A Suberized Exodermis is Required for Tomato Drought Tolerance

Cantó-Pastor, Alex; Kajala, Kaisa; Shaar-Moshe, Lidor; Manzano, Concepción; Timilsena, Prakash Raj; Bellis, Damien De; Gray, Sharon B.; Holbein, Julia; Yang, He; Mohammad, Sana; Nirmal, Niba; Suresh, Kiran; Ursache, Robertas; Mason, G. Alex; Gouran, Mona; West, Donnelly A.; Borowsky, Alexander T.; Shackel, Kenneth A.; Sinha, Neelima; Bailey‐Serres, Julia; Geldner, Niko; Li, Song; Franke, Rochus; Brady, Siobhán M.

doi:10.2139/ssrn.4272134

Cited by 5 publications

(6 citation statements)

References 66 publications

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“…7B). Members of the above-mentioned TF families have been reported to regulate other specialized metabolism pathways, such as anthocyanin 30 , cuticle 44 , suberin 45 , and glucosinolate 46 in other species. We speculate that yet unidentified homeodomain, ERF, and MYB TFs may contribute to the cell type specific expression of MIA biosynthetic genes in epidermis.…”

Section: Discussionmentioning

confidence: 99%

Cell-type aware regulatory landscapes governing monoterpene indole alkaloid biosynthesis in the medicinal plantCatharanthus roseus

Li,

Colinas,

Wood

et al. 2024

Preprint

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In plants, the biosynthetic pathways of some specialized metabolites are partitioned into specialized or rare cell types, as exemplified by the monoterpenoid indole alkaloid (MIA) pathway of Catharanthus roseus (Madagascar Periwinkle), the source of the anti-cancer compounds vinblastine and vincristine. In the leaf, the C. roseus MIA biosynthetic pathway is partitioned into three cell types with the final known steps of the pathway expressed in the rare cell type termed idioblast. How cell-type specificity of MIA biosynthesis is achieved is poorly understood. Here, we generated single-cell multi-omics data from C. roseus leaves. Integrating gene expression and chromatin accessibility profiles across single cells, as well as transcription factor (TF) binding site profiles, we constructed a cell-type-aware gene regulatory network for MIA biosynthesis. We showcased cell-type-specific transcription factors as well as cell-type-specific cis-regulatory elements. Using motif enrichment analysis, co-expression across cell types, and functional validation approaches, we discovered a novel idioblast specific TF (Idioblast MYB1, CrIDM1) that activates expression of late stage vinca alkaloid biosynthetic genes in the idioblast. These analyses not only led to the discovery of the first documented cell-type-specific TF that regulates the expression of two idioblast specific biosynthetic genes within an idioblast metabolic regulon, but also provides insights into cell-type-specific metabolic regulation.

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Section: Discussionmentioning

confidence: 99%

Cell-type aware regulatory landscapes governing monoterpene indole alkaloid biosynthesis in the medicinal plantCatharanthus roseus

Li,

Colinas,

Wood

et al. 2024

Preprint

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“…To determine the potential molecular mechanisms that underly these changes, we conducted transcriptome profiling of the sorghum root systems at both two and three weeks post-infection and in the presence and absence of Striga. Indeed, the transcription of several sorghum orthologs of suberin biosynthetic genes or putative transporters -Sobic.003G368100 (SbASFTa) and Sobic.005G122800 (SbASFTb) (Gou et al, 2009;Molina et al, 2009), Sobic.004G010300 (SbGPAT4/8) (Canto-Pastor et al, 2022), Sobic.009G16200 (SbGPAT5/7) (Beisson et al, 2007), Sobic.001G413700 (SbABCG1/SbABCG2/ABCG6/ABCG20a, Supplementary Fig. 3) (Yadav et al, 2014) were upregulated in "natural" soil compared to "sterilized" soil in the presence and absence of Striga (Fig.…”

Section: The Soil Microbiome Modifies Root Cellular Anatomy and Corre...mentioning

confidence: 99%

“…In the first stage, there is an absence of suberin within the root meristem, followed by a "patchy" zone and a fully suberized zone in differentiated root (Kajala et al, 2021;Canto-Pastor et al, 2022). Typically, suberin in roots is quantified by measuring its level in few representative cells from the cross section (quantitative suberization), or by measuring the proportion of the non-suberized, "patchy" and fully suberized zone within the root length (developmental suberization) (Kajala et al, 2021;Canto-Pastor et al, 2022). The latter is challenging for sorghum, due to the high level of autofluorescence signal from its roots interfering with the suberin signal from fluorol yellow stain.…”

Section: Specific Bacterial Isolates Prevent Haustoria Formation and ...mentioning

confidence: 99%

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The Soil Microbiome Reduces Striga Infection of Sorghum by Modulation of Host-Derived Signaling Molecules and Root Development

Kawa

Thiombiano

Shimels

et al. 2023

Preprint

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“…The endodermis is characterized by localized lignin depositions known as "Casparian strips" and suberin lamellae, which accumulate across the entire plasma membrane (Doblas et al ., 2017; Naseer et al ., 2012). Similarly, the exodermis is a ligno-suberized layer in which lignin deposition occurs in a U-shaped fashion before suberin accumulation (Cantó-Pastor et al ., 2024; Manzano et al ., 2022). The cork cell wall is also impregnated with both polymers (Kosma et al ., 2015; Serra et al ., 2022; Wunderling et al ., 2018).…”

Section: Introductionmentioning

confidence: 99%

MYB68 orchestrates cork differentiation by regulating stem cell proliferation and suberin deposition

Molina,

Horvath,

Zhang

et al. 2024

Preprint

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Plants have developed specialized barriers to protect and isolate the inner tissues from the environment while maintaining homeostasis. Different barriers are present in various organs and at different growth stages. During secondary growth, the periderm acts as the protective tissue, covering roots, stems, and branches as they become thick. The periderm is a dynamic barrier comprising a stem cell niche known as the cork cambium, which bifacially divides to generate the phelloderm inward and the cork outward. Cork cells have a unique cell wall impregnated with suberin and lignin polymers, essential for the barrier function. Despite its importance, the differentiation process that forms new cork cells from the stem cell is largely unknown. In this work, we identify members of the MYB36-subclade transcription factors as key regulators of cork differentiation. On the one hand, this set of transcription factors promotes suberin deposition by inducing the expression of enzymes involved in all steps of suberin biosynthesis, including the recently discovered suberin-polymerizing enzymes GDS Lipases; on the other hand, it represses cork cambium proliferation. Furthermore, we demonstrated that suberin deposition in the cork is a robust process regulated by a complex network of transcription factors, including other MYB transcription factors that activate suberin deposition in the endodermis. However, only members of the MYB36 subclade can repress cell proliferation in different developmental contexts, highlighting general and specific functions for MYB transcription factors. These findings have broad applicability, as tissue-specific manipulation of MYB activity has the potential for improving traits of biotechnological interest, such as thicker periderms and more suberized cork layers, and for assessing how these traits affect plant performance in response to stresses.

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A Suberized Exodermis is Required for Tomato Drought Tolerance

Cited by 5 publications

References 66 publications

Cell-type aware regulatory landscapes governing monoterpene indole alkaloid biosynthesis in the medicinal plantCatharanthus roseus

Cell-type aware regulatory landscapes governing monoterpene indole alkaloid biosynthesis in the medicinal plantCatharanthus roseus

The Soil Microbiome Reduces Striga Infection of Sorghum by Modulation of Host-Derived Signaling Molecules and Root Development

MYB68 orchestrates cork differentiation by regulating stem cell proliferation and suberin deposition

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