A study on the mechanism of agonists in regulating transcriptional level of pIgR in salivary gland epithelial cells

Huang, Li; Sun, Chuankong; Peng, Ruobing; Liu, Zhiming

doi:10.3892/etm.2018.6792

Cited by 3 publications

(3 citation statements)

References 19 publications

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“…SGECs were isolated from salivary glands of mice according to a previous method [20]. Our study was in accordance with institutional guidelines from the Affiliated Hospital of Qingdao University.…”

Section: Isolation and Treatment Of Sgecsmentioning

confidence: 99%

RETRACTED ARTICLE: Mirt2 functions in synergy with miR-377 to participate in inflammatory pathophysiology of Sjögren's syndrome

Xin

Liang

Wang

et al. 2019

Artificial Cells, Nanomedicine, and Biotechnology

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Background: The interaction of long non-coding RNAs (lncRNAs)-microRNAs (miRs) exerts crucial functions in mediating inflammatory reaction. It is still unclear whether myocardial infarction associated transcript 2 (Mirt2)-miR-377 mediates the inflammatory pathogenesis in Sj€ ogren's syndrome (SS). Methods: The inflammatory lesion model was established by stimulating salivary gland epithelial cells (SGECs) by interferon gamma (IFN-c). Mirt2-and/or miR-377-transfected SGECs, as well as their negative controls, were applied to investigate the biological functions in inflammation. Cell viability and apoptosis were examined using commercial kits. Western blot was applied to quantify protein level, and enzyme-linked immuno sorbent assay (ELISA) was used to value the secretion of cytokines. Results: The up-regulation of Mirt2 was observed in IFN-c-treated SGECs. Mirt2 overexpression restored the expression of miR-377 which was repressed by IFN-c. However, miR-377 silence abolished the protective effect on cell viability, inhibitory effect on apoptosis and prohibitive role in pro-inflammatory factors. Mirt2 diminished the phosphorylated expression of crucial regulators while miR-377 silence restored the phosphorylation in IFN-c-treated SGECs. Conclusion: Mirt2 was elevated in IFN-c-treated SGECs and then up-regulated miR-377 in response to inflammatory lesions. Mechanically, in synergy with miR-377 Mirt2 blocked IFN-c-evoked activation of NF-jB and JAK/STAT signalling pathway. ARTICLE HISTORY

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Section: Isolation and Treatment Of Sgecsmentioning

confidence: 99%

RETRACTED ARTICLE: Mirt2 functions in synergy with miR-377 to participate in inflammatory pathophysiology of Sjögren's syndrome

Xin

Liang

Wang

et al. 2019

Artificial Cells, Nanomedicine, and Biotechnology

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“…We confirmed the direct regulation of PIGR in HSG cells by Streptococcus mutans through in vitro experiments. A previous study reported similar results from in vivo experiments: salivary gland epithelial cells isolated from patients infected with oral Streptococci showed reduced Pigr expression (47). Streptococcus mutans is the major cause of dental caries (48); therefore, its inhibitory effect on Pigr is reasonable because it reduces the transportation of anti-caries IgA into the saliva.…”

Section: Discussionsupporting

confidence: 57%

The Alteration of Salivary Immunoglobulin A in Autism Spectrum Disorders

Gong

Qiao

et al. 2021

Front. Psychiatry

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Objectives: Autism spectrum disorders (ASD) are neurodevelopmental disorders with changes in the gut and oral microbiota. Based on the intimate relationship between the oral microbiota and oral mucosal immunity, this study aimed to investigate changes in salivary immunoglobulin A (IgA) level in ASD and the underlying mechanism for any such changes.Methods: We recruited 36 children diagnosed with ASD and 35 normally developing children and measured their salivary IgA content using enzyme-linked immunosorbent assay (ELISA). The valproate (VPA) -treated ASD mouse model was established by prenatal exposure to valproate and mouse salivary IgA content was also quantified by ELISA. The submandibular glands of VPA and control mice were isolated and analyzed using qRT-PCR, immunofluorescence staining, and flow cytometry. ASD-related Streptococci were co-incubated with the human salivary gland (HSG) cell line, and western blotting was used to detect the levels of relevant proteins.Results: We found that salivary IgA content was significantly decreased in patients with ASD and had a significant ASD diagnostic value. The salivary IgA content also decreased in VPA mice and was significantly correlated with autistic-like behaviors among them. The mRNA and protein levels of the polymeric immunoglobulin receptor (Pigr) were downregulated in the submandibular glands of VPA mice and the Pigr mRNA level was positively correlated with mouse salivary IgA content. HSG cells treated with ASD-related Streptococci had reduced PIGR protein level.Conclusion: Therefore, protective IgA levels were reduced in the saliva of individuals with ASD, which correlated with the bacteria-induced downregulation of Pigr in salivary glands. This study suggests a new direction for ASD diagnosis and prevention of oral diseases in ASD cohorts and provides evidence for the ASD mucosal immunophenotype in the oral cavity.

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“…Ovostatin homolog (OVOS) was subjected to IPR enrichment and was found to belong to the immunoglobulin E set. Interestingly, a non-secreted protein, the polymeric immunoglobulin receptor (PIGR), has been reported to be positively correlated with IgA concentrations in mouse saliva ( Huang et al., 2018 ; Gong et al., 2021 ).…”

Section: Resultsmentioning

confidence: 99%

The submandibular and sublingual glands maintain oral microbial homeostasis through multiple antimicrobial proteins

Liu

Guan

et al. 2023

Front. Cell. Infect. Microbiol.

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IntroductionOral microbial homeostasis is a key factor affecting oral health, and saliva plays a significant role in maintaining oral microbial homeostasis. The submandibular gland (SMG) and sublingual gland (SLG) together produce the most saliva at rest. Organic ingredients, including antimicrobial proteins, are rich and distinctive and depend on the type of acinar cells in the SMG and SLG. However, the functions of the SMG and SLG in maintaining oral microbial homeostasis have been difficult to identify and distinguish, given their unique anatomical structuresMethodsIn this study, we independently removed either the SMG or SLG from mouse models. SMGs were aseptically removed in three mice in the SMG-removal group, and SLGs were aseptically removed in three mice in the SLG-removal group. Three mice from the sham-operated group were only anesthetized and incised the skin. After one month, we analyzed their oral microbiome through 16S rRNA sequencing. And then, we analyzed each gland using proteomics and single-cell RNA sequencing.ResultsOur study revealed that the microbiome balance was significantly disturbed, with decreased bacterial richness, diversity, and uniformity in the groups with the SMG or SLG removed compared with the sham-operated group. We identified eight secreted proteins in the SMG and two in the SLG that could be involved in maintaining oral microbial homeostasis. Finally, we identified multiple types of cells in the SMG and SLG (including serous acinar, mucinous acinar, ductal epithelial, mesenchymal, and immune cells) that express potential microbiota homeostasis regulatory proteins. Our results suggest that both the SMG and SLG play crucial roles in maintaining oral microbial homeostasis via excretion. Furthermore, the contribution of the SMG in maintaining oral microbial homeostasis appears to be superior to that of the SLG. These findings also revealed the possible antimicrobial function of gland secreta.DiscussionOur results suggest that control of oral microbial dysbiosis is necessary when the secretory function of the SMG or SLG is impaired. Our study could be the basis for further research on the prevention of oral diseases caused by microbial dysbiosis.

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A study on the mechanism of agonists in regulating transcriptional level of pIgR in salivary gland epithelial cells

Cited by 3 publications

References 19 publications

RETRACTED ARTICLE: Mirt2 functions in synergy with miR-377 to participate in inflammatory pathophysiology of Sjögren's syndrome

RETRACTED ARTICLE: Mirt2 functions in synergy with miR-377 to participate in inflammatory pathophysiology of Sjögren's syndrome

The Alteration of Salivary Immunoglobulin A in Autism Spectrum Disorders

The submandibular and sublingual glands maintain oral microbial homeostasis through multiple antimicrobial proteins

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