A study to detect <i>Helicobacter pylori</i> in fresh and archival specimens from patients with interstitial cystitis, using amplification methods

Agarwal, Meena; Dixon, Ronald

doi:10.1046/j.1464-410x.2003.04221.x

Cited by 13 publications

(7 citation statements)

References 9 publications

(14 reference statements)

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“…Organisms that may be important clinically but are difficult or impossible to culture have been research targets for the application of PCR technology. Amplification of DNA from pathogens by PCR is rapidly becoming established as a method of locating very low quantities of an infective agent present in the clinical samples 52 …”

Section: Discussionmentioning

confidence: 99%

Deoxyribonucleic acid‐based diagnostic techniques to detect Helicobacter pylori

Ruzsovics

Molnár

Tulassay

2004

Aliment Pharmacol Ther

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SUMMARYHelicobacter pylori is an important cause of many gastrointestinal disorders, ranging from chronic gastritis to gastric lymphoma and adenocarcinoma.The deoxyribonucleic acid-based assays have the potential to be a powerful diagnostic tool given its ability to specifically identify H. pylori deoxyribonucleic acid. Markers used to include general H. pylori structures and pathogenetic factors like ureaseA, cagA, vacA, iceA. Deoxyribonucleic acid or bacterial ribonucleic acid for polymerase chain reaction assays can be collected from gastric biopsy, gastric juice, stool, buccal specimens. Polymerase chain reaction can yield quantitative and genotyping results with sensitivity and specificity that approaches 100%. A clear trend in the direction of the determination of quantitative H. pylori infection by real-time polymerase chain reaction can be observed. Fluorescent in situ hybridization is suggested for routine antibiotic resistance determination. To identify the organism, deoxyribonucleic acid structure and its virulence factors may be feasible by using oligonucleotide microarray specifically recognizing and discriminating bacterial deoxyribonucleic acid and various virulence factors. Deoxyribonucleic acid-based H. pylori diagnosis yields higher sensitivity, however, specificity requires sophisticated labour environment and associated with higher costs.

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Section: Discussionmentioning

confidence: 99%

Deoxyribonucleic acid‐based diagnostic techniques to detect Helicobacter pylori

Ruzsovics

Molnár

Tulassay

2004

Aliment Pharmacol Ther

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“…The lower urinary tract and bladder are not sterile, as has been traditionally assumed, but contain a resident microbiota, including organism’s refractory to standard culturing techniques [12,14,15]. There have been some attempts to employ earlier generation molecular diagnostic techniques to search for the presence of causative organisms in patients with negative urine cultures and a diagnosis of IC/BPS [5,6,7,8,9,10]. However, findings have been inconclusive [19].…”

Section: Discussionmentioning

confidence: 99%

“…Although active urinary tract infection excludes the diagnosis, and empiric antibiotic therapy is typically unhelpful, a bacterial etiology has never been excluded as a mechanism in IC/BPS, and such an association has been suggested in a small but perhaps significant number of IC/BPS patients [5,6,7,8,9,10]. The microbiologic diagnosis of infection in the bladder has traditionally been based on cultivation techniques in which bacteria are grown from voided urine spread on culture plates, which does not have the nutritive and environmental conditions required to support the growth of many microorganisms.…”

Section: Introductionmentioning

confidence: 99%

A Culture-Independent Analysis of the Microbiota of Female Interstitial Cystitis/Bladder Pain Syndrome Participants in the MAPP Research Network

Nickel

Stephens‐Shields

Landis

et al. 2019

JCM

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We surveyed urine microbiota of females diagnosed with interstitial cystitis/bladder pain syndrome (IC/BPS) and matched control participants enrolled in the National Institutes of Health (NIH) Multidisciplinary Approach to the Study of Chronic Pelvic Pain (MAPP) Research Network using the culture-independent methodology. Midstream urine specimens were analyzed with the Plex-ID molecular diagnostic platform that utilizes polymerase chain reaction–electrospray ionization–time-of-flight–mass spectrometry (PCR-ESI-TOF MS) to provide a comprehensive identification of bacterial and select fungal species. IC/BPS and control participants were evaluated for differences (presence, diversity, and abundance) in species and genus. Urine specimens obtained from 181 female IC/BPS and 182 female control participants detected a total of 92 species (41 genera). Mean (SD) species count was 2.49 (1.48) and 2.30 (1.28) among IC/BPS and control participants, respectively. Overall species composition did not significantly differ between IC/BPS and control participants at any level (p = 0.726 species level, p = 0.222 genus level). IC/BPS participants urine trended to an overabundance of Lactobacillus gasseri (p = 0.09) detected but had a lower prevalence of Corynebacterium compared with control participants (p = 0.002). The relative abundance data analysis mirrored the prevalence data differences with no significant differences in most species or genus abundance other than Lactobacillus gasseri and Corynebacterium (p = 0.08 and p = 0.001, respectively). No cause and/or effect conclusion can be drawn from this observation, but it suggests that a more comprehensive evaluation (vaginal, bowel, catheterized bladder and/or tissue-based specimens) of the lower urinary tract microbiota in IC/BPS patients is warranted.

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“…More series or single cases of extragastric H. pylori ‐related diseases have been published in various fields [91–95].…”

Section: Miscellaneousmentioning

confidence: 99%

Helicobacter pylori and Extragastric Diseases – Other Helicobacters

et al. 2004

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Reports on Helicobacter pylori and extragastric diseases have almost doubled this year compared with last year, bearing witness to the persistent scientific interest in this branch of Helicobacter-related pathology. Data belong increasingly to the area of vascular medicine, as well as hematology, dermatology, pediatrics and other fields. Unfortunately, these studies show overall controversial results, due to the impact of several confounding factors, and to the difficulty of recruiting homogeneous patient populations. Furthermore, many studies continue to be conducted on Helicobacter species other than H. pylori, focusing on animal models of gastroenterological illnesses which may retain strong similarities with human diseases. In this paper, taxonomy, detection and characterisation of Helicobacter spp. will be reviewed, together with the most important data issued this year on other Helicobacters and animal models.

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A study to detect Helicobacter pylori in fresh and archival specimens from patients with interstitial cystitis, using amplification methods

Abstract: As there was no H. pylori DNA in any of the samples from patients with IC, it is an unlikely candidate in the pathogenesis of IC.

Cited by 13 publications

References 9 publications

Deoxyribonucleic acid‐based diagnostic techniques to detect Helicobacter pylori

Deoxyribonucleic acid‐based diagnostic techniques to detect Helicobacter pylori

A Culture-Independent Analysis of the Microbiota of Female Interstitial Cystitis/Bladder Pain Syndrome Participants in the MAPP Research Network

Helicobacter pylori and Extragastric Diseases – Other Helicobacters

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