A successful protocol for achieving anhydrobiosis of Gallus Gallus Domesticus spermatozoa while maintaining their fertility IN VIVO

Stanishevskaya, Olga I.; Silyukova, Yulia; Pleshanov, Nikolay; Kurochkin, Anton; Федорова, Е.С.; Radaev, Anton

doi:10.1016/j.cryobiol.2021.11.002

Cited by 2 publications

(1 citation statement)

References 23 publications

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“…Since 2000, sperm cells have been studied in 20 different mammalian species, including humans (Table 1), as ideal candidates for dehydration because of their low water content and compacted DNA. Inspired by early observations on fowl sperm dehydration during vitrification (Polge et al, 1949), a recent report on rooster sperm lyophilization represents very few efforts in bird sperm drying (Stanishevskaya et al, 2022). However, very few studies on partial or full dehydration have been conducted in oocytes and gonadal tissues (Table 1).…”

Section: Introductionmentioning

confidence: 99%

Damages and stress responses in sperm cells and other germplasms during dehydration and storage at nonfreezing temperatures for fertility preservation

Comizzoli

Amelkina

Lee

2022

Molecular Reproduction Devel

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Long‐term preservation of sperm, oocytes, and gonadal tissues at ambient temperatures has the potential to lower the costs and simplify biobanking in human reproductive medicine, as well as for the management of animal populations. Over the past decades, different dehydration protocols and long‐term storage solutions at nonfreezing temperatures have been explored, mainly for mammalian sperm cells. Oocytes and gonadal tissues are more challenging to dehydrate so little to no progress have been made. Currently, the detrimental effects of the drying process itself are better characterized than the impact of long‐term storage at nonfreezing temperatures. While structural and functional properties of germ cells can be preserved after dehydration, a long list of damages and stresses in nuclei, organelles, and cytoplasmic membranes have been reported and sometimes mitigated. Characterizing those damages and better understanding the response of germ cells and tissues to the stress of dehydration is fundamental. It will contribute to the development of optimal protocols while proving the safety of alternative storage options for fertility preservation. The objective of this review is to (1) document the types of damages and stress responses, as well as their mitigation in cells dried with different techniques, and (2) propose new research directions.

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Section: Introductionmentioning

confidence: 99%

Damages and stress responses in sperm cells and other germplasms during dehydration and storage at nonfreezing temperatures for fertility preservation

Comizzoli

Amelkina

Lee

2022

Molecular Reproduction Devel

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The effect of the mitochondrial-directed antioxidant Mito-TEMPO on the quality of rooster sperm in the cryopreservation and lyophilization cycle

Kurochkin,

Stanishevskaya,

Silyukova

et al. 2023

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In reproductive technologies applied in animal husbandry, using lyophilized semen is only possible with ICSI technology, due to complete loss sperm motility. However, the anatomical structure’s peculiarities and development of avian oocyte not allows use this technology. Strategies aimed at preserving kinetic apparatus of lyophilized sperm are the most relevant for birds. Increasing mitochondrial activity of cells due to its connection with energy metabolism and sperm motility may be one of the ways to solve this problem. However, similar studies on freeze-dried bird semen haven’t been conducted on this moment. The present study assessed effect of mitochondria-targeted antioxidant Mito-TEMPO on viability, mitochondria’s membrane potential and generation of intracellular hydrogen peroxide by rooster spermatozoa in a cryopreservation and lyophilization cycle. Proportion of cells with intact plasma membrane in lyophilized semen was higher using extenders containing an antioxidant. Addition of Mito-TEMPO at concentration of 40 µM to diluents T-20 and T-30 for the purpose of cryopreservation of semen with subsequent freezedrying allowed to increase proportion of cells with intact plasma membrane in lyophilized semen in comparison to control diluents (6.12 ± 0.11 % versus 10.19±0.46% and 13.19±1.30% versus 15.23±1.13%, respectively, p˂0.05). Effective concentration of Mito-TEMPO in diluents intended only for semen cryopreservation was significantly lower - 5 µM. Proportion of cells with intact plasma membrane was 47,97±0,64% versus 55,85±1,69% and 47,25±2,97% versus 63,21±1,78%, respectively, p˂0, 05. Dose-dependent effect of trehalose in cryoprotective diluents has been proven.

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A successful protocol for achieving anhydrobiosis of Gallus Gallus Domesticus spermatozoa while maintaining their fertility IN VIVO

Cited by 2 publications

References 23 publications

Damages and stress responses in sperm cells and other germplasms during dehydration and storage at nonfreezing temperatures for fertility preservation

Damages and stress responses in sperm cells and other germplasms during dehydration and storage at nonfreezing temperatures for fertility preservation

The effect of the mitochondrial-directed antioxidant Mito-TEMPO on the quality of rooster sperm in the cryopreservation and lyophilization cycle

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