A stylar ribonuclease assay to detect self-compatible seedlings in almond progenies

Bošković, R.; Tobutt, K. R.; Duval, Henri; Batlle, I.; Dicenta, F.; Vargas, F.J.

doi:10.1007/s001220051299

Cited by 64 publications

(71 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Self-compatible variants appear when this activity disappears or strongly decreases. This is the case in Prunus dulcis (Dicenta and Garcia 1993;Socias i Compani et al 1995;Bošković et al 1999), Lycopersicon peruvianum (Kowyama et al 1994), Petunia inflata (Huang et al 1994) and Pyrus pyrifolia (Sassa et al 1997). Although little is known about the biochemical basis of self-incompatibility in Coffea, one could reasonably argue that self-compatibility in C. heterocalyx could comply with such a model.…”

Section: Model Of Self-compatibility Inheritancementioning

confidence: 96%

Introgression of self-compatibility from Coffea heterocalyx to the cultivated species Coffea canephora

et al. 2002

View full text Add to dashboard Cite

Self-compatibility segregation was assessed in two successive backcross progenies originating from an interspecific cross between Coffea canephora (self-incompatible) and Coffea heterocalyx (self-compatible). After self- and cross-pollination, pollen tube behaviour in styles was observed under ultraviolet fluorescence microscopy and fruit-set was determined at harvesting time. Segregation ratios in the two progenies were consistent with monofactorial control of self-compatibility. Self-compatible plants exhibited higher fruit-set than self-incompatible ones in open-pollination conditions. Segregation of AFLP markers was scored in the first backcross progeny. By molecular linkage analysis, the S locus could be mapped to a short linkage group.

show abstract

Section: Model Of Self-compatibility Inheritancementioning

confidence: 96%

Introgression of self-compatibility from Coffea heterocalyx to the cultivated species Coffea canephora

et al. 2002

View full text Add to dashboard Cite

show abstract

“…Stylar proteins were extracted following the method of Boskovic et al (1997), and the stylar ribonucleases were subsequently assayed as described by Boskovic et al (1999). Cultivars with confirmed S alleles were included for comparison.…”

Section: Stylar Ribonuclease Analysismentioning

confidence: 99%

“…The S 5 allele, assigned to Desmayo Largueta (S 1 S 5 ) and Gabaix (S 5 S 10 ) by Boskovic et al (1997Boskovic et al ( , 2003, was observed to be different to that reported for Texas (S 1 S 5 ). Furthermore, the S 10 allele assigned to Ardechoise (S 1 S 10 ) and Ferrastar (S 2 S 10 ) by Boskovic et al (1999) and to Achaak (S 2 S 10 ) by Ortega (2002) migrated to the same position as the S 5 from Desmayo Largueta but to a different position to that of S 10 of Gabaix. In other fruit species, such as apple (Van Nerum et al 2001;Broothaerts 2003) and cherry (Wiersma et al 2001;Sonneveld et al 2003), genotype discrepancies have also been found in the identification of S alleles by ribonuclease analysis.…”

Section: Introductionmentioning

confidence: 96%

“…Other S alleles were detected using zymograms to determine the presence of particular stylar ribonucleases Boskovic et al 1997Boskovic et al , 1998Boskovic et al , 1999Boskovic et al , 2003Duval et al 1998;Mnejja et al 2002;Ortega and Dicenta 2003). Sixteen incompatibility groups involving 24 S alleles (S 1 -S 23 and S 7A ) are reported in almond (groups I-XV and group O of cultivars with unique genotypes) (Kester and Gradziel 1996;Boskovic et al 2003).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Self-incompatibility genotypes in almond re-evaluated by PCR, stylar ribonucleases, sequencing analysis and controlled pollinations

López¹,

Mnejja²,

Rovira³

et al. 2004

Theor Appl Genet

View full text Add to dashboard Cite

As part of the almond breeding programme at IRTA, we investigated the S genotypes of several cultivars using a combination of RNase zymograms, testcrosses, pollen-tube growth analysis and molecular identification by PCR analysis. For some of the cultivars examined, discrepancies appeared between their S alleles as reported in the literature and those found in this investigation, leading to a re-evaluation of their S genotypes. Analysis of the stylar ribonucleases (RNases), which are known to correlate with S alleles, of cvs. Achaak, Ardechoise, Desmayo Largueta, Ferrastar, Gabaix, Garbi, Glorieta, Languedoc, Primorskiy and Texas revealed inconsistencies with respect to the S5 and S10 alleles. However, PCR with the conserved primer pair AS1II/AmyC5R failed to detect any of these inconsistencies. When the S alleles from Desmayo Largueta, Gabaix, Primorskiy and Texas were sequenced, Texas and Primorskiy were found to carry the reported S5 allele, while Desmayo Largueta and Gabaix carried a new allele, which has been tentatively denoted as S25 This new S allele, previously reported to be S10, was also identified in Achaak, Ardechoise and Ferrastar. The proposed new S genotypes are Achaak (S2S25), Ardechoise (S1S25), Desmayo Largueta (S1S25), Ferrastar (S2S25) and Gabaix (S10S25). The S alleles of Garbi, Glorieta, Languedoc, Texas and Primorskiy remain as reported in the literature. Testcrosses in the field and laboratory confirmed the new S genotypes. One cultivar (Gabaix) could be assigned to the existing cross-incompatibility group O of unique genotypes, and two new groups were established (XVI and XVII) consisting of two cultivars each. The clarification of these S alleles will be useful in almond breeding programmes and for planning new commercial orchards in the future.

show abstract

“…Self-compatible almonds carry the S f -RNase allele, which does not code for ribonuclease activity. Hence, a co-dominant stylar ribonuclease assay was developed, where genotypes having only one S-RNase isoenzyme were assumed to be heterozygotes (S f S i ; S f : allele for self-compatibility, S i : allele for selfincompatibility), while those having no active S-RNases were homozygous, selfcompatible (S f S f ) accessions (Boskovic et al, 1999). Later, alleles with coding and 5′ regulatory sequences identical to S f were also isolated from selfincompatible cultivars, showing that the different expression of S f is independent of the complete genetic identity found in the whole chromosome region bordering the S-locus (Fernández i Marti et al, 2010).…”

mentioning

confidence: 99%

Review of genetic diversity studies in almond (Prunus dulcis)

Szikriszt

Hegedűs

Halász

2011

Acta Agronomica Hungarica

View full text Add to dashboard Cite

Almond [Prunus dulcis (Mill.) D.A. Webb.] is cultivated for its nuts and is one of the oldest domesticated plants. Due to the genetically controlled self-incompatibility system that operates in this species, almond is characterized by great genetic diversity, the estimation of which has benefited from a range of marker techniques, including morphological characteristics, isoenzyme detection and molecular markers. Among the DNA-based molecular markers, simple sequence repeats (SSR) have been used most widely, although analyses have ranged from restriction fragment length polymorphism to the most recent single nucleotide polymorphism detection methods. Molecular markers have also been used to trace specific agronomic traits, e.g. self-(in)compatibility or kernel bitterness. Genetic diversity studies in almond have not revealed a direct relationship between the level of diversity and the origin of the germplasm. This might be explained by the relatively recent occurrence of self-compatibility in almond, which has not yet caused a serious loss of genetic diversity. The markers reviewed will be useful in monitoring and maintaining genetic diversity in almond breeding programmes, while others may permit marker-assisted selection for favourable agronomic traits. The cultivation, breeding and conservation of wild-growing almonds may equally benefit from the genetic diversity studies (especially those applying molecular markers).

show abstract

A stylar ribonuclease assay to detect self-compatible seedlings in almond progenies

Cited by 64 publications

References 9 publications

Introgression of self-compatibility from Coffea heterocalyx to the cultivated species Coffea canephora

Introgression of self-compatibility from Coffea heterocalyx to the cultivated species Coffea canephora

Self-incompatibility genotypes in almond re-evaluated by PCR, stylar ribonucleases, sequencing analysis and controlled pollinations

Review of genetic diversity studies in almond (Prunus dulcis)

Contact Info

Product

Resources

About