A sucrose transporter, LjSUT4, is up-regulated during Lotus japonicus nodule development

Flemetakis, Emmanouil; Dimou, Maria; Cotzur, Daniela; Efrose, Rodica; Aivalakis, Georgios; Colebatch, Gillian; Udvardi, Michael K.; Katinakis, Panagiotis

doi:10.1093/jxb/erg179

Cited by 47 publications

(31 citation statements)

References 10 publications

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“…S13A). We also tested whether WT and mutant roots differed in expression levels of genes that have been reported to contribute to the metabolic state established between host and nitrogen-fixing symbiont (37)(38)(39)(40). We found comparable transcript levels of Nodulin26, Nodulin70, Sucrose transporter4, and Invertase1 in the tested genotypes, suggesting similar metabolic responses in the roots of the WT and mutants (SI Appendix, Fig.…”

Section: Resultsmentioning

confidence: 87%

Root nodule symbiosis in Lotus japonicus drives the establishment of distinctive rhizosphere, root, and nodule bacterial communities

Zgadzaj

Garrido‐Oter

Jensen

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

269

217

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Lotus japonicus has been used for decades as a model legume to study the establishment of binary symbiotic relationships with nitrogen-fixing rhizobia that trigger root nodule organogenesis for bacterial accommodation. Using community profiling of 16S rRNA gene amplicons, we reveal that in Lotus, distinctive noduleand root-inhabiting communities are established by parallel, rather than consecutive, selection of bacteria from the rhizosphere and root compartments. Comparative analyses of wild-type (WT) and symbiotic mutants in Nod factor receptor5 (nfr5), Nodule inception (nin) and Lotus histidine kinase1 (lhk1) genes identified a previously unsuspected role of the nodulation pathway in the establishment of different bacterial assemblages in the root and rhizosphere. We found that the loss of nitrogen-fixing symbiosis dramatically alters community structure in the latter two compartments, affecting at least 14 bacterial orders. The differential plant growth phenotypes seen between WT and the symbiotic mutants in nonsupplemented soil were retained under nitrogen-supplemented conditions that blocked the formation of functional nodules in WT, whereas the symbiosis-impaired mutants maintain an altered community structure in the nitrogen-supplemented soil. This finding provides strong evidence that the root-associated community shift in the symbiotic mutants is a direct consequence of the disabled symbiosis pathway rather than an indirect effect resulting from abolished symbiotic nitrogen fixation. Our findings imply a role of the legume host in selecting a broad taxonomic range of root-associated bacteria that, in addition to rhizobia, likely contribute to plant growth and ecological performance.Lotus japonicus | microbiota | symbiosis | 16S | nitrogen fixation

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Section: Resultsmentioning

confidence: 87%

Root nodule symbiosis in Lotus japonicus drives the establishment of distinctive rhizosphere, root, and nodule bacterial communities

Zgadzaj

Garrido‐Oter

Jensen

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

269

217

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“…Supply of carbon to infected cells may require symplastic transport from uninfected cells, since uninfected, but not infected, cells of Vicia faba are able to actively take up Glc or Suc from the apoplast (Peiter and Schubert, 2003). Consistent with this a Suc/H 1 cotransporter (LjSUT4) from L. japonicus nodules has been characterized and expression of this transporter in mature nodules was restricted mainly to the vascular bundles and nodule parenchymatous cells, while no hybridization signal could be detected in the cells of the central tissue (Flemetakis et al, 2003;Colebatch et al, 2004). Taken together with the expression of Suc synthase transcripts from soybean (Glycine max) nodules, this indicates that uninfected cells actively accumulate sugars and convert these to organic acids, which may then be released to the apoplast (Kavroulakis et al, 2000).…”

Section: Sucrose Transport and Carbon Provision To The Bacteroidmentioning

confidence: 80%

Nutrient Sharing between Symbionts

White

Prell²,

James³

et al. 2007

Plant Physiology

204

158

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In this review, we consider the exchange of nutrients between the host plant and the bacterial microsymbiont in nitrogen-fixing legume root nodules. During nodule formation, the host tissues and the bacterial microsymbiont develop in response to each other to form a specialized tissue that maintains an environment where nitrogen fixation can occur (Brewin, 2004;Mergaert et al., 2006;Prell and Poole, 2006). This complex development will not be considered here but, at the end of the process, specialized, nitrogen-fixing forms of the bacteria, known as bacteroids, reside in the plant cytosol, enclosed within plant-derived membranes. These organelle-like structures are known as symbiosomes; the plant-derived membrane that surrounds the bacteroid is the symbiosome (or peribacteroid) membrane and the space between the two is the symbiosome (or peribacteroid) space. An infected plant cell may be packed with thousands of symbiosomes. The exchange of nutrients that is fundamental to N 2 fixation therefore involves metabolism in the plant to provide carbon and nitrogen compounds to the bacteroids and to assimilate the metabolites that bacteroids release. Nutrients transferred between the symbionts must traverse both the symbiosome and the bacteroid membranes. It is clear that there is more than one pattern whereby successful nutrient exchange can take place: There are two basic types of legume nodules, determinate and indeterminate, and there are fundamental differences between the two in how they develop and in their carbon and nitrogen metabolism. This review focuses on the metabolism of carbon and nitrogen compounds in the symbionts and on the exchange of nutrients across the bacteroid and symbiosome membranes. Particular attention is paid to the movement of primary carbon and nitrogen sources and how they are utilized by both bacteroids and the plant.

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“…LjSUT4 is a Suc transporter in L. japonicus that is up-regulated during nodule development and presumably plays a role in Suc uptake into nodule cells (Flemetakis et al, 2003). Three members of the Suc/proton symporter subfamily (TC 2.A.2.4) of the Glycoside-Pentoside-Hexuronide:Cation Symporter family (TC 2.A.2) were identified in our analysis of Medicago transporters, but none of them was induced during nodulation.…”

Section: Transporters Involved In N 2 Fixation Symbiosismentioning

confidence: 88%

Genomic Inventory and Transcriptional Analysis of Medicago truncatula Transporters

Benedito

Dai

et al. 2009

Plant Physiology

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Transporters move hydrophilic substrates across hydrophobic biological membranes and play key roles in plant nutrition, metabolism, and signaling and, consequently, in plant growth, development, and responses to the environment. To initiate and support systematic characterization of transporters in the model legume Medicago truncatula, we identified 3,830 transporters and classified 2,673 of these into 113 families and 146 subfamilies. Analysis of gene expression data for 2,611 of these transporters identified 129 that are expressed in an organ-specific manner, including 50 that are nodule specific and 36 specific to mycorrhizal roots. Further analysis uncovered 196 transporters that are induced at least 5-fold during nodule development and 44 in roots during arbuscular mycorrhizal symbiosis. Among the nodule-and mycorrhiza-induced transporter genes are many candidates for known transport activities in these beneficial symbioses. The data presented here are a unique resource for the selection and functional characterization of legume transporters.

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A sucrose transporter, LjSUT4, is up-regulated during Lotus japonicus nodule development

Cited by 47 publications

References 10 publications

Root nodule symbiosis in Lotus japonicus drives the establishment of distinctive rhizosphere, root, and nodule bacterial communities

Root nodule symbiosis in Lotus japonicus drives the establishment of distinctive rhizosphere, root, and nodule bacterial communities

Nutrient Sharing between Symbionts

Genomic Inventory and Transcriptional Analysis of Medicago truncatula Transporters

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