A study on the utility of immobilized cells of indigenous bacteria for biodegradation of reactive azo dyes

Pandey, Koushik; Saha, Purbasha; Rao, Kokati Venkata Bhaskara

doi:10.1080/10826068.2019.1692219

Cited by 23 publications

(6 citation statements)

References 53 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Two indigenous isolated strains were used for decolorization studies. Upon immobilization with Ca/Alg gel beads, dye removal occurred faster and peaked at 97.5% and 988.2% within 48 h (Pandey et al, 2020). In another work, Swarnkumar Reddy experimented on removing Reactive Red 120 using immobilized and free cells of Pseudomonas guariconensis in a Ca/Alg biocarrier matrix.…”

Section: Resultsmentioning

confidence: 99%

Improved application of immobilized Enterobacter cloacae into a bio‐based polymer for Reactive Blue 19 removal, an eco‐friendly advancement in potential decolorizing systems

Nazari,

Jookar Kashi,

Nazari

2024

Water Environment Research

View full text Add to dashboard Cite

The widespread use of highly complex synthetic dyes like reactive dyes in the textile industry has some adverse environmental impacts and deserves close attention. Biological treatment of these effluents utilizing various species of bacteria with remarkable efficiency in dye removal is still considered promising. Our current study deals with immobilizing an isolated bacterial strain into calcium alginate (Ca/Alg) gel beads and using it to treat pernicious pollutants like synthetic dyes. A potential Reactive Blue 19 (RB19)‐degrading Enterobacter cloacae strain A1 was isolated from the Kashan textile industry and was characterized by 16S rDNA gene sequencing. The decolorization ability of strain A1 was assessed by time‐based studies using free bacterial cells/immobilized in Ca/Alg. Based on the results of the 16S rDNA gene sequencing, it appears that strain A1 belonged to E. cloacae, with a 99.74% similarity. The findings suggest that immobilized strain A1 accomplished maximum decolorization activity compared with the free cells. The immobilized strain could utterly decompose and decolorize 0.05 mg/mL of RB19 within 48 h, while the free bacterial strain decolorized RB19 within 5 days. Moreover, Ca/Alg gel beads can maintain their efficiency for over three decolorization cycles. Further infrared spectroscopy (FTIR) and gas chromatograph mass spectrometer (GC/MS) investigation declared complete RB19 decomposition on reaction products. Artemia salina was used to investigate the toxicity of dye and its degraded metabolites. The LC50 values signified the pure dye as very toxic with 0.01 mg/mL concentration, while after‐treatment products showed no toxic effect on larvae. This immobilization technique increased the applicability of bacterial strain for dye removal. It was beneficial for the decolorization of RB19 from textile wastewater due to a remarkable reduction in time. Notably, strain A1‐immobilized beads can maintain their activity for three consecutive decolorization cycles without a considerable decrease in efficiency.Practitioner Points The remarkable capacity of immobilized Enterobacter cloacae strain A1 for Reactive Blue 19 (RB19) removal Immobilized A1 strain showed two‐fold higher removal than free one over 48 h A promising method for enhancing RB19 decolorization Decolorization was due to degradation based on UV–Vis, FTIR, and GC/MS analysis Non‐toxic posttreatment products for Artemia

show abstract

Section: Resultsmentioning

confidence: 99%

Improved application of immobilized Enterobacter cloacae into a bio‐based polymer for Reactive Blue 19 removal, an eco‐friendly advancement in potential decolorizing systems

Nazari,

Jookar Kashi,

Nazari

2024

Water Environment Research

View full text Add to dashboard Cite

show abstract

“…and Lysinibacillus sp. against two azo dyes i.e., Reactive Orange 16 and Reactive Blue 250 was reported [ 14 ]. Free cells of Bacillus .…”

Section: Resultsmentioning

confidence: 99%

“…Furthermore, cell immobilization was used to study the rate of dye decolorization in comparison with freely suspended bacterial cells. Cell immobilization was found to protect the cells from the lethal effects of dyes and their by-products, maintain high biomass concentration, was cost-effective and re-usable [ 14 ]. The biodegradation kinetics of the azo dyes with respect to the degradation potential of microbial communities has not been studied in detail, yet can provide a deeper understanding of the specific biodegradation mechanisms.…”

Section: Introductionmentioning

confidence: 99%

Decolorization of azo dyes by a novel aerobic bacterial strain Bacillus cereus strain ROC

et al. 2022

View full text Add to dashboard Cite

Synthetic dyes are widely used as colorant compounds in various industries for different purposes. Among all the dyestuffs, azo dyes constitute the largest and the most used class of dyes. These dyes and their intermediate products are common contaminants of ground water and soil in developing countries. Biological methods have been found to be promising for the treatment and degradation of these compounds. In the present study, we focused on the biological removal of azo dyes (Reactive orange 16 and Reactive black 5) under aerobic conditions using an indigenous bacterial strain isolated from contaminated industrial areas. The bacterial isolate was identified as Bacillus cereus strain ROC. Degradation experiments under agitation with both free and immobilized cells indicates that this strain degrades both azo- dyes in 5 days. The immobilized cells were more proficient than their free cell counterparts. The toxicity of the biotransformation products formed after decolorization were assessed by conducting bacteriotoxic and phytotoxic assays. All the toxicity assays indicate that the dyes’ degraded products were non-toxic in nature, as compared to the dyes themselves. The kinetics of the azo dyes’ degradation was also studied at various initial concentration ranges from 50 mg/L to 250 mg/L by growth independent kinetic models. Zero-order kinetics were fit to the experimental data, producing values of least squares regression (R2) greater than 0.98, which indicates that the bacterial strain degrades both dyes by co-metabolism rather than utilizing them as sole energy source. These results indicate that the Bacillus cereus ROC strain has great potential to degrade dye-contaminated water and soil.

show abstract

“…Although immobilization can be detrimental to the activity of cells or enzymes, the immobilization of Lysinibacillus sp. KPB6 in calcium alginate achieved about 98% degradation of Reactive Blue-250 after 48 h as opposed to free cells, which achieved about 95% degradation within 72 h duration [213]. Meanwhile, the enzyme AzoRo, an azoreductase from Rhodococcus opacus 1CP, was immobilized to mesoporous silica which showed significant improvement on its stability, exhibiting activity even on incubation at pH 4 for 60 h and showing better storability [197].…”

Section: Immobilizationmentioning

confidence: 99%

Microbial Degradation of Azo Dyes: Approaches and Prospects for a Hazard-Free Conversion by Microorganisms

Ngo

Tischler

2022

IJERPH

View full text Add to dashboard Cite

Azo dyes have become a staple in various industries, as colors play an important role in consumer choices. However, these dyes pose various health and environmental risks. Although different wastewater treatments are available, the search for more eco-friendly options persists. Bioremediation utilizing microorganisms has been of great interest to researchers and industries, as the transition toward greener solutions has become more in demand through the years. This review tackles the health and environmental repercussions of azo dyes and its metabolites, available biological approaches to eliminate such dyes from the environment with a focus on the use of different microorganisms, enzymes that are involved in the degradation of azo dyes, and recent trends that could be applied for the treatment of azo dyes.

show abstract

A study on the utility of immobilized cells of indigenous bacteria for biodegradation of reactive azo dyes

Cited by 23 publications

References 53 publications

Improved application of immobilized Enterobacter cloacae into a bio‐based polymer for Reactive Blue 19 removal, an eco‐friendly advancement in potential decolorizing systems

Improved application of immobilized Enterobacter cloacae into a bio‐based polymer for Reactive Blue 19 removal, an eco‐friendly advancement in potential decolorizing systems

Decolorization of azo dyes by a novel aerobic bacterial strain Bacillus cereus strain ROC

Microbial Degradation of Azo Dyes: Approaches and Prospects for a Hazard-Free Conversion by Microorganisms

Contact Info

Product

Resources

About