A subacute presentation ofMycoplasma hominisprosthetic valve endocarditis

Abstract

Mycoplasma hominisis a rare but important cause of prosthetic valve endocarditis. It is usually associated with acute progression of symptoms and can be difficult to diagnose as it does not grow in standard culture media. We report a case of an immunocompetent man in his 70s who presented with 14-month subacute decline with shortness of breath and evidence of a splenic infarct. Following a redo aortic valve replacement and diagnosis ofM. hoministhrough 16S ribosomal ribonucleic acid PCR, he improved clinically with oral doxycycline therapy. He remained well at follow-up 2 years post-cessation of antibiotics. We present a literature review highlighting the role of PCR testing in the microbiological identification ofM. hominis.

Optimization and standardization of mNGS-based procedures for the diagnosis of Mycoplasma periprosthetic joint infection: A novel diagnostic strategy for rare bacterial periprosthetic joint infection

“…16S PCR is the earliest and most widely used in PJI pathogenic microorganism isolation, which could simply and quickly amplify the genes or fragments by specific or non-specific primers, and the amplified products could be sequenced, then the species of pathogenic bacteria could be obtained (Saglani et al, 2005;Fida et al, 2021). This was an effective tool for detecting Mycoplasma and widely used in recent years (Jensen et al, 2003;McAuliffe et al, 2005;Kim et al, 2022). However, this method could not effectively detect true pathogenic bacteria when there was mixed infection, and it could not distinguish contaminated bacteria from true pathogenic bacteria.…”