A study on the prevalence of Aeromonas spp. and its enterotoxin genes in samples of well water, tap water, and bottled water

Abstract

Aim:The aim of this work was to study the prevalence of Aeromonas spp. and its enterotoxin genes in various water sources.Materials and Methods:125 samples (50 from well water, 50 from tap water, and 25 from bottled water) were collected from various sources in and around Greater Hyderabad Municipal Corporation and examined for the presence of aeromonads by both cultural and polymerase chain reaction (PCR) assay. Alkaline peptone water with ampicillin was used as enrichment. Aeromonas isolation medium and ampicillin dextrin agar were used as selective media. The boiling and snap chilling method was used for DNA extraction. Primers targeted against 16S rRNA, aer, and ast were used to identify aeromonads and its enterotoxins.Results:48%, 18%, and 12% of well water, tap water, and bottled water samples were found positive by cultural assay with an overall prevalence of 28.8%. Aeromonads were detected in 32 % (52% in well water, 20% in tap water, and 16% in bottled water) of samples by PCR assay. Aerolysin (aer) gene was noticed in 34.6%, 20%, and 0% of well water, tap water, and bottled water samples, respectively, with an overall prevalence of 27.5%. Thermostable cytotonic enterotoxin (ast) was observed in 37.5% (42.3% in well water, 30% in tap water, and 25% in bottled mineral water) of samples.Conclusions:Presence of aeromonads and its toxin genes in various sources of water is of public health concern and emphasizes the need for necessary preventive measures to tackle the problem.

Association Between Antimicrobial Resistance and Biofilm Production of Aeromonas hydrophila Isolated from Beef and Mutton in Duhok Abattoir

“…Higher incidence was reported by Ibrahim et al [41] who found (60%) of beef and (58%) of mutton samples in Australia to be contaminated with this bacterium. The degree of variations in the results obtained by many authors can be due to the disparity of geographical locations, the methods and techniques adopted for isolation, samples collecting seasons [42], nutrition factors, livestock density, breeding systems, methods used in the slaughtering process, and the level of sanitation, which considered among the most important factors that affect the different levels of contamination with A. hydrophila in animal food samples [43]. The findings obtained in this study are concerning because different spp.…”

Genomic Characterization of Aeromonas veronii Provides Insights into Taxonomic Assignment and Reveals Widespread Virulence and Resistance Genes throughout the World

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Multilocus sequence typing and antibiotic resistance of Aeromonas isolated from freshwater fish in Hebei Province

“…Aeromonas spp. are known as opportunistic pathogens capable of causing various diseases in aquatic animals, livestock, and humans and are widely distributed in ecosystems encompassing rivers, lakes, oceans, and urban domestic water [1,2]. Aeromonas spp.…”