A Sunscreen-Tanning Compromise: 3D Visualization of the Actions of Titanium Dioxide Particles and Dihydroxyacetone on Human Epiderm

Puccetti, Germain; Leblanc, Roger M.

doi:10.1562/0031-8655(2000)0710426astcvo2.0.co2

Cited by 6 publications

(9 citation statements)

References 19 publications

(21 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Using melanin as an analogy, the time-dependent spectral shifts observed by us in our spectral imaging data and by others (13,15,19) might reveal information about the kinetics of DHA polymerization or binding to the skin (or both). As melanin aggregates, the absorption spectral intensity increases and becomes broader throughout the visible region (31).…”

Section: Spectral Shiftsupporting

confidence: 55%

“…Similarly, the emission also shifts to redder wavelengths as aggregation occurs (32). It is therefore reasonable to interpret the time-dependent spectral shifts observed for DHA on skin in both the absorption (19) and the emission (13,15, this work) as being evidence of the polymerization or binding of DHA to skin (or both) in its initial hours of contact with the skin. As the melanoidins are formed, either via aggregation or polymerization, and bound to the skin, the electronic structure changes simultaneously and thus affects the absorption and emission spectra (as well as the color).…”

Section: Spectral Shiftmentioning

confidence: 64%

“…The literature contains several reports about a time-dependent change in both the optical absorption (and tanning response) and fluorescence after DHA application on skin (13)(14)(15)19). For instance, Puccetti and Leblanc (19) reported that the absorption of light by cadaver skin increases at 270 nm (DHA absorption maximum) 3 h after DHA application. At later times (;10 h), there is an even larger absorption increase that broadens beyond 270 nm farther into the visible spectrum.…”

Section: Spectral Shiftmentioning

confidence: 99%

“…This color arises because of DHA's interactions with amino acids (15)(16)(17). Published data indicate that DHA penetrates through the stratum corneum, possibly reaching the top layers of the stratum granulosum (18,19), and binds to the skin forming a DHA-skin complex (DHA-SC). Because the DHA-SC is an integral part of the skin surface, its substantivity is only limited by the natural desquamation of the skin or by physical removal of the corneocytes (18,20).…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Fluorescence Spectral Imaging of Dihydroxyacetone on Skin In Vivo¶

Forest

Grothaus

Ertel

et al. 2003

Photochem Photobiol

View full text Add to dashboard Cite

Dihydroxyacetone (DHA) has been proposed as a potential alternative to dansyl chloride for use as a fluorescence marker on skin to assess stratum corneum turnover time in vivo. However, the fluorescence from DHA on skin has not been adequately studied. To address this void, a noninvasive, noncontact spectral imaging system is used to characterize the fluorescence spectrum of DHA on skin in vivo and to determine the optimal wavelengths over which to collect the DHA signal that minimizes the contributions from skin autofluorescence. The DHA-skin fluorescence signal dominates the 580-680 nm region of the visible spectrum when excited with ultraviolet radiation in the 320-400 nm wavelength region (UVA). An explanation of the time-dependent spectral features is proposed in terms of DHA polymerization and binding to skin.

show abstract

Section: Spectral Shiftsupporting

confidence: 55%

Section: Spectral Shiftmentioning

confidence: 64%

Section: Spectral Shiftmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Fluorescence Spectral Imaging of Dihydroxyacetone on Skin In Vivo¶

Forest

Grothaus

Ertel

et al. 2003

Photochem Photobiol

View full text Add to dashboard Cite

show abstract

“…This color arises because of DHA's interactions with amino acids (15–17). Published data indicate that DHA penetrates through the stratum corneum, possibly reaching the top layers of the stratum granulosum (18,19), and binds to the skin forming a DHA–skin complex (DHA–SC). Because the DHA–SC is an integral part of the skin surface, its substantivity is only limited by the natural desquamation of the skin or by physical removal of the corneocytes (18,20).…”

Section: Introductionmentioning

confidence: 99%

Fluorescence Spectral Imaging of Dihydroxyacetone on Skin In Vivo¶

Forest¹,

Grothaus²,

Ertel³

et al. 2007

Photochemistry and Photobiology

View full text Add to dashboard Cite

Dihydroxyacetone (DHA) has been proposed as a potential alternative to dansyl chloride for use as a fluorescence marker on skin to assess stratum corneum turnover time in vivo. However, the fluorescence from DHA on skin has not been adequately studied. To address this void, a noninvasive, noncontact spectral imaging system is used to characterize the fluorescence spectrum of DHA on skin in vivo and to determine the optimal wavelengths over which to collect the DHA signal that minimizes the contributions from skin autofluorescence. The DHA‐skin fluorescence signal dominates the 580–680 nm region of the visible spectrum when excited with ultraviolet radiation in the 320–400 nm wavelength region (UVA). An explanation of the time‐dependent spectral features is proposed in terms of DHA polymerization and binding to skin.

show abstract