanthanide ions, due to their unique photo-physical characteristics, have attracted considerable attention in recent years. Their long lifetimes, sharp, well-defined emission bands, and designable environmental sensitivity make them ideal for integration into switchable supramolecular assemblies where emission can report on local conformation and/or coupled energy levels (redox state). The immobilisation of lanthanide containing constructs on interfaces facilitates device integration, the fabrication of advanced sensory and molecular electronic platforms and presents a means by which conformational dynamics within molecular assemblies can be analysed. In this feature article we discuss the current and potential applications for lanthanide luminescence in supramolecular, switchable and surface bound architectures.

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A Supramolecular Sensing Platform for Phosphate Anions and an Anthrax Biomarker in a Microfluidic Device

Cited by 25 publications

References 70 publications

Rapid Detection of Bacillus anthracis Spores Using Immunomagnetic Separation and Amperometry

Rapid Detection of Bacillus anthracis Spores Using Immunomagnetic Separation and Amperometry

Evolution of Supramolecular Systems Towards Next-Generation Biosensors

Exploiting lanthanide luminescence in supramolecular assemblies

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