A summary of potyvirus taxonomy and definitions

Barnett, O. W.

doi:10.1007/978-3-7091-6920-9_45

Cited by 26 publications

(14 citation statements)

References 23 publications

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“…For this virus family, in which members are defined on the basis of particle morphology, a positive sense ssRNA genome, and intercellular formation of characteristic cylindrical inclusion bodies, the family-genus-species concept has recently been proposed as the main system of classification [2,3]. Within Potyviridae, four genera are distinguished on the basis of their mode of transmission, i.e., the genus Potyvirus (aphid-borne), the genus Byrnovirus (fungal-borne), the genus Rymovirus (mite-borne), and the possible genus Ipomovirus (whitefly-borne) [2,3]. Of these four, the genus Potyvirus is the best characterized, though classical biological approaches to identify separate potyviruses and their strains, like host range, symptomatology and serology, have so far failed to supply reliable criteria for the distinction between all viruses within this group.…”

Section: Introductionmentioning

confidence: 99%

Taxonomic relationships between distinct potato virus Y isolates based on detailed comparisons of the viral coat proteins and 3′-nontranslated regions

Vlugt¹,

Goldbach²

1993

Archives of Virology

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Detailed comparisons were made of the sequences of the coat protein (CP) cistrons and 3'-nontranslated regions (3'-NTR) of 21 (geographically) distinct isolates of potato virus Y (PVY) and a virus isolate initially described as pepper mottle virus (PepMoV). Multiple sequence alignments and phylogenetic relationships based on these alignments resulted into a subgrouping of virus isolates which largely corresponded with the historical strain differentiation based on biological criteria as host range, symptomatology and serology. Virus isolates belonging to the same subgroup shared a number of characteristic CP amino acid and 3'-NTR nucleotide residues indicating that, by using sequences from the 3'-terminal region of the potyvirus genome, a distinction could be made between different isolates of one virus species as well as between different virus species. RNA secondary structure analysis of the 3'-NTR of twelve PVY isolates revealed four major stem-loop structures of which, surprisingly, the loop sequences gave a similar clustering of isolates as resulting from the overall comparisons of CP and 3'-NTR sequences. This implies a biological significance of these structural elements.

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Section: Introductionmentioning

confidence: 99%

Taxonomic relationships between distinct potato virus Y isolates based on detailed comparisons of the viral coat proteins and 3′-nontranslated regions

Vlugt¹,

Goldbach²

1993

Archives of Virology

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“…Moreover, molecular phylogeny based upon coat protein sequences has revealed subgroupings of potyviruses. One of these is the 'PVY subgroup' which contains PVY and pepper mottle virus (PepMoV) (Barnett, 1992).…”

Section: Introductionmentioning

confidence: 99%

“…Potato isolates have been separated into three main strains according to the symptoms they induce on potato and tobacco (De Bokx & Huttinga, 1981). The term 'strain' defines a collection of isolates causing the same symptoms (Barnett, 1992). The PVY N strain induces typical 'tobacco veinal necrosis' of Nicotiana tabacum whereas the PVY ° strain induces a non-necrotic mosaic on this host.…”

Section: Introductionmentioning

confidence: 99%

Sequence polymorphism in the 5'NTR and in the P1 coding region of potato virus Y genomic RNA

Tordo

Chachulska

Fakhfakh

et al. 1995

Journal of General Virology

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Potato virus Y (PVY) the type member of the genus Potyvirus, occurs wodd-wide as isolates which differ in host range and the type of symptoms caused. The sequences of a 5' segment of viral RNA overlapping the 5' non-translated region (5'NTR) alone (ten isolates) or the 5'NTR and the adjacent P1 coding region (eight isolates) were established. These data were used to quantify the polymorphism in the Y-terminal part of the PVY genome. Nucleotide sequence identity between isolates ranged from 66-100 % in the 5'NTR and from 70-100% in the P1 coding region. The lowest amino acid sequence similarity between PVY P1 was 77%, illustrating the high variability of this protein in the PVY species. Phylogenetic trees based on either 5'NTR or P1

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“…The virus is a member of the proposed genus Potyvirus (family Potyviridae) [1] and is spread in nature by aphids [4]. PPV particles are flexuous rods about 700 × 11 nm composed of a single stranded RNA molecule of almost 10000 nucleotides and a single coat protein (CP).…”

Section: Introductionmentioning

confidence: 99%

“…The potyvirus group presents complex serological relationships among members, which since immunological techniques are important tools for detection and identification of plant viruses, sometimes complicates the establishment of efficient strategies for virus diseases control based upon an appropriate diagnosis [1].…”

Section: Introductionmentioning

confidence: 99%

Production and characterization of monoclonal antibodies to plum pox virus and their use in differentiation of Mediterranean isolates

López‐Moya

Sanz²,

Cambra

et al. 1994

Archives of Virology

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Monoclonal antibodies (MAbs) specific to plum pox virus (PPV) were prepared by fusing myeloma cell lines to spleen cells of mice immunized with purified virus, including virus prepared with protease inhibitors to preserve the integrity of the coat protein (CP). The characterized MAbs could be used in ELISA to differentiate several Mediterranean PPV isolates differing in their geographical origin and CP size. At least seven antigenic sites could be established based on the recognition pattern and competition binding analysis, and the epitopes could be classified in three groups by Western blot analysis of intact and trypsin digested virus particles. By means of electron microscopy the epitopes could be seen to be located on the surface of the virus particles.

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A summary of potyvirus taxonomy and definitions

Cited by 26 publications

References 23 publications

Taxonomic relationships between distinct potato virus Y isolates based on detailed comparisons of the viral coat proteins and 3′-nontranslated regions

Taxonomic relationships between distinct potato virus Y isolates based on detailed comparisons of the viral coat proteins and 3′-nontranslated regions

Sequence polymorphism in the 5'NTR and in the P1 coding region of potato virus Y genomic RNA

Production and characterization of monoclonal antibodies to plum pox virus and their use in differentiation of Mediterranean isolates

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